• S. S. Deshpande


Qualitative and quantitative analytical techniques are of tremendous importance in all fields and disciplines of life sciences for the detection, identification, and measurement of concentration of a wide variety of biologically important molecules. These analytical techniques can be classified into three general categories: biological techniques or “bioassays,” those based on physical and/or chemical methods, and those that depend on noncovalent binding of one reactant to another. Techniques that fall under the third category are also commonly referred to as “binding assays.” The salient features of these classes of analytical techniques are briefly described below.


Yersinia Enterocolitica Product Development Process Maleic Hydrazide Thyroxine Binding Globulin Immunodiagnostic Test 
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  1. Avrameas, S., and Uriel, J. 1966. Methode de marquage d’antigenes et d’anticorps avec des enzymes et son application en immunodiffusion. C. R. Acad. Sci. Paris 262:2543-2545.Google Scholar
  2. Bangham, D. R. 1983. Reference materials and standardization. In Principles of Competitive Protein-Binding Assays, eds. W. D. Odell and P. Franchimont, pp. 85–105, John Wiley, New York.Google Scholar
  3. Barnard, G.; Kohen, F.; Mikola, H.; and Lovgren, T. 1989. Measurement of estrone-3-glucuronide in urine by rapid, homogeneous time-resolved fluoroimmunoassay. Clin. Chem. 35:555–559.PubMedGoogle Scholar
  4. Butler, J. E. 1991. Perspectives, configurations and principles. In Immunochemistry of Solid-Phase Immunoassay, ed. J. E. Butler, pp. 3–26, CRC Press, Boca Raton, FL.Google Scholar
  5. Campbell, P. J. 1974. International biological standards and reference preparations. J. Biol. Standardization 2:249–258.CrossRefGoogle Scholar
  6. Catt, K., and Tregear, G. W. 1967. Solid phase radioimmunoassay in antibody-coated tubes. Science 158:1570–1572.PubMedCrossRefGoogle Scholar
  7. Catty, D. 1988. Antibodies. A Practical Approach. IRL Press, Oxford.Google Scholar
  8. Clausen, J. 1988. Immunochemical Techniques for the Identification and Estimation of Macromolecules. Elsevier, Amsterdam.Google Scholar
  9. Coombs, R. R. A.; Mourant, A. E.; and Race, R. R. 1945. Certain properties of antiserums prepared against human serum and its various protein fractions. Their use in the detection of sensitization of human red cells with incomplete Rh antibody and on the nature of this antibody. Br. J. Exp. Pathol. 26:255–263.PubMedGoogle Scholar
  10. Coons, A. H.; Creech, H. J.; and Jones, R. H. 1941. Immunological properties of an antibody containing a fluorescent group. Proc. Soc. Exp. Biol. Med. 47:200–202.Google Scholar
  11. Deshpande, S. S., and Sharma, B. P. 1993. Immunoassays, nucleic acid probes, and biosensors. Two decades of development, current status and future projections in clinical, environmental and agricultural applications. In Diagnostics in the Year 2000, eds. P. Singh, B. P. Sharma, and P. Tyle, pp. 459–525, Van Nostrand Reinhold, New York.Google Scholar
  12. Diamandis, E. P. 1988. Immunoassays with time-resolved fluorescence spectroscopy: principles and applications. Clin. Biochem. 21:139–150.PubMedGoogle Scholar
  13. Diamandis, E. P. 1990. Detection techniques for immunoassay and DNA probing applications. Clin. Biochem. 23:437-443.PubMedCrossRefGoogle Scholar
  14. Diamandis, E. P. 1991. Multiple labeling and time-resolvable fluorophores. Clin. Chem. 37:1486–1491.PubMedGoogle Scholar
  15. Ekins, R. P. 1960. The estimation of thyroxine in human plasma by an electrophoretic technique. Clin. Chim. Acta 5:453–459.PubMedCrossRefGoogle Scholar
  16. Ekins, R. P. 1989. Multi-analyte immunoassay. J. Pharm. Biomed. Anal. 7:155–168.PubMedCrossRefGoogle Scholar
  17. Elek, S. D. 1948. Recognition of toxicogenic bacterial strains in vitro. Br. Med. J. 1:493-496.PubMedCrossRefGoogle Scholar
  18. Engvall, E., and Perlmann, P. 1971. Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry 8:871–874.PubMedCrossRefGoogle Scholar
  19. Farr, R. S. 1958. A quantitative immunochemical measure of the primary interaction between I*BSA and antibody. J. Infect. Dis. 103:239–262.PubMedCrossRefGoogle Scholar
