Regulation of Tubulin Expression in Brain

  • Marcelle R. Morrison
  • W. Sue T. Griffin
Chapter

Abstract

The α- and β-tubulins are the major polypeptide components of microtubules. In the immature nervous system, microtubules are involved in cell division, in the determination of cell shape, and in neuronal growth and migration. In the mature nervous system, microtubules are also involved in synaptogenesis and in axonal transport (Olmsted and Borisy, 1973; Dustin, 1978).

Keywords

Granule Cell Translation Product Tubulin Gene Mouse Neuroblastoma Cell Brain Tubulin 
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References

  1. Addison, W. H. F., 1911, The development of the Purkinje Cells and of the cortical layers in the cerebellum of the albino rat, J. Comp. Neurol. 21: 459.Google Scholar
  2. Altman, J., 1972, Postnatal development of the cerebellar cortex in the rat, J. Comp. Neurol. 145: 353.PubMedGoogle Scholar
  3. Amir-Zaltsman, Y., Ezra, E., Scherson, T., Zutra, A., Littauer, U. Z., and Salomon, Y., 1982, ADP-ribosylation of microtubule proteins as catalyzed by cholera toxin, EMBO J. 1: 181.PubMedGoogle Scholar
  4. Amos, L. A., 1979, Structure of microtubules, in: Microtubules ( K. Roberts and J. S. Hyams, eds.), pp. 1–64, Academic Press, New York.Google Scholar
  5. Arce, C. A., and Barra, H. S., 1985, Release of C-terminal tyrosine from tubulin and microtubules at steady state, Biochem.J. 226: 311.PubMedGoogle Scholar
  6. Barra, H. S., Arce, C. A., Rodriguez, J. A., and Caputto, R., 1974, Some common propertied of the protein that incorporates tyrosine as a single unit and the microtubule proteins, Biochem. Biophys. Res. Commun. 60: 1384.PubMedGoogle Scholar
  7. Berry, M., Rogers, A. W., and Eayrs, J. T., 1964, Pattern of Cell migration during cortical histogenesis, Nature 203: 591.PubMedGoogle Scholar
  8. Black, M. M., and Kurdyla, J. T., 1983, Post-translational modification of tubulin in cultured neurons, Brain Res. 289: 355.PubMedGoogle Scholar
  9. Bond, J. F., and Farmer, S. R., 1983, Regulation of tubulin and actin mRNA production in ratbrain: Expression of a new β-tubulin mRNA with development, Mol. Cell Biol. 3: 1333.PubMedGoogle Scholar
  10. Bond, J. F., Robinson, G. S., and Farmer, S. R., 1984, Differential expression of two neural Cell- specific β-tubulin mRNAs during rat brain development, Mol. Cell. Biol. 4: 1313.PubMedGoogle Scholar
  11. Brady, S. T., Tytell, M., and Lasek, R. J., 1984, Axonal tubulin and axonal microtubules: Biochemical evidence for cold stability, J. Cell Biol. 99: 1716.PubMedGoogle Scholar
  12. Brown, B. A., Nixon, R. A., and Marotta, C. A., 1982, Posttranslational processing of a-tubulin during axoplasmic transport in CNS axons, J. Cell Biol. 94: 159.PubMedGoogle Scholar
  13. Bryan, J., 1974, Biochemical properties of microtubules, Fed. Proc. 33: 152.PubMedGoogle Scholar
  14. Bryan, J., and Wilson, L., 1971, Are cytoplasmic microtubules heteropolymers? Proc. Natl. Acad. Sci. USA 68: 1762.PubMedGoogle Scholar
  15. Bryan, R. N., Cutter, G. A., and Hayashi, M., 1978, Separate mRNAs code for tubulin subunits, Nature 272: 81.PubMedGoogle Scholar
