The human gallbladder

  • Liliana Luciano
  • Enrico Reale
Part of the Electron Microscopy in Biology and Medicine book series (EMBM, volume 6)

Abstract

The human gallbladder (vesica fellea) is a pear-shaped sac inserted between the under surface of the right lobe of the liver and the peritoneal serous membrane. It consists of three parts or regions: the neck, body (representing the main part of the organ), and fundus (a blind end). The neck is in continuity with the cystic duct, which connects the gallbladder with the common bile duct (ductus choledochus). The lack of morphological information concerning the normal human gallbladder, especially at the level of electron microscopy, was mentioned by Wallraff [1] and remains virtually unchanged to the present day. The reason for this lies in the difficulty or even impossibility of 1) obtaining healthy organs and 2) processing them quickly enough in order to achieve a satisfactory morphology. Most of our present knowledge on the structure and function of the gallbladder stems from studies on animals.

Keywords

Mast Cell Lamina Propria Dense Body Gallbladder Wall Principal Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Wallraff J: Gallengangsystem, Gallenblase und Galle. In: Handbuch der Mikroskopischen Anatomie, Vol V/4. W Bargmann, (ed), Berlin: Springer, 277–363, 1969.Google Scholar
  2. 2.
    Schmidt CR, Ivy AC: The general function of the gall bladder. J Cell Comp Physiol 10: 365–383, 1937.CrossRefGoogle Scholar
  3. 3.
    Edholm P: Gallbladder evacuation in the normal male induced by cholecystokinin. Acta Radiol 53: 257–265, 1960.PubMedCrossRefGoogle Scholar
  4. 4.
    Mackie CR, Baxter JN, Grime JS, Hulks G, Cuschieri A: Gall bladder emptying in normal subjects — a data base for clinical cholescintigraphy. Gut 28: 137–141, 1987.PubMedCrossRefGoogle Scholar
  5. 5.
    Diamond JM: Transport mechanisms in the gallbladder. In: Handbook of Physiology: Alimentary Canal. CF Code (ed), Washington DC: American Physiological Society, 2451–2482 1969.Google Scholar
  6. 6.
    Geenen JE, Hogan WJ, Dodds WJ, Stewart ET, Arndorfer RC: Intraluminal pressure recording from the human sphincter of Oddi. Gastroenterology 78: 317–324, 1980.PubMedGoogle Scholar
  7. 7.
    Torsoli A, Ramorino ML, Alessandrini A: Motility of the biliary tract. Rendic R Gastroenterol 2: 67–80, 1970.Google Scholar
  8. 8.
    Whitehouse RW, Martin DF: Contrast-enhanced computed tomography of the normal and abnormal gallbladder. Br J Radiol 59: 1083–1085, 1986.PubMedCrossRefGoogle Scholar
  9. 9.
    Engel JM, Deitch EA, Sikkema W: Gallbladder wall thickness: sonographic accuracy and relation to disease. Am J Roentgenol 134: 907–909, 1980.Google Scholar
  10. 10.
    Fawcett DW: A Textbook of Histology, 11th ed. Philadelphia: WB Saunders, 1986.Google Scholar
  11. 11.
    Luciano L, Reale E, Wolpers C: Die Feinstruktur der Gallenblase und der Gallengänge. V. Histochemische Lokalisierung von Mukosustanzen im menschlichen Gallenblasenepithel. Histochemistry 38: 57–70, 1974.CrossRefGoogle Scholar
  12. 12.
    Thiéry JP: Mise en évidence des polysaccharides sur coupes fines en microscopie électronique. J Microscopie 6: 987–1018, 1967.Google Scholar
  13. 13.
    Mowry RW: Improved procedure for the staining of acidic polysaccharides by Müller’s colloidal (hydrous) ferric oxide and its combination with the Feulgen and the periodic acid-Schiff reactions. Lab Invest 7: 566–576, 1958.PubMedGoogle Scholar