  20. Gibbons, I. 1985. Nonseparation enzyme immunoassays for macromolecules. In Enzyme-Mediated Immunoassay, ed. T. T. Ngo and H. M. Lenhoff, pp. 121–144, Plenum Press, New York.Google Scholar
  21. Gosling, J. P. 1990. A decade of development in immunoassay methodology. Clin. Chem. 36:1408–1427.PubMedGoogle Scholar
  22. Graber, P., and Williams, C. A. 1953. Method permitting the simultaneous study of electrophoretic and immunochemical properties of a mixture of proteins. Application to blood serum. Biochim. Biophys. Acta 10:193–194.CrossRefGoogle Scholar
  23. Hawcroft, D.; Hector, T.; and Rowell, F. 1987. Quantitative Bioassay. John Wiley, New York.Google Scholar
  24. Heidelberger, M. 1939. Chemical aspects of the precipitin and agglutinin reactions. Chem. Rev. 24:323–343.CrossRefGoogle Scholar
  25. Hemmila, I. 1985. Fluoroimmunoassays and immunofluorometric assays. Clin. Chem. 31:359–370.PubMedGoogle Scholar
  26. Humphrey, J. H., and White, R. G. 1964. Immunology for Students of Medicine. Black-well Scientific, Oxford.Google Scholar
  27. Kirkwood, T. B. L. 1977. Predicting the stability of biological standards and products. Biometrics 33:736–742.PubMedCrossRefGoogle Scholar
  28. Landsteiner, K. 1945. The Specificity of Serologic Reactions. Harvard University Press, Cambridge, MA.Google Scholar
  29. Laurell, C. B. 1966. Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. Anal. Biochem. 15:45–52.PubMedCrossRefGoogle Scholar
  30. Litman, D. J. 1985. Test strip enzyme immunoassay. In Enzyme-Mediated Immunoassay, ed. T. T. Ngo and H. M. Lenhoff, pp. 155–190, Plenum Press, New York.Google Scholar
  31. Mancini, G.; Carbonara, A. O.; and Heremans, J. F. 1965. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry 2:235–254.PubMedCrossRefGoogle Scholar
  32. Nakane, P. K., and Pierce, G. B. 1966. Enzyme-labeled antibodies preparation and application for the localization of antigens. J. Histochem. Cytochem. 14:929–931.PubMedCrossRefGoogle Scholar
  33. Ngo, T. T., and Lenhoff, H. M. 1985. Enzyme-Mediated Immunoassay. Plenum Press, New York.Google Scholar
  34. Odell, W. D. 1983. Principles of in vitro bioassays. In Principles of Competitive Protein-Binding Assays, eds. W. D. Odell and P. Franchimont, pp. 267–279, John Wiley, New York.Google Scholar
  35. Odell, W. D., and Franchimont, P. 1983. Principles of Competitive Protein-Binding Assays. John Wiley, New York.Google Scholar
  36. Ouchterlony, O. 1948. Antigen-antibody reactions in gels. Acta Path. Microbiol. 26: 507–515.CrossRefGoogle Scholar
  37. Oudin, J. 1946. Method of immunochemical analysis by specific precipitation in gelled medium. C. R. Acad. Sci. Paris 222:115–116.Google Scholar
  38. Pettersson, K. 1993. Comparison of immunoassay technologies. Clin. Chem. 39: 1359–1360.PubMedGoogle Scholar
  39. Rubenstein, K. E.; Schneider, R. S.; and Ullman, E. F. 1972. “Homogeneous” enzyme immunoassay. A new immunochemical technique. Biochem. Biophys. Res. Commun. 47:846–851.PubMedCrossRefGoogle Scholar
  40. Singer, S. J., and Schick, A. 1961. On the formation of covalent linkages between two protein molecules. J. Biol. Chem. 236:2477–2481.PubMedGoogle Scholar
  41. Soini, E., and Hemmila, I. 1979. Fluoroimmunoassay: present status and key problems. Clin. Chem. 25:353–361.PubMedGoogle Scholar
  42. Steward, M. W. 1984. Antibodies. Their Structure and Function. Chapman & Hall, New York.Google Scholar
  43. Tiselius, A., and Kabat, E. 1939. An electrophoretic study of immune serums and purified antibody preparations. J. Exp. Med. 69:119–131.PubMedCrossRefGoogle Scholar
  44. van Weeman, B. K., and Schuurs, A. H. W. N. 1971. Immunoassay using antigen-enzyme conjugates. FEBS Lett. 15:232–236.CrossRefGoogle Scholar
  45. Wild, D. 1994. The Immunoassay Handbook. Stockton Press, New York.Google Scholar
  46. Yalow, R. S., and Berson, S.A. 1959. Assay of plasma insulin in human subjects by immunological methods. Nature (London) 184:1648–1649.CrossRefGoogle Scholar
  47. Yalow, R. S., and Berson, S. A. 1960. Immunoassay of endogenous plasma insulin in man. J. Clin. Invest. 39:1157–1175.PubMedCrossRefGoogle Scholar

Copyright information

© Chapman & Hall 1996

Authors and Affiliations

  • S. S. Deshpande
    • 1
  1. 1.Idetek, Inc.USA

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