  16. Caceres, A., Binder, L. I., Payne, M. R., Bender, P., Rebhun, L., and Steward, O., 1984, Differential subCellular localization of tubulin and the microtubule-associated protein MAP2 in brain tissue as revealed by immunocytochemistry with monoclonal hybridoma antibodies, J. Neurosci. 4: 394.PubMedGoogle Scholar
  17. Campagnoni, C. W., Carey, G. D., and Campagnoni, A. T., 1978, Synthesis of myelin basic proteins in the developing mouse brain, Arch. Biochem. Biophys. 190: 118.PubMedGoogle Scholar
  18. Chaudhury, S., and Sarkar, P. K., 1983, Stimulation of tubulin synthesis by thyroid hormone in the developing rat brain, Biochim. Biophys. Acta 763: 93.PubMedGoogle Scholar
  19. Clark, B. R., Weichsel, M. E., Jr., and Poland, R. E., 1978, Correlation of DNA accumulation rate with thymidylate synthetase activity in developing rat cerebellum: Effect of hypothyroidism, Biol Neonate 34: 209.PubMedGoogle Scholar
  20. Cleveland, D. W., and Sullivan, K. F., 1985, Molecular biology and genetics of tubulin, Ann. Rev. Biochem. 54: 331.PubMedGoogle Scholar
  21. Cleveland, D. W., Lopata, M. A., MacDonald, R. J., Cowan, N. J., Rutter, W. J., and Kirschner, M. W., 1980, Number and evolutionary conservation of a- and β-tubulin and cytoplasmic and γ-actin genes using specific cloned cDNA probes, Cell 20: 95.PubMedGoogle Scholar
  22. Cleveland, D. W., Hughes, S. H., Stubblefield, E., Kirschner, M. W., and Varmus, H. E., 1981, Multiple a and 0 tubulin genes represent unlinked and dispersed gene families, J. Biol. Chem. 256: 3130.PubMedGoogle Scholar
  23. Cowan, N. J., 1983, Tubulin isotypes and the multigene tubulin families, Int. Rev. Cytol. 85: 147.PubMedGoogle Scholar
  24. Cowan, N. J., Wilde, C. D., Chow, L. T., and Wefald, F. C., 1981, Structural variation among human β-tubulin genes. Proc. Natl. Acad. Sci. USA 78: 4877.PubMedGoogle Scholar
  25. Cowan, N. J., Dobner, P. R., Fuchs, E. V., and Cleveland, D. W., 1983, Expression of human α- tubulin genes: Interspecies conservation of 3’ untranslated regions, Mol. Cell. Biol. 3: 1738.PubMedGoogle Scholar
  26. Croall, D. E., and Morrison, M. R., 1980, Polysomal and non-polysomal messenge rRNA in neuroblastoma Cells: Lack of correlation between polyadenylation or initiation efficiency and messenger RNA location, J. Mol. Biol. 140: 549.PubMedGoogle Scholar
  27. Cronly-Dillon, J. R., and Perry, G. W., 1976, Tubulin synthesis in developing rat visual cortex, Nature 261: 581.PubMedGoogle Scholar
  28. Cronly-Dillon, J. R., and Perry, G. W., 1978, Tubulin synthesis in developing cerebral cortex of rat and kitten, J. Physiol. 287: 26.Google Scholar
  29. Cumming, R., Burgoyne, R. D., and Lytton, N. A., 1982, Differential immunocytochemical localisation of α-tubulin and 3-tubulin in cerebellum using monoclonal antibodies, Cell Biol. Int. Rep. 6: 1047.PubMedGoogle Scholar
  30. Cumming R., Burgoyne, R. D., and Lytton, N. A., 1983a, Axonal sub-populations in the central nervous system demonstrated using monoclonal antibodies against α-tubulin, Eur. J. Cell Biol. 31: 241.PubMedGoogle Scholar
  31. Cumming, R., Burgoyne, R. D., Lytton, N. A., and Gray, E. G., 1983b, Immunocytochemical evidence for tubulin in the presynaptic terminal of synaptosomes, Neurosci. Lett. 37: 215.PubMedGoogle Scholar
  32. Cumming, R., Burgoyne, R. D., and Lytton, N. A., 1984a, Immunocytochemical demonstration of a-tubulin modification during axonal maturation in the cerebellar cortex, J. Cell Biol. 98: 347.PubMedGoogle Scholar