  14. 14.
    Yoshitomi S, Miyazaki K, Nakayama F: Demonstration and maintenance of mucus secretion in cultured human gallbladder epithelial cells. In Vitro Cell Develop Biol 23: 559–566, 1987.CrossRefGoogle Scholar
  15. 15.
    Luciano L, Wermbter G, Reale E: Die Feinstruktur der Gallenblase und der Gallengänge. III. Beobachtungen an gefriergeätzten Präparaten der Gallenblase der Maus unter besonderer Berücksichtigung der dichten Körper und des Verbindungskomplexes. Cytobiologie 7: 76–88, 1973.Google Scholar
  16. 16.
    Nagahiro K: Zytologische Untersuchungen über die Epithelzelle der Gallenblase des Menschen. Cytologia (Tokyo) 9: 132–163, 1938.Google Scholar
  17. 17.
    Luciano L: Die Feinstruktur der Gallenblase und der Gallengänge. I. Das Epithel der Gallenblase der Maus. Z Zellforsch 135: 87–102, 1972.PubMedCrossRefGoogle Scholar
  18. 18.
    Yamada E: The fine structure of the gall bladder epithelium of the mouse. J Biophys Biochem Cytol 1: 445–458, 1955.PubMedCrossRefGoogle Scholar
  19. 19.
    Hayat MA: Fixation for Electron Microscopy. New York: Academic Press, 1981.Google Scholar
  20. 20.
    Pfuhl W: Die Gallenblase und die extrahepatischen Gallengänge. In: Handbuch der Mikroskopishen Anatomie des Menschen, vol 5. von W Möllendorff (ed), Berlin: Springer Verlag, 426–462, 1932.Google Scholar
  21. 21.
    Evett RD, Higgins JA, Brown AL Jr: The fine structure of normal mucosa in human gall bladder. Gastroenterology 47: 49–60, 1964.PubMedGoogle Scholar
  22. 22.
    Bader G: Die submikroskopische Struktur des Gallenbla-senepithels. III. Mitteilung. Das Epithel der Steingallenblase des Menschen. Frank Z Path 74: 501–511, 1965.Google Scholar
  23. 23.
    Bartels H, Welsch U: Freeze fracture study of the turtle lung. 2. Rod-shaped particles in the plasma membrane of a mitochondria-rich pneumocyte in Pseudemys (Chrysemys) scripta. Cell Tissue Res 236:453–457, 1984.PubMedCrossRefGoogle Scholar
  24. 24.
    Wallraff J, Dietrich KF: Zur Morphologie and Histochemie der Steingallenblase des Menschen. Z Zellforsch 46: 155–231, 1957.PubMedCrossRefGoogle Scholar
  25. 25.
    Toledo OMS, Morales CR, Pereyra LA, Jordao T, Montes GS: Migrating mast cells in the gallbladder epithelium of cattle and sheep. Histochemistry 72: 433–442, 1981.PubMedCrossRefGoogle Scholar
  26. 26.
    Enerback L: Mast cell heterogeneity: the evolution of the concept of a specific mucosal mast cell. In: Mast Cell Differentiation and Heterogeneity. AD Befus, J Bienenstock, JA Denburg (eds). New York: Raven Press, 1–26, 1986.Google Scholar
  27. 27.
    Solcia E, Capelra C, Vassallo G, Buffa R: Endocrine cells of the gastric mucosa. Int Rev Cytol 42: 223–286, 1975.PubMedCrossRefGoogle Scholar
  28. 28.
    Luciano L, Reale E: A new morphological aspect of the brush cells of the mouse gallbladder epithelium. Cell Tissue Res 201: 37–44, 1979.PubMedCrossRefGoogle Scholar
  29. 29.
    Luciano L, Castellucci M, Reale E: The brush cells of the common bile duct of the rat. Thin section, freeze-fracture and scanning electron microscopy. Cell Tissue Res 218: 403–420, 1981.PubMedCrossRefGoogle Scholar
  30. 30.
    Erspamer V: Die enterochromaffinen Zellen der Gallenwege in normalen und pathologischen Zuständen. (Nach Untersuchungen beim Menschen und bei Säugetieren.) Virchows Arch 296: 70–92, 1936.Google Scholar
  31. 31.
    Erspamer V: Sulla presenza di cellule argentofile (preenterocromaffini) nelle vie biliari dell’uomo e di alcuni mammiferi. Anat Anz 85: 272–284, 1938.Google Scholar
