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Tumor targeting with monoclonal antibody B72.3: Experimental and clinical results

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Cancer Imaging with Radiolabeled Antibodies

Part of the book series: Cancer Treatment and Research ((CTAR,volume 51))

Abstract

Several monoclonal antibodies (Mabs) are currently the subject of active investigation in the diagnostic imaging of human carcinomas [1–11]. While the diagnostic targeting of a primary or metastatic carcinoma lesion with a Mab is an end unto itself, it should also be considered as a first step toward the use of that Mab in tumor therapy. This is particularly true in those studies in which biopsies of tumor and normal tissue are obtained subsequent to radiolabeled Mab administration. Dosimetry calculations can be made by: 1) analysis of these specimens for counts per minute (cpm) of Mab bound per gram of tumor versus cpm of Mab per gram of normal tissues and 2) analysis of the pharmacokinetics of Mab clearance from sera, to estimate the potential therapeutic efficacy of administering higher doses of the same radiolabeled Mab or for administering the same Mab coupled to a more efficient killer isotope. Alternatively, one gains information regarding the use of that Mab in a patient, either coupled to a drug or toxin, or the use of native Mab to elicit effector cell mechanisms.

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References

  1. Epenetos, A.A., Snook, D., Durbin, H., Johnson, P.M., and Taylor-Papadimitriou, (1986) Limitations of radiolabeled monoclonal antibodies for localization of human neoplasms. Cancer Res. 46:3183–3191.

    PubMed  CAS  Google Scholar 

  2. Beatty, J.D., Duda, R.B., Williams, L.E., Sheibani, K., Paxton, R.J., Beatty, B.G., Philben, V.J., Werner, J.L., Shively, J.E., Vlahos, W.G., Kokal, W.A., Riihimaki, D.U., Terz, J.J., and Wagman, L.D. (1986) Preoperative imaging of colorectal carcinoma with 111Inlabeled anticarcinoembryonic antigen monoclonal antibody. Cancer Res. 46:6494–6502.

    PubMed  CAS  Google Scholar 

  3. Allum, W.H., MacDonald, F., Anderson, P., and Fielding, J.W.L. (1986) Localisation of gastrointestinal cancer with a 131I-labeled monoclonal antibody to CEA. Br. J. Cancer 53: 203–210.

    Article  PubMed  CAS  Google Scholar 

  4. Pateisky, N., Philipp, K., Skodler, W.D., Czerwenka, K., Hamilton, G., and Burchell, J. (1985) Radioimmunodetection in patients with suspected ovarian cancer. J. Nucl. Med. 26: 1369–1375.

    PubMed  Google Scholar 

  5. Wahl, R.L., Khazaeli, M.B., LoBuglio, A.F., Patillo, R.A., Tuscan, M.J., and Beierwaltes, W.H. (1987) Radioimmunoscintigraphic detection of occult gestational choriocarcinoma. Am. J. Obstet. Gynecol. 156:108–111.

    PubMed  CAS  Google Scholar 

  6. Mach, J.-P., Buchegger, F., Forni, M., Ritschard, J., Berchi, C, Lumbroso, J.-D., Schreyer, M., Girardet, C, Accola, R.S., and Carrel, S. (1981) Use of radiolabeled monoclonal anti-CEA antibodies for the detection of human carcinomas by external photo-scanning and tomoscintigraphy. Immunol. Today 2:239–249.

    Article  Google Scholar 

  7. Moldofsky, P.J., Powe, J., Mulhern, C.B., Jr., Hammond, N., Sears, H.F., Gatenby, R.A., Steplewski, Z., and Koprowski, H. (1983) Metastatic colon carcinoma detected with radiolabeled F(ab’)2 monoclonal antibody fragments. Radiology 149:549–555.

    PubMed  CAS  Google Scholar 

  8. Mach, J.-P., Chatal, J.-F., Lumbroso, J.-D., Buchegger, F., Forni, M., Ritschard, J., Berche C, Douillard, J.-Y., Carrel, S., Heryln, M., Steplewski, Z., and Koprowski, H. (1983) Tumor localization in patients by radiolabeled monoclonal antibodies against colon carcinoma. Cancer Res. 43:5593–5600.

    PubMed  CAS  Google Scholar 

  9. Chatal, J.-F., Saccavini, J.-C, Fumoleau, P., Doulliard, J.-Y., Curtet, C, Kremer, M., LeMevel, B., and Koprowski, H. (1984) Immunoscintigraphy of colon carcinoma. J. Nucl. Med. 25:307–314.

