Vitamin D Compounds in Human and Bovine Milk

Part of the Advances in Nutritional Research book series (ANUR, volume 4)


During the past decade considerable progress has been made toward an understanding of the transport, measurement, distribution, and molecular action of the antirachitic sterols. As a result of these major advances in our knowledge of vitamin D, many excellent reviews have been written dealing with each of these specific areas of study (Lawson and Emtage, 1974; Favus, 1978; DeLuca and Schnoes, 1976; J. G. Haddad, Jr., this volume).


Human Milk Bovine Milk Milk Whey Cytosolic Receptor Parent Vitamin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations used


vitamin D binding protein


25-hydroxyvitamin D


24,25-dihydroxyvitamin D


25-hydroxyvitamin D3-26,23-lactone


25,26-dihydroxyvitamin D


1,25-dihydroxyvitamin D


gas chromatography


high-performance liquid chromatography


calcium binding protein


recommended daily allowance


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  1. Adachi, A., and Kobayashi, T., 1979, Identification of vitamin D3 and 7-dehydrocholesterol in cow’s milk by gas chromatography-mass spectrometry and their quantitation by high-performance liquid chromatography, J. Nutr. Sci. Vitaminol. 25:67.PubMedCrossRefGoogle Scholar
  2. Armbrecht, H. J., and Wasserman, R. H., 1976, Enhancement of Ca++ uptake by lactose in the rat small intestine, J. Nutr. 106:1265.PubMedGoogle Scholar
  3. Belsey, R., DeLuca, H. F., and Potts, J. T., 1971, Competitive binding assay for vitamin D and 25-OH-vitamin D, J. Lab. Clin. Med. 33:554.Google Scholar
  4. Belsey, R., Clark, M. B., Bernat, M., Glowacki, J., Holick, M. F., DeLuca, H. F., and Potts, J. T., 1974, The physiologic significance of plasma transport of vitamin D and metabolites, Am. J. Med. 57:50.PubMedCrossRefGoogle Scholar
  5. Bouillon, R., Van Baelen, H., and De Moor, P., 1977, The measurement of the vitamin D-binding protein in human serum, J. Clin. Endocrinol. Metab. 45:225.PubMedCrossRefGoogle Scholar
  6. Bouillon, R., Van Baelen, H., Rombauts, W., and De Moor, P., 1978, The isolation and characterization of the vitamin-D-binding protein from rat serum, J. Biol. Chem. 253:4426.PubMedGoogle Scholar
  7. Brumbaugh, P. F., and Haussier, M. R., 1975, Nuclear and cytoplasmic binding components for vitamin D metabolites, Life Sci. 16:353.PubMedCrossRefGoogle Scholar
  8. Burger, R. L., and Allen, R. H., 1974, Characterization of vitamin B12-binding proteins isolated from human milk and saliva by affinity chromatography, J. Biol. Chem. 249:7220.PubMedGoogle Scholar
  9. Christakos, S., and Norman, A. W., 1979, Studies on the mode of action of calciferol XVIII. Evidence for a specific high affinity binding protein for 1,25-dihydroxyvitamin D3 in chick kidney and pancreas, Biochem. Biophys. Res. Commun. 89:56.PubMedCrossRefGoogle Scholar
  10. Cooke, N. E., Walgate, J., and Haddad, J. G., 1979, Human serum binding protein for vitamin D and its metabolites. I. Physiochemical and immunological identification in human tissues, J. Biol. Chem. 254:5958.PubMedGoogle Scholar
  11. DeLuca, H. F., and Schnoes, H. K., 1976, Metabolism and mechanism of action of vitamin D, Annu. Rev. Biochem. 45:631.PubMedCrossRefGoogle Scholar
  12. Eisman, J. A., Hamstra, A. J., Kream, B. E., and DeLuca, H. F., 1976, A sensitive, precise, and convenient method for determination of 1,25-dihydroxyvitamin D in human plasma, Arch. Biochem. Biophys. 176:235.PubMedCrossRefGoogle Scholar
  13. Favus, M. J., 1978, Vitamin D physiology and some clinical aspects of the vitamin D endocrine system, Med. Clin. North Am. 62:1291.PubMedGoogle Scholar
  14. Gast, D. R., Marquardt, J. P., Jorgensen, N. A., and DeLuca, H. F., 1977, Efficacy and safety of 1α-hydroxyvitamin D3 for prevention of parturient paresis, J. Dairy Sci. 60:1910.PubMedCrossRefGoogle Scholar
