Environmental Influences on the Development of Sympathetic Neurons

  • Story C. Landis
Chapter
Part of the Current Topics in Neurobiology book series (CTNB)

Abstract

Peripheral autonomic ganglia provide an excellent source of neurons for tissue culture. The ganglia represent, in most cases, discrete and easily dissected packets of neurons. These packets contain relatively homogeneous populations of neurons whose neurotransmitter properties are largely known and for which a number of assays, cytochemical, biochemical, and electrophysiological, are readily available. In the case of sympathetic ganglia, the neurons are still relatively immature at birth and therefore amenable to dissociation and culturing. Further, the trophic factor, nerve growth factor (NGF), for these cells is not only known but can readily be purified in reasonable quantities from a relatively accessible source. All of these advantages have in aggregate allowed the exploitation of a cell culture system for dissociated neurons from the superior cervical ganglion (SCG) of the newborn rat. This system has yielded a number of new insights into neurotransmitter plasticity and stability, multiple neurotransmitter function, and environmental influences on development. The purpose of this review is to summarize what has been learned from this culture system and to briefly describe recent studies aimed at establishing the relevance of these findings to normal development in the intact animal.

Keywords

Nerve Growth Factor Sweat Gland Sympathetic Neuron Superior Cervical Ganglion Nonneuronal Cell 
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Copyright information

© Plenum Press, New York 1985

Authors and Affiliations

  • Story C. Landis
    • 1
  1. 1.Department of NeurobiologyHarvard Medical SchoolBostonUSA

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