    PubMed  CAS  Google Scholar 

  10. Armitage, N.C., Perkins, A.C., Pimm, M.V., Farrands, P.A., Baldwin, R.W., and Hardcastle, J.D. (1984) The localization of an anti-tumour monoclonal antibody (791T/36) in gastrointestinal tumours. J. Surg. 71:407–412.

    CAS  Google Scholar 

  11. Goldenberg, D.M., Kim, E.E., Bennett, S.J., Nelson, M.O., and DeLand, F.H. (1983) Carcinoembryonic antigen radioimmunodetection in the evaluation of colorectal cancer and in the detection of occult neoplasms. Gastroenterology 84:524–532.

    PubMed  CAS  Google Scholar 

  12. Schlom, J. (1986) Basic principles and applications of monoclonal antibodies in the management of carcinomas: The Richard and Hinda Rosenthal Foundation Award Lecture. Cancer Res. 46:3225–3238.

    PubMed  CAS  Google Scholar 

  13. Colcher, D., Horan Hand, P., Nuti, M., and Schlom, J. (1981) A spectrum of monoclonal antibodies reactive with human mammary tumor cells. Proc. Natl. Acad. Sci. USA 78: 3199–3203.

    Article  PubMed  CAS  Google Scholar 

  14. Johnson, V.G., Schlom, J., Paterson, A.J., Bennett, J., Magnani, J.L., and Colcher, D. (1986) Analysis of a human tumor-associated glycoprotein (TAG-72) using monoclonal antibody B72.3. Cancer Res. 46:850–857.

    PubMed  Google Scholar 

  15. Paterson, A.J., Schlom, J., Sears, H.F., Bennett, J., and Colcher, D. (1986) A radioimmunoassay for the detection of a human tumor-associated glycoprotein (TAG-72) using monoclonal antibody B72.3. Int. J. Cancer 37:659–666.

    Article  PubMed  CAS  Google Scholar 

  16. Klug, T.L., Sattler, M.A., Colcher, D., and Schlom, J. (1986) Monoclonal antibody immunoradiometric assay for an antigenic determinant (CA 72) on a novel pancarcinoma antigen (TAG-72). Int. J. Cancer 38:661–669.

    Article  PubMed  CAS  Google Scholar 

  17. Thor, A., Ohuchi, N., Szpak, CA., Johnston, W.W., and Schlom, J. (1986) The distribution of oncofetal antigen TAG-72 defined by monoclonal antibody B72.3. Cancer Res. 46: 3118–3124.

    PubMed  CAS  Google Scholar 

  18. Stramignoni, D., Bowen, R., Atkinson, B., and Schlom, J. (1983) Differential reactivity of monoclonal antibodies with human colon adenocarcinomas and adenomas. Int. J. Cancer 31:543–552.

    Article  PubMed  CAS  Google Scholar 

  19. Nuti, M., Teramoto, Y.A., Mariani-Costantini, R., Horan Hand, P., Colcher, D., and Schlom, J. (1982) A monoclonal antibody (B72.3) defines patterns of distribution of a novel tumor associated antigen in human mammary carcinoma cell populations. Int. J. Cancer 29: 539–545.

    Article  PubMed  CAS  Google Scholar 

  20. Nuti, M., Mottolese, M. Viora, Donnorso, R.P., Schlom, J., and Natali, P.G. (1986) Use of monoclonal antibodies to human breast tumor associated antigens in fine needle aspirate cytology. Int. J. Cancer 37:493–498.

    Article  PubMed  CAS  Google Scholar 

  21. Johnston, W.W., Szpak, CA., Lottich, S.C, Thor, A., and Schlom, J. (1986) Use of a monoclonal antibody (B72.3) as a novel immunohistochemical adjunct for the diagnosis of carcinomas in fine needle aspiration biopsy specimens. Human Pathol. 17:501–513.

    Article  CAS  Google Scholar 

  22. Szpak, CA., Johnston, W.W., Roggli, V., Kolbeck, J., Lottich, S.C, Vollmer, R., Thor, A., and Schlom, J. (1986) The diagnostic distinction between malignant mesothelioma of the pleura and adenocarcinoma of the lung as defined by a monoclonal antibody (B72.3). Am. J. Pathol. 122:252–260.

    PubMed  CAS  Google Scholar 

  23. Thor, A., Gorstein, F., Ohuchi, N., Szpak, CA., Johnston, W.W., and Schlom, J. (1986) Tumor-associated glycoprotein (TAG-72) in ovarian carcinomas defined by monoclonal antibody B72.3. J. Natl. Cancer Inst. 76:995–1006.