  15. Haddad, J. G., and Birge, S. J., 1975, Widespread, specific binding of 25-hydroxycholecalciferol in rat tissues, J. Biol. Chem. 250:299.PubMedGoogle Scholar
  16. Haddad, J. G., and Chyu, K. J., 1971, Competitive protein-binding radioassay for 25-hydroxycholecalciferol, J. Clin. Endocrinol. Metab. 33:992.PubMedCrossRefGoogle Scholar
  17. Haddad, J. G., and Walgate, J., 1976, Radioimmunoassay of the binding protein for vitamin D and its metabolites in human serum. Concentrations in normal subjects and patients with disorders of mineral homeostasis, J. Clin. Invest. 58:1217.PubMedCrossRefGoogle Scholar
  18. Haddad, J. G., Hillman, L., and Rojanasathit, S., 1976, Human serum binding capacity and affinity for 25-hydroxyergocalciferol and 25-hydroxycholecalciferol, J. Clin. Endocrinol. Metab. 43:86.PubMedCrossRefGoogle Scholar
  19. Halloran, B. P., and DeLuca, H. F., 1979, Vitamin D deficiency and reproduction in rats, Science 204:73.PubMedCrossRefGoogle Scholar
  20. Halloran, B. P., Barthell, E. N., and DeLuca, H. F., 1979, Vitamin D metabolism during pregnancy and lactation in the rat, Proc. Natl. Acad. Sci. U.S.A. 76:5549.PubMedCrossRefGoogle Scholar
  21. Harris, R. S., and Bunker, J. W. M., 1939, Vitamin D potency of human breast milk, Am. J. Public Health 29:744.CrossRefGoogle Scholar
  22. Hibbs, J. W., and Pounden, W. D., 1955, Studies on milk fever in dairy cows. IV. Prevention by short-time, prepartum feeding of massive doses of vitamin D, J. Dairy Sci. 38:65.CrossRefGoogle Scholar
  23. Higaki, M., Takahashi, M., Suzuki, T., and Sahashi, Y., 1965, Metabolic activities of vitamin D in animals. III. Biogenesis of vitamin D sulfate in animal tissues, J. Nutr. Sci. Vitaminol. 11:261.Google Scholar
  24. Hoff, N., Haddad, J., Teitelbaum, S., McAlister, W., and Hillman, L. S., 1979, Serum concentrations of 25-hydroxyvitamin D in rickets of extremely premature infants, J. Pediatr. 94:460.PubMedCrossRefGoogle Scholar
  25. Hollis, B. W., and Draper, H. H., 1978, Vitamin D metabolites in milk, Fed. Proc. 37:409.Google Scholar
  26. Hollis, B. W., and Draper, H. H., 1979, A comparative study of vitamin D binding globulins in milk, Comp. Biochem. Physiol. 64B:41.Google Scholar
  27. Hollis, B. W., Burton, J. H., and Draper, H. H., 1977a, A binding assay for 25-hydroxycalciferols and 24R,25-dihydroxycalciferols using bovine plasma globulin, Steroids 30:285.CrossRefGoogle Scholar
  28. Hollis, B. W., Hibbs, J. W., and Conrad, M. R., 1977b, Vitamin D binding factors in bovine blood, J. Dairy Sci. 60:1605.CrossRefGoogle Scholar
  29. Hollis, B.W., Lambert, P. W., and Draper, H. H., 1979, On the occurrence of vitamin D sulfate in milk, J. Nutr. 109:XXIV.Google Scholar
  30. Hollis, B. W., Roos, B. A., and Lambert, P. W., 1980a, 25,26-Dihydroxycholecalciferol: A precursor in the renal synthesis of 25-hydroxycholecalciferol-26,23-lactone, Biochem. Biophys. Res. Commun. 95:520.CrossRefGoogle Scholar
  31. Hollis, B. W., Roos, B. A., and Lambert, P. W., 1980b, Isolation and quantitation of vitamin D metabolites in human and bovine milk, Clin. Res. 28:395a.Google Scholar
  32. Hollis, B. W., Roos, B. A., Draper, H. H., and Lambert, P. W., 1981a, On the occurrence of vitamin D sulfate in human milk whey, J. Nutri. 111:384.Google Scholar
  33. Hollis, B. W., Roos, B. A., Draper, H. H., and Lambert, P. W., 1981b, Vitamin D metabolites in human and bovine milk, J. Nutr. 111:1240.Google Scholar
  34. Horst, R. L., 1979, 25-OHD3–26,23-Lactone: A metabolite of vitamin D3 that is 5 times more potent than 25-OHD3 in the rat plasma competitive protein binding radioassay, Biochem. Biophys. Res. Commun. 89:286.PubMedCrossRefGoogle Scholar
  35. Horst, R. L., Shepard, R. M., Jorgensen, N. A., and DeLuca, H. F., 1979, The determination of 24,25-Dihydroxyvitamin D and 25,26-Dihydroxyvitamin D in plasma from normal and nephrec-tomized man, J. Lab. Clin. Med. 93:277.PubMedGoogle Scholar
