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Relaxin pp 273-282 | Cite as

Effect of Porcine Relaxin on Cyclic Nucleotide Levels and Spontaneous Contractions of the Rat Uterus

  • B. M. Sanborn
  • H. S. Kuo
  • N. W. Weisbrodt
  • O. D. Sherwood
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 143)

Abstract

Relaxin has been known for some time to inhibit spontaneous uterine contractions in a number of species (Schwabe et al., 1978). Furthermore a number of studies have indicated that circulating factors in addition to steroid hormones regulate uterine contractility and responsiveness during pregnancy (Porter, 1972; Korenman et al., 1974; Porter and Downing, 1978). In the human, serum relaxin levels are elevated early in pregnancy, suggesting that this hormone may play a role in maintaining uterine quiescence during that period (Quagliarello et al., 1979).

Keywords

Cyclic Nucleotide Adenylate Cyclase Activity Spontaneous Contraction Estradiol Benzoate Uterine Contractility 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Andersson, R. G. G. and Nilsson, K. B. (1977). Role of cyclic nucleotides: Metabolism and mechanical activity in smooth muscle, In: The Biochemistry of Smooth Muscle, N. L. Stephans, ed., University Park Press, Baltimore, MD. p. 263.Google Scholar
  2. Braddon, S. A. (1978).. Relaxin-dependent adenosine 3′,5′-mono-phosphate concentration changes in the mouse pubic symphysis, Endocrinology 102: 1292.CrossRefGoogle Scholar
  3. Bryant-Greenwood, G, Mercado-Simmen, Rosalia, Yamamoto, Sandra Y., Arakaki, R. F., Uchima, F. D. A. and Greenwood, F. C. (1981). Relaxin receptors and a study.of the physiological roles of relaxin. 15th Midwest Conference on Endocrin. and Metab.Google Scholar
  4. Cheah, S. H. and Sherwood, O. D. (1980). Target tissues relaxin in the rat: Tissue distribution of injected 125I- labeled relaxin and tissue changes in cyclic AMP levels after in vitro relaxin incubation. Endocrinology 106: 1203CrossRefGoogle Scholar
  5. Diamond, J. (1977). Evidence for dissociation between cyclic nucleotide levels and tension in smooth muscle. In: “The Biochemistry of Smooth Muscle” H. L. Stephans, ed., University Park Press, Baltimore, MD. p. 343.Google Scholar
  6. Diamond, J. and Hartle, K. D. (1976). Cyclic nucleotide levels during carbachol-induced smooth muscle contractions. J. Cycl. Nucleotide Res. 2: 179.Google Scholar
  7. Heindel, J. J., Hiritz, M. I., Steinberger, E. and Strada, S. J. (1978). The effect of FSH on cyclic nucleotide levels in testicular tissue from rats of various ages: Non correlation with cyclic nucleotide phosphodiesterase activity. Endocrine Res. Commun. 4: 6.Google Scholar
  8. Korenman, S. G., Bhalla, R. C., Wortsman, J., Stevens, R., Wells, L.and Carpenter, L. (1974). Hormonal regulation of uterine contractility: The role of the adenylate cyclase system. J. Steroid Biochem. 5: 905.Google Scholar
  9. Korenman, S. G. and Krall, J. F. (1977). The role of cyclic AMP in the regulation of smooth muscle cell contraction in the uterus. Biol. Reprod. 16:1.CrossRefGoogle Scholar
  10. Layne, E. (1957). Protein estimation with the Folin-Ciocalteu method, In: Methods in Enzymology. S. P. Colowick and N. 0. Kaplan, ed. Vol. Ill, Academic Press, NY p. 448.Google Scholar
  11. McMurtry, J. P., Kwok, S. C. M. and Bryant-Greenwood, G. Ij (1978). Target tissues for relaxin identified in vitro with I- labelled porcine relaxin. J. Reprod. Fert. 52: 209.Google Scholar
  12. Paterson, G. (1965). The nature of the inhibition of the rat uterus by relaxin. J. Pharm. Pharmacol. 17:262.CrossRefGoogle Scholar
  13. Porter, D. G. (1972). Myometrium of the pregnant guinea pig: The probable importance of relaxin. Biol. Reprod. 7:458.CrossRefGoogle Scholar
  14. Porter, D. G. and Downing, S. J. (1978). Evidence that a humoral factor possessing relaxin like activity is responsible for uterine quiescence in the late pregnant rat. J. Reprod. Fert. 52: 95.CrossRefGoogle Scholar
  15. Quagliarello, J., Steinetz, F. G. and Weiss, G. (1979). Relaxin secretion in early pregnancy. Obstet. Gyn. 53:62.Google Scholar
  16. Rudzik, A. D and Miller, J. W. (1962a). The mechanism of uterine inhibitory action of relaxin-containing ovarian extracts. J. Pharmacol. Exp. Ther. 138: 82.PubMedGoogle Scholar
  17. Rudzik, A. D. and Miller, J. W. (1962b). The effect of altering the catecholamine content of the uterus on the rate of contractions and the sensitivity of the myometrium to relaxin. J. Pharmacol. Exp. Ther. 138: 88.PubMedGoogle Scholar
  18. Salomon, Y., Londos, C. and Rodbell, M. (1974). A highly sensitive adenylate cyclase assay. Analyt. Biochem. 58:541.CrossRefGoogle Scholar
  19. Salomon, Y., Londos, C. and Rodbell, M. (1974). A highly sensitive adenylate cyclase assay. Analyt. Biochem. 58: 541.CrossRefGoogle Scholar
  20. Sanborn, B. M., Kuo, H. S., Weisbrodt, N. W. and Sherwood, 0. D. (1980). The interaction of relaxin with the rat uterus. I: Effect on cyclic nucleotide levels and spontaneous con-tractile activity. Endocrinology 106: 1210.CrossRefGoogle Scholar
  21. Schwabe, C., Steinetz, B., Weiss, G., Segaloff, A., McDonald, J. K., O’Bryne, E., Hochman, J., Carriere, J. and Goldsmith, L. (1978). Relaxin. Recent Prog. Horm. Res. 34: 123.PubMedGoogle Scholar
  22. Thompson, W. J., Williams, R. H. and Little, S. A. (1973). Studies on the assay and activities of guanyl and adenyl cyclase of rat liver. Arch. Biochem. Biophys. 159:206.CrossRefGoogle Scholar
  23. Harbon, S., Vesin, M. F., Khac, L. D. and Leiber, D. (1978). Cyclic nucleotides in the regulation of rat uterus contractility. In: Molecular Biology and Pharmacology of Cyclic Nucleotides, G. Folco and R. Paolette, eds., Elsevier, Holland. p. 279.Google Scholar

Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • B. M. Sanborn
    • 1
  • H. S. Kuo
    • 1
  • N. W. Weisbrodt
    • 2
  • O. D. Sherwood
    • 3
  1. 1.Departments of Reproductive Medicine and BiologyThe University of Texas Medical School at HoustonHoustonUSA
  2. 2.Department of PhysiologyThe University of Texas Medical School at HoustonHoustonUSA
  3. 3.Department of Physiology and Biophysics, School of Basic Medical SciencesUniversity of IllinoisUrbanaUSA

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