Mouse Skin Carcinogenesis: Application to the Analysis of Complex Mixtures

  • S. Nesnow
  • L. L. Triplett
  • T. J. Slaga
Chapter
Part of the Environmental Science Research book series (ESRH, volume 27)

Abstract

Current information suggests that chemical carcinogenesis is a multistage process; in this regard, one of the best studied models is the two-stage carcinogenesis system in mouse skin. Skin tumors can be induced by the sequential application of a subthreshold dose of a carcinogen (initiation phase) followed by repetitive treatments with a noncarcinogenic tumor promoter (promotion phase). The initiation phase requires only a single application of either a direct or indirect carcinogen at a subthreshold dose and is essentially irreversible. The promotion phase is brought about by repetitive treatments after initiation and is initially reversible but later irreversible. The mouse skin system can be used not only to determine the tumor-initiating and tumor-promoting activities of a compound: if the agent is given repeatedly by itself, one can also determine If it is a complete carcinogen (i.e., if it has both tumor-initiating and tumor-promoting activities).

Keywords

Mouse Skin Coke Oven Skin Carcinogenesis Tumor Multiplicity Carcinogenic Potency 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References

  1. Boutwell, R.K. 1964. Some biological aspects of skin carcinogenesis. Progr. Exp. Tumor Res. 4:207–250.Google Scholar
  2. Campbell, J.A. 1939. Carcinogenic agents present in the atmosphere and incidence of primary lung tumours in mice. Brit. J. Exp. Path. 20:122–132.Google Scholar
  3. Campbell, J.A. 1934. Cancer of skin and increase in incidence of primary tumours of lung in mice exposed to dust obtained from tarred roads. Brit. J. Exp. Path. 15:287–294.Google Scholar
  4. DiGiovanni, J., T.J. Slaga, and R.K. Boutwell. 1980. Comparison of the tumor-initiating activity of 7,12-dimethylbenz(a)-anthracene and benzo(a)pyrene in female SENCAR and CD-I mice. Carcinogenesis 1:381–389.CrossRefGoogle Scholar
  5. Drinkwater, N.R., and J.H. Klotz. 1981. Statistical methods for the analysis of tumor multiplicity data. Cancer Res. 41:113–119.Google Scholar
  6. Finney, P.J., 1971. Probit Analysis. Cambridge University Press: Cambridge, MA.MATHGoogle Scholar
  7. Gart, J.J., K.C. Chu, and R.E. Tarone. 1979. Statistical issues in interpretation of chronic bioassay tests for carcinogenicity. J. Natl. Cancer Inst. 62:957–974.Google Scholar
  8. Hasselblad, V., A.G. Stead, and J.P. Creason. 1980. Multiple probit analysis with a nonzero background. Biometrics 36:659–663.CrossRefGoogle Scholar
  9. Hennings, H., D. Devor, M.L. Wenk, T.J. Slaga, B. Former, N.H. Colburn, G.T. Bowen, E. Kjell, and S.H. Yuspa. 1981. Comparison of two stage epidermal carcinogenesis initiated by 7,12-dimethylbenz(a)anthracene or N-methyl-N’-nitro-N-nitro-soguanidine in newborn and adult SENCAR and BALB/c mice. Cancer Res. 41:773–779.Google Scholar
  10. Hoffmann, D., and E.L. Wynder. 1963. Studies on gasoline engine exhaust. J. Air Pollut. Contr. Assoc. 13:322–327.Google Scholar
  11. Hoffmann, D., E. Theisz, and E.L. Wynder. 1965. Studies on the carcinogenicity of gasoline exhaust. J. Air Pollut. Contr. Assoc. 15:162–165.Google Scholar
