Visualization of Cisplatin-DNA and Carboplatin-DNA Adducts in Tissues and Cultured Cells
We are interested in the contribution of cisplatin- (cis-diamminedichloroplatinum, cDDP) and carboplatin- (cis-diammine (1,1-cyclobutanedicarboxylato)-platinum(II), CBDCA) induced DNA modifications to the cytotoxic and other biological effects of these drugs. An antiserum against in vitro cDDP modified DNA was therefore applied in an immunocytochemical double peroxidase assay. It proved to be possible to visualize cDDP-DNA and CBDCA-DNA binding both in tissues and cultured cells (1).
KeywordsNuclear Staining Immunocytochemical Assay Buccal Epithelial High Staining Intensity A1847 Tumour Cell
Unable to display preview. Download preview PDF.
- 1.Terheggen P.M.A.B., Scherer E., Jenner A.A.J., Begg A.C., Den Engelse L. Fifth NCI/EORTC symposium on new drugs in cancer therapy, Amsterdam, The Netherlands, 1986. Abstr. 1.34.Google Scholar