    PubMed  CAS  Google Scholar 

  24. Thor, A., Viglione, M.J., Muraro, R., Ohuchi, N., Schlom, J., and Gorstein, F. (1987) Monoclonal antibody B72.3 reactivity with human endometrium: A study of normal and malignant tissues. Int. J. Gynecol. Pathol. 6:235–247.

    Article  PubMed  CAS  Google Scholar 

  25. Szpak, C.A., Johnston, W.W., Lottich, S.C, Kufe, D., Thor, A., and Schlom, J. (1984) Patterns of reactivity of four novel monoclonal antibodies (B72.3, DF3, B1.1, B6.2) with cells in human malignant and benign effusions: A highly selective recognition of adenocarcinoma over the neoplasms and mesthothelium. Acta Cytol. 28:356–357.

    PubMed  CAS  Google Scholar 

  26. Lan, M.S., Bast, R.C., Jr., Colnaghi, M.I., Knapp, R.C, Colcher, D., Schlom, J., and Metzgar, R.S. (1987) Co-expression of human cancer-associated epitopes on mucin molecules. Int. J. Cancer 39:68–72.

    Article  PubMed  CAS  Google Scholar 

  27. Johnston, W.W., Szpak, CA., Lottich, S.C, Thor, A., and Schlom, J. (1985) Use of monoclonal antibody (B72.3) as an immunocytochemical adjunct to diagnosis of adenocarcinoma in human effusions. Cancer Res. 45:1894–1900.

    PubMed  CAS  Google Scholar 

  28. Ness, M.J., Pour, P.M., Tempero, M.A., and Linder, J. (1988) Immunohistochemistry with monoclonal antibody B72.3 as an adjunct in the cytologic diagnosis of pancreatic carcinoma. Modern Pathology 1:279–283.

    PubMed  CAS  Google Scholar 

  29. Martin, S.E., Moshiri, S., Thor, A., Vilasi, V., Chu, E.W., and Schlom, J. (1986) Identification of adenocarcinoma in cytospin preparations of effusions using monoclonal antibody B72.3. Am. J. Clin. Pathol. 86:10–18.

    PubMed  CAS  Google Scholar 

  30. Colcher, D., Keenan, A.M., Larson, S.M., and Schlom, J. (1984) Prolonged binding of a radiolabeled monoclonal antibody (B72.3) used for the in-situ radioimmunodetection of human colon carcinoma xenografts. Cancer Res. 44:5744–5751.

    PubMed  CAS  Google Scholar 

  31. Keenan, A.M., Colcher, D., Larson, S.M., and Schlom, J. (1984) Radioimmunoscintigraphy of human colon cancer xenografts in mice with radioiodinated monoclonal antibody B72.3. J. Nucl. Med. 25:1197–1203.

    PubMed  CAS  Google Scholar 

  32. Esteban, J.M., Schlom, J., Mornex, F., and Colcher, D. (1987) Radioimmunotherapy of athymic mice bearing human colon carcinoma with monoclonal antibody B72.3: Histological and autoradiographic study of effects on tumors and normal organs. Eur. J. Cancer Clin. Oncol. 23:643–655.

    Article  PubMed  CAS  Google Scholar 

  33. Esteban, J.M., Colcher, D., Sugarbaker, P., Carrasquillo, J.A., Bryant, G., Thor, A., Reynolds, J.C., Larson, S.M., and Schlom, J. (1987) Quantitative and qualitative aspects of radiolocalization in colon cancer patients of intravenously administered Mab B72.3. Int. J. Cancer 39:50–59.

    Article  PubMed  CAS  Google Scholar 

  34. Colcher, D., Esteban, J.M., Carrasquillo, J.A., Sugarbaker, P., Reynolds, J.C, Bryant, G., Larson, S.M., and Schlom, J. (1987) Quantitative analyses of selective radiolabeled monoclonal antibody localization in metastatic lesions of colorectal cancer patients. Cancer Res. 47:1185–1189.

    PubMed  CAS  Google Scholar 

  35. Colcher, D., Esteban, J., Carrasquillo, J.A., Sugarbaker, P., Reynolds, J.C, Bryant, G., Larson, S.M., and Schlom, J. (1987) Complementation of intracavitary and intravenous administration of a monoclonal antibody (B72.3) in patients with carcinoma. Cancer Res. 47: 4218–4224.