  33. Cumming, R., Burgoyne, R. D., and Lytton, N. A., 1984b, Immunofluorescence distribution of a tubulin, p tubulin and microtubule-associated protein 2 during in vitro maturation of cerebellar granule Cell neurones, Neuroscience 12: 775.PubMedGoogle Scholar
  34. Dahl, J. L., and Weibel, V. J., 1979, Changes in tubulin heterogeneity during postnatal development of rat brain, Biochem. Biophys. Res. Commun. 86: 822.PubMedGoogle Scholar
  35. de Néchaud, B., Wolff, A., Jeantet, C., and Bourre, J-M., 1983, Characterization of tubulin in mouse brain myelin, J. Neurochem. 41: 1538.PubMedGoogle Scholar
  36. Denoulet, P., Edde, B., Jeantet, C., and Gros, F., 1982, Evolution of tubulin heterogeneity during mouse brain development, Biochimie 64: 165.PubMedGoogle Scholar
  37. Dustin, P., 1978, Microtubules, Springer-Verlag, New York.Google Scholar
  38. Eayrs, J. T., and Goodhead, B., 1959, Postnatal development of the cerebral cortex in the rat, J. Anat. 93: 385.PubMedGoogle Scholar
  39. Eddé, B., Jeantet, C., and Gros, F., 1981, One β-tubulin subunit accumulates during neurite outgrowth in mouse neuroblastoma Cells, Biochem. Biophys. Res. Comm. 103: 1035.PubMedGoogle Scholar
  40. Eddé, B., Portier, M.-M., Sahuquillo, C., Jeantet, C., and Gros, F., 1982, Changes in some cytoskeletal proteins during neuroblastoma Cell differentiation, Biochimie 64: 141.PubMedGoogle Scholar
  41. Eipper, B. A., 1972, Rat brain microtubule protein: purification and determination of covalently bound phosphate and carbohydrate, Proc. Natl. Acad. Sci. USA 69: 2283.PubMedGoogle Scholar
  42. Eipper, B. A., 1974a, Rat brain tubulin and protein kinase activity, J. Biol. Chem. 249: 1398.PubMedGoogle Scholar
  43. Eipper, B. A., 1974b, Properties of rat brain tubulin, J. Biol. Chem. 249: 1407.PubMedGoogle Scholar
  44. Elliott, E. M., Okayama, H., Sarangi, F., Henderson, G., and Ling, V., 1985, Differential expression of three α-tubulin genes in Chinese hamster ovary Cells, Mol. Cell Biol. 5: 236.PubMedGoogle Scholar
  45. Estridge, M., 1977, Polypeptides similar to the ol and (3 subunits of tubulin are exposed on the neuronal surface, Nature 268: 60.PubMedGoogle Scholar
  46. Feit, H., and Shelanski, M. L., 1975, Is tubulin a glycoprotein? Biochem. Biophys. Res. Commun. 66: 920.PubMedGoogle Scholar
  47. Feit, H., Slusarek, L., and Shelanski, M. L., 1971, Heterogeneity of tubulin subunits, Proc. Natl. Acad. Sci. USA 68: 2028.PubMedGoogle Scholar
  48. Feit, H., Kelly, P., and Cotman, C. W., 1977a, Identification of a protein related to tubulin in the postsynaptic density, Proc. Natl. Acad. Sci. USA 74: 1047.PubMedGoogle Scholar
  49. Feit, H., Neudeck, U., and Baskin, F., 1977b, Comparison of the isoelectric and molecular weight properties of tubulin subunits, J. Neurochem. 28: 697.PubMedGoogle Scholar
  50. Fellous, A., Francon, J., Virion, A., and Nunez, J., 1975, Microtubules and brain development, FEBS Lett. 57: 5.PubMedGoogle Scholar
  51. Fellous, A., Lennon, A. M., Francon, J., and Nunez, J., 1979, Thyroid hormones and neu- rotubule assembly in vitro during brain development, Eur. J. Biochem. 101: 365.PubMedGoogle Scholar
  52. Field, D. J., Collins, R. A., and Lee, J. C., 1984, Heterogeneity of vertebrate brain tubulins, Proc. Natl. Acad. Sci. USA 81: 4041.PubMedGoogle Scholar