  12. Holland, J.M., D.A. Wolf, and B.A. Clark. 1981. Relative potency estimation for synthetic petroleum skin carcinogens. Environ. Health Perspect. 38:149–155.CrossRefGoogle Scholar
  13. Huisingh, J.L., R.L.Bradow, R.H. Jungers, B.D. Harris, R.B. Zweidinger, K.M. Cushing, B.E. Gill, and R.E. Albert. 1980. Mutagenic and carcinogenic potency of extracts of diesel and related environmental emissions: Study design, sample generation, collection, and preparation, (abstr.). In: Health Effects of Diesel Engine Emissions. Proceedings of an International Symposium, Volume 2. W.E. Pepelko, R.M. Danner, and N.A. Clarke, eds. EPA-600/9–80–057b. U.S. Environmental Protection Agency: Washington, DC. pp. 788–800.Google Scholar
  14. IARC Monographs on the Evaluation of the Carcinogenic Risk of Chemicals to Man, Volume 9. 1975. International Agency for Research on Cancer: Lyons, France, pp. 95–105.Google Scholar
  15. IARC Monographs on the Evaluation of the Carcinogenic Risk of Chemicals to Man, Volume 7. 1974. International Agency for Research on Cancer: Lyons, France, pp. 111–140.Google Scholar
  16. Kotiti, P., H.L. Falk, and M. Thomas. 1955. Aromatic hydrocarbons, III: presence in the particulate phase of diesel-engine exhausts and the carcinogenicity of exhaust extracts. AMA Arch. Ind. Health 11:113–120.Google Scholar
  17. Kotin, P., H.L. Falk, and M. Thomas. 1954a. Aromatic hydrocarbons, II: presence in the particulate phase of gasoline-engine exhausts and the carcinogenicity of exhaust extracts. AMA Arch. Ind. Hyg. Occup. Med. 9:164–177.Google Scholar
  18. Kotin, P., H.L. Falk, M.P. Mader, and M. Thomas. 1954b. Aromatic hydrocarbons, I: presence in the Los Angeles atmosphere and the carcinogenicity of atmospheric extracts. AMA Arch. Ind. Hyg. Occup. Med. 9:153–163.Google Scholar
  19. Menster, M., and A.G. Sharkey, Jr. 1977. Chemical Characterization of Diesel Exhaust Particulates. PERC/RI-77/5. ERDA Technical Information Center: Oak Ridge, TN.Google Scholar
  20. Nesnow, S., L. Triplett, and T.J. Slaga. (in press). Comparative tumor-initiating activity of complex mixtures from environmental particulate emissions on SENCAR mouse skin. J. Natl. Cancer Inst.Google Scholar
  21. Nesnow, S., C. Evans, A. Stead, J. Creason, T.J. Slaga, and L.L. Triplett. 1982. Skin carcinogenesis studies of eemission extracts. In: Toxicological Effects of Emissions from Diesel Engines. J. Lewtas, ed. Elsevier-North Holland: New York. pp. 295–320.Google Scholar
  22. Nesnow, S., L.L. Triplett, and T.J. Slaga. 1981. Tumorigenesis of diesel exhaust, gasoline exhaust, and related emission extracts on SENCAR mouse skin. In: Short-Term Bioassays in the Analysis of Complex Environmental Mixtures II. M.D. Waters, S.S. Sandhu, J.L. Huisingh, L. Claxton, and S. Nesnow, eds. Plenum Press: New York. pp. 277–297.Google Scholar
  23. Passey, R.D. 1922. Experimental soot cancer. Brit. Med. J. 2:1112–1113.Google Scholar
  24. Pereira, M.A. 1982. Skin tumorigenesis research data base. J. Amer. Coll. Toxicol. 1:47–82.Google Scholar
  25. Reiners, J., K. Davidson, K. Nelson, M. Mamrack, and T.J. Slaga. (in press). Skin tumor promotion: a comparative study of several stocks and strains of mice. In: Organ and Species Specificity in Chemical Carcinogenesis. R. Langenbach, S. Nesnow, and J.M. Rice, eds. Plenum Press: New York.Google Scholar
