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Fusion of Enveloped Viruses with Biological Membranes Fluorescence Dequenching Studies

  • Nor Chejanovsky
  • Ofer Nussbaum
  • Abraham Loyter
  • Robert Blumenthal
Chapter
Part of the Subcellular Biochemistry book series (SCBI, volume 13)

Abstract

Enveloped virions penetrate eukaryotic cells by two alternative routes (Chop-pin and Scheid, 1980; White et al., 1983). Envelopes of viruses belonging to the paramyxovirus group fuse with cells’ plasma membranes at pH 7.4 and consequently microinject their content, the viral nucleocapsid, directly into the cell cytoplasm (Choppin and Scheid, 1980; Loyter and Volsky, 1982; White et al., 1983). A different way of entry has been described for most other enveloped virions such as those belonging to the orthomyxovirus, toga, rhabdo, and herpes groups. Such viruses are taken into cells by endocyticlike processes. Fusion of the viral envelopes with the endosomal or lysosomal membranes is triggered by the intraorganelle low-pH environment and leads to the introduction of the viral content into the intracellular space (Chopin and Scheid, 1980; White et al., 1983). Fusion of the pH-dependent virions with the plasma membrane can be triggered by lowering the pH of the medium containing the virus-associated cells.

Keywords

Influenza Virion Membrane Fusion Vesicular Stomatitis Virus Viral Glycoprotein Sendai Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

C12E8

octaethyleneglycolmono(n-dodecyl)ether

CF

carboxyfluorescein

chol

cholesterol

CL

cardiolipin

DPA

dipicolinic acid

DQ

dequenching

DTT

dithiothreitol

EDTA

ethylenediaminetetracetic acid

FRET

fluorescence resonance energy transfer

gang

gangliosides

HA

hemagglutinin

HA0

precursor of the influen hemagglutinin glycoprotein

HEG

human erythrocyte ghosts

HSV

herpes simplex virus

HTC

hepatoma tissue cultured

N-NBD

7-nitro-1,2,3-benzoxydiazole-4-amino

PC

phosphatidylcholine

PE

phosphatidylethanolamine

PMSF

Phenylmethylsulfonyl fluoride

PS

phosphatidylserine

R18

octadecylrhodamine B-chloride

RIVE

reconstituted influenza virus envelope

ROVs

right-side-out (erythrocyte membrane) vesicles

RSVE

reconstituted sendai virus envelope

SFV

Semliki Forest virus

VSV

vesicular stomatitis virus

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Copyright information

© Plenum Press, New York 1988

Authors and Affiliations

  • Nor Chejanovsky
    • 1
  • Ofer Nussbaum
    • 1
  • Abraham Loyter
    • 1
  • Robert Blumenthal
    • 2
  1. 1.Institute of Life SciencesThe Hebrew University of JerusalemJerusalemIsrael
  2. 2.National Cancer InstituteNational Institutes of HealthBethesdaUSA

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