  53. Forgue, S. T., and Dahl, J. L., 1979, Rat brain tubulin: subunit heterogeneity and phosphorylation, J. Neurochem. 32: 1015.PubMedGoogle Scholar
  54. Gard, D. L., and Kirschner, M. W., 1985, A polymer-dependent increase in phosphorylation of tubulin accompanies differentiation of a mouse neuroblastoma Cell line, J. Cell Biol. 100: 764.PubMedGoogle Scholar
  55. George, H. J., Misra, L., Field, D. J., and Lee, J. C., 1981, Polymorphism of brain tubulin, Biochemistry 20: 2402.PubMedGoogle Scholar
  56. Gilbert, J. M., Strocchi, P., Brown, B. A., and Marotta, C. A., 1981, Tubulin synthesis in rat forebrain: studies with free and membrane-bound polysomes, J. Neurochem. 36: 839.PubMedGoogle Scholar
  57. Ginzburg, I., de Baetselier, A., Walker, M. D., Behar, L., Lehrach, H., Frischauf, A. M., and Littauer, U. Z., 1980, Brain tubulin and actin cDNA sequences: Isolation of recombinant plasmids, Nucl. Acids Res. 8: 3553.Google Scholar
  58. Ginzburg, I., Behar, L., Givol, D., and Littauer, U. Z., 1981, The nucleotide sequence of rat a- tubulin: 3’-end characteristics, and evolutionary conservation, Nucl. Acids Res. 9: 2691.PubMedGoogle Scholar
  59. Ginzburg, I., Scherson, T., Giveon, D., Behar, L., and Littauer, U. Z., 1982, Modulation of mRNAs for microtubule-associated proteins during brain development, Proc. Natl. Acad. Sci. USA 79: 4892.PubMedGoogle Scholar
  60. Ginzburg, I., Rybak, S., Kimhi, Y., and Littauer, U. Z., 1983a, Biphasic regulation by dibutyryl cyclic AMP of tubulin and actin mRNA levels in neuroblastoma Cells, Proc. Natl. Acad. Sci. USA 80: 4243.PubMedGoogle Scholar
  61. Gonzales, L. W., and Geel, S. E., 1978, Quantitation and characterization of brain tubulin (Col- chicine-binding activity) in developing hypothyroid rats, J. Neurochem. 30: 237.PubMedGoogle Scholar
  62. Gozes, I., and Barnstable, C. J., 1982, Monoclonal antibodies that recognize discrete forms of tubulin, Proc. Natl. Acad. Sci. USA 79: 2579.PubMedGoogle Scholar
  63. Gozes, I., and Littauer, U. Z., 1978, Tubulin microheterogeneity increases with rat brain maturation, Nature 276: 411.PubMedGoogle Scholar
  64. Gozes, I., and Littauer, U. Z., 1979, The a-subunit of tubulin is preferentially associated with brain presynaptic membrane, FEBS Lett. 99: 86.PubMedGoogle Scholar
  65. Gozes, I., and Richter-Landsberg, C., 1978, Identification of tubulin associated with rat brain myelin, FEBS Lett. 95: 169.PubMedGoogle Scholar
  66. Gozes, I., and Sweadner, K. J., 1981, Multiple tubulin forms are expressed by a single neurone, Nature 294: 477.PubMedGoogle Scholar
  67. Gozes, I., Saya, D., and Littauer, U. Z., 1979, Tubulin microheterogeneity in neuroblastoma and glioma Cell lines differs from that of the brain, Brain Res. 171: 171.PubMedGoogle Scholar
  68. Gozes, I., de Baetselier, A., and Littauer, U. Z., 1980, Translation in vitro of rat brain mRNA coding for a variety of tubulin forms, Eur. J. Biochem. 103: 13.Google Scholar
  69. Griffin, W. S. T., and Morrison, M. R., 1985, In situ hybridization—visualization and quantitation of genetic expression in brain, Peptides 6 (Suppl. 2): 89.PubMedGoogle Scholar
  70. Griffin, W. S. T., Alejos, M., Nilaver, G., and Morrison, M. R., 1983, Brain protein and messenger RNA identification in the same Cell, Brain Res. Bull. 10: 597.PubMedGoogle Scholar