  26. Slaga, T.J., and S.M. Fischer, (in press). Strain differences and solvent effects in mouse skin carcinogenesis experiments using carcinogens, tumor initiators and promoters. Progr. Exp. Tumor Res.Google Scholar
  27. Slaga, T.J., A. Sivak, and R.K. Boutwell, eds. 1978. Carcinogenesis: A Comprehensive Survey, Volume 2: Mechanisms of Tumor Promotion and Cocarcinogenesis. Raven Press: New York.Google Scholar
  28. Slaga, T.J., L.L. Triplett, and S. Nesnow. 1981. Mutagenic and carcinogenic potency of extracts of diesel and related environmental emissions: two stage carcinogenesis in skin tumor sensitive mice (SENCAR). Environ. Int. 5:417–424.CrossRefGoogle Scholar
  29. Slaga, T.J., S.M. Fischer, L.L. Triplett, and S. Nesnow. 1982. Comparison of complete carcinogenesis and tumor initiation and promotion in mouse skin: the induction of papillomas by tumor initiation-promotion, a reliable short term assay. J. Amer. Coll. Toxicol. 1:83–100.Google Scholar
  30. Slaga, T.J., L.L. Triplett, and S. Nesnow. 1980a. Mutagenic and carcinogenic potency of extracts of diesel and related environmental emissions: two-stage carcinogenesis in skin tumor sensitive mice (SENCAR). (abstr.). In: Health Effects of Diesel Engine Emissions. Proceedings of an International Symposium, Volume 2. W.E. Pepelko, R.M. Danner, and N.A. Clarke, eds. EPA-600/9–80–057b. U.S. Environmental Protection Agency: Washington, DC. pp. 874–897.Google Scholar
  31. Slaga, T.J., G.L. Gleason, G. Mills, L. Ewald, P.P. Fu, H.M. Lee, and R.G. Harvey. 1980b. Comparison of the skin tumor-initiating activities of dihydrodiols and diol-epoxides of various polycyclic aromatic hydrocarbons. Cancer Res. 40:1981–1984.Google Scholar
  32. Stead, A.G., V. Hasselblad, J.P. Creason, and L. Claxton. 1981. Modeling the Ames test. Mutation Res. 85:13–27.Google Scholar
  33. Stenback, F. 1980. Skin carcinogenesis as a model system; observations on species, strain and tissue sensitivity to 7,12-dimethylbenz(a)anthracene with and without promotion from croton oil. Acta Pharmacol. Toxicol. 46:89–97.CrossRefGoogle Scholar
  34. Totter, J.R., and F.J. Finamore. 1978. Dose response to cancerogenic and mutagenic treatment. Environ. Int. 1:233–244.CrossRefGoogle Scholar
  35. Von Haam, E., and F.S. Mallette. 1952. Studies on the toxicity and skin effects of compounds used In the rubber and plastics industries, III: carcinogenicity of carbon black extracts. Ind. Hyg. Occup. Med. 6:237–242.Google Scholar
  36. Vosamae, A.I. 1979. Carcinogenicity studies of Estonian oil shale soots. Environ. Health Perspect. 30:173–176.Google Scholar
  37. Wynder, E.L., and D. Hoffmann. 1965. Some laboratory and epidemiological aspects of air pollution carcinogenesis. J. Air Pollut. Control Assoc. 15:155–159.Google Scholar
  38. Wynder, E.L., and D. Hoffman. 1962. A study of air pollution carcinogenesis, III: carcinogenic activity of gasoline engine exhaust condensate. Cancer 15:103–108.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • S. Nesnow
    • 1
  • L. L. Triplett
    • 2
  • T. J. Slaga
    • 2
  1. 1.Carcinogenesis and Metabolism Branch (MD 68)U.S. Environmental Protection AgencyResearch Triangle ParkUSA
  2. 2.Biology DivisionOak Ridge National LaboratoryOak RidgeUSA

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