    PubMed  CAS  Google Scholar 

  36. Larson, S.M., Carrasquillo, J.A., Colcher, D., Reynolds, J.C, Sugarbaker, P., and Schlom, J. (1986) Considerations for radiotherapy of pseudomyxoma peritonei with i.p. 1–131 B72.3, a monoclonal antibody. J. Nucl. Med. 27:1021–1022.

    Google Scholar 

  37. Whittle, N., Adair, J., Lloyd, C, Jenkins, L., Schlom, J., Roubitschek, A., Colcher, D., and Bodmer, M. (1987) Expression in COS cells of a mouse-human chimaeric B72.3 antibody. Protein Engineering 1:499–505.

    Article  PubMed  CAS  Google Scholar 

  38. Roselli, M., Schlom, J., Gansow, O.A., Raubitschek, A., Mirzadeh, S., Brechbiel, M.W., and Colcher, D. (1989) Comparative biodistributions of Yttrium- and Indium-labeled monoclonal antibody B72.3 in athymic mice bearing human colon carcinoma xenografts. J. Nucl. Med. 30:672–682.

    PubMed  CAS  Google Scholar 

  39. Ohuchi, N., Simpson, J., Colcher, D., and Schlom, J. (1987) Complementation of anti-CEA and anti-TAG-72 monoclonal antibodies in reactivity to human gastric adenocarcinomas. Int. J. Cancer 40:726–733.

    Article  PubMed  CAS  Google Scholar 

  40. Muraro, R., Wunderlich, D., Thor, A., Lundy, J., Noguchi, P., Cunningham, R., and Schlom, J. (1985) Definition by monoclonal antibodies of a repertoire on carcinoembryonic antigen differentially expressed in human colon carcinomas versus normal adult tissues. Cancer Res. 45:5769–5780.

    PubMed  CAS  Google Scholar 

  41. Greiner, J.W., Guadagni, F., Noguchi, P., Pestka, S., Colcher, D., Fisher, P.B., and Schlom, J. (1987) Recombinant interferon enhances monoclonal antibody-targeting of carcinoma lesions in vivo. Science 235:895–898.

    Article  PubMed  CAS  Google Scholar 

  42. Greiner, J.W., Horan Hand, P., Colcher, D., Weeks, M., Thor, A., Noguchi, P., Pestka, S., and Schlom, J. (1987) Modulation of human tumor-associated antigen expression. J. Lab. Clin. Med. 109:244–261.

    PubMed  CAS  Google Scholar 

  43. Greiner, J.W., Horan Hand, P., Noguchi, P., Fisher, P.B., Pestka, S., and Schlom, J. (1984) Enhanced expression of surface tumor-associated antigens on human breast and colon tumors cells after recombinant human leukocyte alpha-interferon treatment. Cancer Res. 44: 3208–3214.

    PubMed  CAS  Google Scholar 

  44. Muraro, R., Kuroki, W., Wunderlich, D., Poole, D.J., Colcher, D., Thor, A., Greiner, J.W., Simpson, J.F., Molinolo, A., Noguchi, P., and Schlom, J. (1988) Generation and characterization of B72.3 second generation monoclonal antibodies reactive with the Tumorassociated Glycoprotein 72 antigen. Cancer Res. 48:4588–4596.

    PubMed  CAS  Google Scholar 

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Authors
  1. J. Schlom
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  2. D. Colcher
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  3. K. Siler
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  4. A. Thor
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  5. G. Bryant
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  6. W. W. Johnston
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  7. C. A. Szpak
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  8. P. Sugarbaker
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  9. J. A. Carrasquillo
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  10. J. C. Reynolds
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  11. A. M. Keenan
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  12. S. M. Larson
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Editor information

Editors and Affiliations

  1. Center for Molecular Medicine and Immunology, University of Medicine and Dentistry of New Jersey, 1 Bruce St., Newark, New Jersey, 07103, USA

    David M. Goldenberg Sc.D., M.D.

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© 1990 Kluwer Academic Publishers

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Schlom, J. et al. (1990). Tumor targeting with monoclonal antibody B72.3: Experimental and clinical results. In: Goldenberg, D.M. (eds) Cancer Imaging with Radiolabeled Antibodies. Cancer Treatment and Research, vol 51. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1497-4_16

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  • DOI: https://doi.org/10.1007/978-1-4613-1497-4_16

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-8805-3

  • Online ISBN: 978-1-4613-1497-4

  • eBook Packages: Springer Book Archive

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