  71. Griffin, W. S. T., Alejos, M., Cox, E. J., and Morrison, M. R., 1985, The differential distribution of beta tubulin mRNAs in individual mammalian brain Cells, J. Cell. Biochem. 27: 205.PubMedGoogle Scholar
  72. Gwo-Shu Lee, M., Lewis, S. A., Wilde, C. D., and Cowan, N. J., 1983, Evolutionary history of a multigene family: An expressed human β-tubulin gene and three processed pseudogenes, Cell 33: 477.PubMedGoogle Scholar
  73. Hajos, F., and Rostomian, M. A., 1984, Localization of a-tubulin immunoreactivity to cerebellar Bergmann glia with the TU 01 antibody, Histochemistry 81: 297.PubMedGoogle Scholar
  74. Hall, J. L., Dudley, L., Dobner, P. R., Lewis, S. A., and Cowan, N. J., 1983, Identification of two human β-tubulin isotypes, Mol. Cell. Biol. 3: 854.PubMedGoogle Scholar
  75. Havercroft, J. C., and Cleveland, D. W., 1984, Programmed expression of β-tubulin genes during development and differentiation of the chicken, J. Cell Biol. 99: 1927.PubMedGoogle Scholar
  76. Hawkins, D. J., and Browning, E. T., 1982, Tubulin adenosine diphosphate ribosylation is catalyzed by cholera toxin, Biochemistry 21: 4474.PubMedGoogle Scholar
  77. Hicks, S. P., and D’Amato, C. J., 1968, Cell migration to the isocortex in the rat, Anat. Rec. 160: 619.PubMedGoogle Scholar
  78. Ilaria, R., Wines, D., Pardue, S., Jamison, S., Ojeda, S., Snider, J., and Morrison, M. R., 1985, A rapid microprocedure for isolating RNA from multiple samples of human and rat brain, J. Neurosci. Meth. 15: 165.Google Scholar
  79. Jacobson, M., 1978, Developmental Neurobiology, Plenum Press, New York.Google Scholar
  80. Kilmartin, J. V., Wright, B., and Milstein, C., 1982, Rat monoclonal antitubulin antibodies de¬rived by using a new nonsecreting rat Cell line, J. Cell Biol. 93: 576.PubMedGoogle Scholar
  81. Krauhs, E., Little, M., Kempf, T., Hofer-Warbinek, R., Ade, W., and Ponstingl, H., 1981, Complete amino acid sequence of p-tubulin from porcine brain, Proc. Natl. Acad. Sci. USA 78: 4156.PubMedGoogle Scholar
  82. Lee, J. C., Frigon, R. P., and Timasheff, S. N., 1973, The chemical characterization of calf brain microtubule protein subunits, J. Biol. Chem. 248: 7253.PubMedGoogle Scholar
  83. Lemischka, I., and Sharp, P. A., 1982, The sequences of an expressed rat α-tubulin gene and a pseudogene with an inserted repetitive element, Nature 300: 330.PubMedGoogle Scholar
  84. Lemischka, I. R., Farmer, S., Racaniello, V. R., and Sharp, P. A., 1981, Nucleotide sequence and evolution of a mammalian α-tubulin messenger RNA, J. Mol. Biol. 151: 101.PubMedGoogle Scholar
  85. L’Hernault, S. W., and Rosenbaum, J. L., 1985, Chlamydomonas alpha-tubulin is posttranslationally modified by acetylation on the epsilon amino group of a lysine, Biochemistry 24: 473.PubMedGoogle Scholar
  86. Littauer, U. Z., and Ginzburg, I., 1985, Expression of microtubule proteins in brain, in: Gene Expression in Brain ( C. Zomzely-Neurath and W. A. Walker, eds.), pp. 125–156, Wiley, New York.Google Scholar
  87. Littauer, U. Z., Schmitt, H., and Gozes, I., 1976, Properties and synthesis of tubulin in neuroblastoma Cells, J. Natl. Cancer Inst. 57: 647.PubMedGoogle Scholar
