Recombinant DNA Research and Viruses pp 173-197 | Cite as
Cloning of Papillomavirus DNA
Abstract
Papillomaviruses induce epithelial proliferation in man and in a number of animal species (for review see 1, 2). Replication of the virus particles occurs within those tumors, but is restricted to the upper layers of the epidermis (3, 4) indicating that only cells at a certain level of differentiation can support the virus multiplication. This might be the reason for the lack of an in vitro system to propagate the virus particles (for review see 5). Thus, investigation of papillomaviruses depends upon their isolation from biopsy material which contain — as a property of the virus type-different quantities of virus particles. Even those human papillomaviruses which are of special interest because their association with malignant tumors is suggested by epidemiological or histological studies (for review see 6) are poorly replicated in the respective lesions. Therefore the availability of molecularly cloned viral DNA is a prerequisite to investigate the physical organization as well as the biological behaviour of these viruses in more detail.
Keywords
Cold Spring Harbor Laboratory Genital Wart Verrucous Carcinoma Laryngeal Papilloma Common WartPreview
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