Biochemical Dissection of the Cerebellum—Isolation of Perikarya from the Cerebellum with Well-Preserved Ultrastructure

  • Robert Balázs
  • G. P. Wilkin
  • J. E. Wilson
  • J. Cohen
  • G. R. Dutton
Chapter

Abstract

Our understanding of metabolic compartmentation in the brain would be greatly advanced by information about the biochemical properties of specific cell types. Although several techniques for isolating cell bodies from the CNS are available, none yield cells that exhibit a fully acceptable level of ultrastructural preservation (for reviews see Johnston and Roots, 1970; Rose, 1973; Hamberger and Henn, 1973), This chapter describes our efforts to obtain isolated perikarya from rat cerebellum, which maintain a high degree of ultrastructural integrity in comparison with cells in situ.

Keywords

Cell Body Purkinje Cell Velocity Sedimentation Purkinje Cell Body Ultrastructural Preservation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Plenum Press, New York 1975

Authors and Affiliations

  • Robert Balázs
    • 1
  • G. P. Wilkin
    • 1
  • J. E. Wilson
    • 2
  • J. Cohen
    • 3
  • G. R. Dutton
    • 3
  1. 1.MRC Developmental Neurobiology UnitMedical Research Council LaboratoriesCarshalton SurreyEngland
  2. 2.Department of BiochemistryMichigan State UniversityEast LansingUSA
  3. 3.Brain Research Group Department of BiologyThe Open UniversityMilton Keynes BucksEngland

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