  88. Little, M., 1979, Identification of a second 0 chain in pig brain tubulin, FEES. Lett. 108: 283.Google Scholar
  89. Little, M., and Luduena, R. F., 1985, Structural differences between brain β1- and β2-tubulins: Implications for microtubule assembly and colchicine binding, EMBO J. 4: 51.PubMedGoogle Scholar
  90. Lopata, M. A., Havercroft, J. C., Chow, L. T., and Cleveland, D. W., 1983, Four unique genes required for βtubulin expression in vertebrates, Cell 32: 713.PubMedGoogle Scholar
  91. Luduena, R. F., and Woodward, D. O., 1973, Isolation and partial characterization of a- and tubulin from outer doublets of sea-urchin sperm and microtubules of chick embryo brain, Proc. Natl Acad. Sci. USA 70: 3594.PubMedGoogle Scholar
  92. Mareck, A., Fellous, A., Francon, J., and Nunez, J., 1980, Changes in the composition and activity of microtubule-associated proteins during brain development, Nature 284: 353.PubMedGoogle Scholar
  93. Marotta, C. A., Harris, J. L., and Gilbert, J. M., 1978, Characterization of multiple forms of brain tubulin subunits, J. Neurochem. 30: 1431.PubMedGoogle Scholar
  94. Marotta, C. A., Strocchi, P., and Gilbert, J. M., 1979a, Subunit structure of synaptosomal tubulin, Brain Res. 167: 93.PubMedGoogle Scholar
  95. Marotta, C. A., Strocchi, P., and Gilbert, J. M., 1979b, Biosynthesis of heterogeneous forms of mammalian brain tubulin subunits by multiple messenger RNAs, J. Neurochem. 33: 231.PubMedGoogle Scholar
  96. Morrison, M. R., and Griffin, W. S. T., 1981, The isolation and in vitro translation of undegraded messenger RNAs from human postmortem brain, Anal. Biochem. 113: 318.PubMedGoogle Scholar
  97. Morrison, M. R., and Griffin, W. S. T., 1985a, Molecular biology of the mammalian brain, in: Gene Expression in Brain ( C. Zomzely-Neurath and W. A. Walker, eds.), pp. 57–98, Wiley, New York.Google Scholar
  98. Morrison, M. R., and Griffin, W. S. T., 1985a, Molecular biology of the mammalian brain, in: Gene Expression in Brain ( C. Zomzely-Neurath and W. A. Walker, eds.), pp. 57–98, Wiley, New York.Google Scholar
  99. Morrison, M. R., Brodeur, R., Pardue, S., Baskin, F., Hall, C. L., and Rosenberg, R. N., 1979, Differences in the distribution of poly(A) size classes in individual messenger RNAs from neuroblastoma Cells, J. Biol. Chem. 254: 7675.PubMedGoogle Scholar
  100. Morrison, M. R., Pardue, S., Prashad, N., and Brodeur, R., 1980, Relative increase in polysomal mRNA for R1 cAMP binding protein in neuroblastoma Cells treated with 1,N6-dibutyryl- adenosine 3’,-5’-phosphate, Eur. J. Biochem. 106: 463.PubMedGoogle Scholar
  101. Morrison, M. R., Pardue, S., and Griffin, W. S. T., 1981, Developmental alterations in the levels of translationally active messenger RNAs in the postnatal rat cerebellum, J. Biol. Chem. 256: 3350.Google Scholar
  102. Morrison, M. R., Pardue, S., and Griffin, W. S. T., 1983, Altered expression of different tubulin electrophoretic variants during human cortex development, J. Neurogenet. 1: 105.PubMedGoogle Scholar
  103. Nath, J., and Flavin, M., 1978, A structural difference between cytoplasmic and membrane- bound tubulin of brain, FEBS Lett. 95: 335.PubMedGoogle Scholar
  104. Nelles, L. P., and Bamburg, J. R., 1979, Comparative peptide mapping and isoelectric focusing of isolated subunits from chick embryo brain tubulin, J. Neurochem. 32: 477.PubMedGoogle Scholar