  36. Hughes, M. R., Baylink, D. J., Jones, P. G., and Haussier, M. R., 1976, Radioligand receptor assay for 25-hydroxyvitamin D2/D3 and 1,25-Dihydroxyvitamin D2/D3. Application to hyper-vitaminosis D, J. Clin. Invest. 58:61.PubMedCrossRefGoogle Scholar
  37. Imawari, M., Kida, K., and Goodman, D. S., 1976, The transport of vitamin D and its 25-hydroxy metabolite in human plasma. Isolation and partial characterization of vitamin D and 25-hydroxyvitamin D binding protein, J. Clin. Invest. 58:514.PubMedCrossRefGoogle Scholar
  38. Jelliffe, D. B., and Jelliffe, E. F., 1978, Human Milk In the Modern World, Oxford University Press, New York.Google Scholar
  39. Kano, K., Yoshida, H., Yata, J., and Suda, T., 1980, Age and seasonal variations in the serum levels of 25-hydroxyvitamin D and 24,25-Dihydroxyvitamin D in normal humans, Endocrinol. J. 27:215.CrossRefGoogle Scholar
  40. Kodicek, E., and Lawson, D. E. M., 1967, Vitamin D, in: The Vitamins (P. Gyorgy and W. N. Pearson, eds.), pp. 211–244, Academic Press, New York.Google Scholar
  41. Kream, B. E., Jose, M., Yamada, S., and DeLuca, H. F., 1977, A specific high-affinity binding macromolecule for 1,25-dihydroxyvitamin D3 in fetal bone, Science 197:1086.PubMedCrossRefGoogle Scholar
  42. Kream, B. E., DeLuca, H. F., Moriality, D. M., Kendrick, N. C., and Ghazarian, J. G., 1979, Origin of 25-hydroxyvitamin D3 binding protein from tissue cytosol preparations, Arch. Biochem. Biophys. 192:318.PubMedCrossRefGoogle Scholar
  43. Kumar, R., Cohen, W. R., Silva, P., and Epstein, F. H., 1979, Elevated 1,25-dihydroxyvitamin D plasma levels in normal human pregnancy and lactation, J. Clin. Invest. 63:342.PubMedCrossRefGoogle Scholar
  44. Lakdawala, D. R., and Widdowson, E. M., 1977, Vitamin D in human milk, Lancet 1:67.Google Scholar
  45. Lambert, P. W., Syverson, B. J., Arnaud, C. D., and Spelsberg, T. C., 1977, Isolation and quantitation of endogenous vitamin D and its physiologically important metabolites in human plasma by high pressure liquid chromatography, J. Steroid Biochem. 8:929.PubMedCrossRefGoogle Scholar
  46. Lambert, P. W., Toft, D. O., Hodgson, S. F., Lindmark, E. A., Witrak, B. J., and Roos, B. A., 1978, An improved method for the measurement of 1,25-(OH)2-D3 in human plasma, Endocr. Res. Commun. 5:293.PubMedCrossRefGoogle Scholar
  47. Lambert, P. W., Hollis, B. W., Bell, N. H., and Epstein, S., 1980, Demonstration of a lack of change in serum 1,25-dihydroxyvitamin D in response to parathyroid extract in pseudohypoparathyroidism, J. Clin. Invest. 66:782.PubMedCrossRefGoogle Scholar
  48. Larson, B. L., and Jorgensen, G. N., 1974, Biosynthesis of the milk proteins, in: Lactation: Biosynthesis and Secretion of Milk (B. L. Larson and V. R. Smith, eds.), pp. 115–146, Academic Press, New York.Google Scholar
  49. Lawson, D. E. M., and Emtage, J. S., 1974, Molecular action of vitamin D in the chick intestine, Vitam. Horm. 32:277.PubMedCrossRefGoogle Scholar
  50. Lawson, D. E. M., Charman, M., Wilson, P. W., and Edelstein, S., 1976, Some characteristics of new tissue-binding proteins for metabolites of vitamin D other than 1,25-dihydroxyvitamin D, Biochem. Biophys. Acta 437:403.PubMedCrossRefGoogle Scholar
  51. LeBoulch, N., Gulat-Marnay, C., and Raoul, Y., 1974, Derives de la vitamine D3 des laits de femme et de vache: Ester sulfate de cholecalciferol et hydroxy-25 cholecalciferol, Int. J. Vitam. Nutr. Res. 44:167.Google Scholar
  52. Leerbeck, E., and Sondergaard, H., 1980, The total content of vitamin D in human milk and cow’s milk, Br. J. Nutr. 44:7.PubMedCrossRefGoogle Scholar
  53. Miravet, L., Redel, J., Carre, M., Queille, M. L., and Bordier, P., 1976, The biological activity of synthetic 25,26-dihydroxycholecalciferol and 24,25-dihydroxycholecalciferol in vitamin D-deficient rats, Calcif. Tissue Res. 21:145.PubMedCrossRefGoogle Scholar
