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Visualization of Cisplatin-DNA and Carboplatin-DNA Adducts in Tissues and Cultured Cells

  • P. Terheggen
  • A. C. Begg
  • B. Floot
  • J. Emondt
  • L. Den Engelse
Part of the Developments in Oncology book series (DION, volume 54)

Abstract

We are interested in the contribution of cisplatin- (cis-diamminedichloroplatinum, cDDP) and carboplatin- (cis-diammine (1,1-cyclobutanedicarboxylato)-platinum(II), CBDCA) induced DNA modifications to the cytotoxic and other biological effects of these drugs. An antiserum against in vitro cDDP modified DNA was therefore applied in an immunocytochemical double peroxidase assay. It proved to be possible to visualize cDDP-DNA and CBDCA-DNA binding both in tissues and cultured cells (1).

Keywords

Nuclear Staining Immunocytochemical Assay Buccal Epithelial High Staining Intensity A1847 Tumour Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Terheggen P.M.A.B., Scherer E., Jenner A.A.J., Begg A.C., Den Engelse L. Fifth NCI/EORTC symposium on new drugs in cancer therapy, Amsterdam, The Netherlands, 1986. Abstr. 1.34.Google Scholar
  2. 2.
    Poirier M.C., Lippard S.J., Zwelling L.A., Ushay H.M., Kerringer D., Thill C.C., Santella R.M., Grundberger D., Yuspa S.H. Proc. Natl. Acad. Sei. USA. 79: 6443–6447, 1982.CrossRefGoogle Scholar
  3. 3.
    Heyting C., Van der Laken C.J., Van Raamsdonk W., Pool C.W. Cancer Res. 43: 2935–2941, 1983.PubMedGoogle Scholar
  4. 4.
    Muysken-Schoen M.A., Baan R.A., Lohman P.H.M. Carcinogenesis 6, 999–1004, 1985.PubMedCrossRefGoogle Scholar
  5. 5.
    Knox R.J., Friedlos F., Lydall D.A., Roberts J.J. Cancer Res. 46: 1972–1979, 1986.PubMedGoogle Scholar
  6. 6.
    Stewart F., Bohlken S., Begg A., Bartelink H. Int. J. Radiat. Oncol. Biol. Phys. 12: 927–933, 1986.PubMedCrossRefGoogle Scholar

Copyright information

© Martinus Nijhoff Publishing, Boston 1988

Authors and Affiliations

  • P. Terheggen
  • A. C. Begg
  • B. Floot
  • J. Emondt
  • L. Den Engelse

There are no affiliations available

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