  105. Olmsted, J. B., and Borisy, G. G., 1973, Microtubules, Ann. Rev. Biochem. 42: 507.PubMedGoogle Scholar
  106. Olmsted, J., Witman, G., Carlson, K., and Rosenbaum, J. L., 1971, Comparison of the micro-tubule proteins of neuroblastoma Cells, brain, and chlamydomonas flagella, Proc. Natl. Acad. Sci. USA 68: 2273.PubMedGoogle Scholar
  107. Perry, G. W., and Cronly-Dillon, J. R., 1978, Tubulin synthesis during a critical period in visual cortex development, Brain Res. 142: 374.PubMedGoogle Scholar
  108. Ponstingl, H., Krauhs, E., Little, M., and Kempf, T., 1981, Complete amino acid sequence of α- tubulin from porcine brain, Proc. Natl. Acad. Sci. USA 78: 2757.PubMedGoogle Scholar
  109. Prashad, N., and Rosenberg, R. N., 1978, Induction of cyclic AMP binding proteins by dibutyryl cyclic AMP in mouse neuroblastoma Cells, Biochim. Biophys. Acta 539: 459.PubMedGoogle Scholar
  110. Raff, E. C., 1984, Genetics of microtubule systems, J. Cell Biol. 99: 1.PubMedGoogle Scholar
  111. Raybin, D., and Flavin, M., 1975, An enzyme tyrosylating a-tubulin and its role in microtubule assembly, Biochem. Biophys. Res. Commun. 65: 1088.PubMedGoogle Scholar
  112. Reddington, M., Tan, L. P., and Lagnado, J. R., 1976, The phosphorylation of brain micro- tubular proteins in situ and in vitro, J. Neurochem. 27: 1229.PubMedGoogle Scholar
  113. Rodriguez, J. A., and Borisy, G. G., 1978, Modification of the C-terminus of brain tubulin during development, Biochem. Biophys. Res. Commun. 83: 579.PubMedGoogle Scholar
  114. Saborio, J. L., Palmer, E., and Meza, I., 1978, In vivo and in vitro synthesis of rat brain α- and β- tubulins, Exp. Cell Res. 114:365.PubMedGoogle Scholar
  115. Sato, M., Yoshida, Y., and Takahashi, Y., 1978, In vitro biosynthesis of tubulin on total, free and membrane-bound polysomes from the developing rat brain, J. Neurochem. 31: 1361.PubMedGoogle Scholar
  116. Schmitt, H., 1976, Control of tubulin and actin synthesis and assembly during differentiation of neuroblastoma Cells, Brain Res. 115: 165.PubMedGoogle Scholar
  117. Schmitt, H., Gozes, I., and Littauer, U. Z., 1977, Decrease in levels and rates of synthesis of tubulin and actin in developing rat brain, Brain Res. 121: 327.PubMedGoogle Scholar
  118. Soifer, D., and Czosnek, H., 1980, The possible origin of neuronal plasma membrane tubulin, in: Microtubules and Microtubule Inhibitors ( M. De Brabander and J. De Mey, eds.) pp. 429–447, Elsevier/North Holland, Amsterdam.Google Scholar
  119. Solomon, F., Gysin, R., Rentsch, M., and Monard, D., 1976, Purification of tubulin from neuroblastoma Cells: Absence of covalently bound phosphate in tubulin from normal and morphologically differentiated Cells, FEBS Lett. 63: 316.PubMedGoogle Scholar
  120. Strocchi, P., Marotta, C. A., Bonventre, J., and Gilbert, J. M., 1981, The subunit composition of cerebellar tubulin: Evidence for multiple β-tubulin messenger mRNAs, Brain Res. 211: 206.PubMedGoogle Scholar
  121. Stromska, D. P., 1982, Isoelectric focusing of tubulin in a new type of charge-balanced agarose, Anal. Biochem. 119: 387.PubMedGoogle Scholar
  122. Sullivan, K. F., and Cleveland, D. W., 1984, Sequence of a highly divergent β-tubulin gene reveals regional heterogeneity in the β-tubulin polypeptide, J. Cell Biol. 99: 1754.PubMedGoogle Scholar