  54. Morrissey, R. L., Zolock, D. T., Bikle, D. D., Empson, R. N., and Bucci, T. J., 1978, Intestinal response to 1,25-dihydroxycholecalciferol. I. RNA polymerase, alksline phosphatase, calcium and phosphorus uptake in vitro, and in vivo calcium transport and accumulation, Biochim. Biophys. Acta 538:23.PubMedCrossRefGoogle Scholar
  55. Noff, D., and Edelstein, S., 1978, Vitamin D and its hydroxylated metabolites in the rat. Placental and lacteal transport, subsequent metabolic pathways and tissue distribution, Horm. Res. 9:292.PubMedCrossRefGoogle Scholar
  56. Norman, A. W., and Henry, H. H., 1974, 1,25-Dihydroxycholecalciferol: A hormonally active form of vitamin D3, Recent Prog. Horm. Res. 30:431.PubMedGoogle Scholar
  57. Osborn, T. W., and Norman, A. W., 1977, A “25-hydroxycholecalciferal-like compound” in milk as determined by specific competitive binding radioassays, in: Vitamin D: Biochemical, Chemical and Clinical Aspects Related to Calcium Metabolism (A. W. Norman, ed.), pp. 523–525, deGruyter, Berlin.Google Scholar
  58. Pansu, D., Bellaton, C., and Bronner, F., 1979, Effect of lactose on duodenal calcium-binding protein and calcium absorption, J. Nutr. 109:508.PubMedGoogle Scholar
  59. Payne, D. W., Peng, L. H., and Pearlman, W. H., 1976, Corticosteroid-binding proteins in human colostrum and milk and rat milk, J. Biol. Chem. 251:5272.PubMedGoogle Scholar
  60. Polskin, L. J., Kramer, B., and Sobel, A. E., 1945, Secretion of vitamin D in milks of women fed fish liver oil, J. Nutr. 30:451.PubMedGoogle Scholar
  61. Porter, J. W. G., 1978, Milk as a source of lactose, vitamin and minerals, Proc. Nutr. Soc. 37:225.PubMedCrossRefGoogle Scholar
  62. Reinhardt, T. A., and Conrad, H. R., 1980, Specific binding protein for 1,25-dihydroxyvitamin D3 in bovine mammary gland, Arch. Biochem. Biophys. 203:108.PubMedCrossRefGoogle Scholar
  63. Sahashi, Y., Suzuki, T., Higaki, M., and Asano, T., 1967a, Metabolism of vitamin D in animals. V. Isolation of vitamin D sulfate from mammlian milk, J. Vitaminol. 13:33.CrossRefGoogle Scholar
  64. Sahashi, Y., Suzuki, T., Higaki, M., Takahashi, M., Asano, T., Hasegawa, T., and Miyazawa, E., 1967b, Metabolic activities of Vitamin D in animals. VI. Physiological activities of vitamin D sulfate, J. Vitaminol. 13:37.CrossRefGoogle Scholar
  65. Schaafsma, G., and Visser, R., 1980, Nutritional interrelationships between calcium, phosphorus and lactose in rats, J. Nutr. 110:1101.PubMedGoogle Scholar
  66. Shepard, R. M., Horst, R. L., Hamstra, A. J., and DeLuca, H. F., 1979, Determination of vitamin D and its metabolites in plasma from normal and anephric man, Biochem. J. 182:55.PubMedGoogle Scholar
  67. Toverud, S. U., and Boass, A., 1979, Hormonal control of calcium metabolism in lactation, Vitam. Horm. 37:303.PubMedCrossRefGoogle Scholar
  68. Ueng, T.-H., Golub, E. E., and Bronner, F., 1979, The effect of age and 1,25-dihydroxyvitamin D3 treatment on the intestinal calcium-binding protein of suckling rats, Arch. Biochem. Biophys. 196:624.PubMedCrossRefGoogle Scholar
  69. Van Baelen, H., Bouillon, R., and DeMoor, P., 1977, Binding of 25-hydroxycholecalciferol in tissues, J. Biol. Chem. 252:2515.PubMedGoogle Scholar
  70. Van Baelen, H., Bouillon, R., and DeMoor, P., 1980, Vitamin D-binding protein (GC-globulin) binds actin, J. Biol. Chem. 255:2270.PubMedGoogle Scholar
  71. Waxman, S., 1975, Folate binding proteins, Br. J. Haematol. 29:23.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  1. 1.Department of MedicineCase Western Reserve UniversityClevelandUSA
  2. 2.Department of Medicine, Division of Endocrinology and Mineral MetabolismVeterans Administration Medical CenterClevelandUSA

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