  123. Sullivan, K. F., and Wilson, L., 1984, Developmental and biochemical analysis of chick brain tubulin heterogeneity, J. Neurochem. 42: 1363.PubMedGoogle Scholar
  124. Tashiro, T., and Komiya, Y., 1983, Subunit composition specific to axonally transported tubulin, Neuroscience 9: 943.PubMedGoogle Scholar
  125. Valenzuela, P., Quiroga, M., Zaldivar, J., Rutter, W. J., Kirschner, M. W., and Cleveland, D. W., 1981, Nucleotide and corresponding amino acid sequences encoded by a and p tubulin mRNAs, Nature 289: 650.PubMedGoogle Scholar
  126. Viklicky, V., Draber, P., Hasek, J., and Bartek, J., 1982, Production and characterization of a monoclonal antitubulin antibody, Cell Biol. Int. Rep. 6: 725.PubMedGoogle Scholar
  127. von Hungen, K., Chin, R. C., and Baxter, C. F., 1981, Brain tubulin microheterogeneity in the mouse during development and aging, J. Neurochem. 37: 511.Google Scholar
  128. Waeheldt, T. V., and Malokta, J., 1980, Comparative electrophoretic study of the wolfgram proteins in myelin from several mammalia, Brain Res. 189: 582.Google Scholar
  129. Wehland, J., Willingham, M. C., and Sandoval, I. V., 1983, A rat monoclonal antibody reacting specifically with the tyrosylated form of α-tubulin. 1. Biochemical characterization, effects on microtubule polymerization in vitro, and microtubule polymerization and organization in vivo, J. Cell Biol. 97: 1467.PubMedGoogle Scholar
  130. Wehland, J., Schröder, H. C., and Weber, K., 1984, Amino acid sequence requirements in the epitope recognized by the α-tubulin-specific rat monoclonal antibody YL1/2, EMBO J. 3: 1295.PubMedGoogle Scholar
  131. Wilde, C. D., Chow, L. T., Wefald, F. C., and Cowan, N. J., 1982a, Structure of two human a- tubulin genes, Proc. Natl. Acad. Sci. USA 79: 96.PubMedGoogle Scholar
  132. Wilde, C. D., Crowther, C. E., and Cowan, N. J., 1982b, Isolation of a multigene family containing human a-tubulin sequences, J. Mol. Biol. 155: 533.PubMedGoogle Scholar
  133. Wilde, C. D., Crowther, C. E., Cripe, T. P., Lee, M. G.-S., and Cowan, N. J., 1982c, Evidence that a human p-tubulin pseudogene is derived from its corresponding mRNA, Nature 297: 83.PubMedGoogle Scholar
  134. Witman, G. B., Cleveland, D. W., Weingarten, M. D., and Kirschner, M. W., 1976, Tubulin requires tau for growth onto microtubule initiating sites, Proc. Natl. Acad. Sci. USA 73: 4070.PubMedGoogle Scholar
  135. Wolff, A., Denoulet, P., and Jeantet, C., 1982, High level of tubulin microheterogeneity in the mouse brain, Neurosci. Lett. 31: 323.PubMedGoogle Scholar
  136. Zagon, I. S., and McLaughlin, P. J., 1979, Morphological identification and biochemical characterization of isolated brain Cell nuclei from the developing rat cerebellum, Brain Res. 170: 443.PubMedGoogle Scholar
  137. Zisapel, N., Levi, M., and Gozes, I., 1980, Tubulin: An integral protein of mammalian synaptic vesicle membranes, J. Neurochem. 34: 26.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Marcelle R. Morrison
    • 1
  • W. Sue T. Griffin
    • 2
  1. 1.Department of NeurologyUniversity of Texas Health Science Center at DallasDallasUSA
  2. 2.Department of Cell BiologyUniversity of Texas Health Science Center at DallasDallasUSA

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