  32. 32.
    Laitio M, Nevalainen T: Ultrastructure of endocrine cells in metaplastic epithelium of human gall bladder. J Anat 120: 219–225, 1975.PubMedGoogle Scholar
  33. 33.
    Claude P, Goodenough DA: Fracture faces of zonulae occludentes from “tight” and “leaky” epithelia. J Cell Biol 58: 390–400, 1973.PubMedCrossRefGoogle Scholar
  34. 34.
    Bullivant S: Possible relationships between tight junction structure and function. In: Epithelial Ion and Water Transport. ADC Micknight, JP Leader (eds), New York: Raven Press, 265–275, 1981.Google Scholar
  35. 35.
    Castellucci M, Familiari G, Caggiati A: Heterogeneous distribution of ruthenium red-proteoglycan aggregates in the basement membrane (BM) of the gallbladder epithelium. In: The Extracellular Matrix: Chemistry, Biology, Pathology. J Jeffry, B Mecham (eds), St Louis: Washington University Press, 1982.Google Scholar
  36. 36.
    Montes GS, Bezerra MSF, Junqueira LCU: Collagen distribution in tissues. In: Ultrastructure of the Connective Tissue Matrix. A Ruggeri, PM Motta (eds), Boston: Martinus Nijhoff Publishers, 65–88, 1984.Google Scholar
  37. 37.
    Ivy AC, Oldberg E: A hormone mechanism for gallbladder contraction and evacuation. Am J Physiol 86: 599–613, 1928.Google Scholar
  38. 38.
    Mutt V, Jorpes JE: Structure of porcine cholecystokinin-pancreozyimin. 1. Cleavage with thrombin and with trypsin. Eur J Biochem 6: 156–162, 1968.PubMedCrossRefGoogle Scholar
  39. 39.
    Morley JE: The ascent of cholecystokinin (CCK) — from Gut to brain. Life Sci 30: 479–493, 1982.PubMedCrossRefGoogle Scholar
  40. 40.
    Walsh JH: Gastrointestinal Hormones. In: Physiology of the Gastrointestinal Tract, 2nd ed. LR Johnson (ed), New York: Raven Press, 181–253, 1987.Google Scholar
  41. 41.
    Ryan JP: Motility of the gallbladder and biliary tree. In: Physiology of the Gastrointestinal Tract, 2nd ed. LR Johnson (ed), New York: Raven Press, 695–721, 1987.Google Scholar
  42. 42.
    Byrnes DJ, Borody T, Daskalopoulos G, Boyle M, Benn I: Cholecystokinin and gallbladder contraction: effect of CCK infusion. Peptides 2: 259–262, 1981.PubMedCrossRefGoogle Scholar
  43. 43.
    Wiener I, Inoue K, Fagan CJ, Lilja P, Watson LC, Thompson JC: Release of cholecystokinin in man. Correlation of blood levels with gallbladder contraction. Ann Surg 194: 321–325, 1981.PubMedCrossRefGoogle Scholar
  44. 44.
    Lilja P, Fagan CJ, Wiener I, Inoue K, Watson LC, Rayford PL: Infusion of pure cholecystokinin in humans. Correlation between plasma concentrations of cholecystokinin and gallbladder size. Gastroenterology 83: 256–261, 1982.PubMedGoogle Scholar
  45. 45.
    Sacchetti G, Mandelli V, Roncoroni L, Montanari C: Influence of age and sex on gallbladder emptying induced by a fatty meal in normal subjects. Am J Roentgenol 119: 40–50, 1973.Google Scholar
  46. 46.
    Kaene P, Colwell D, Baer HP, Clanachan AS, Scott GW: Effects of age, gender and female sex hormones upon contractility of the human gallbladder in vitro. Surg Gynecol Obstet 163: 555–560, 1986.Google Scholar
  47. 47.
    Upp JR, Nealon WH, Singh P, Fagan CJ, Jonas AS, Greeley GH, Thompson JC: Correlation of cholecy-stokinin receptors with gallbladder contractility in patients with gallstones. Ann Surg 205: 641–648, 1987.PubMedCrossRefGoogle Scholar
  48. 48.
    LaMorte WW, Schoetz DJ, Birkett DH, Williams LF: The role of the gallbladder in the pathogenesis of cholesterol gallstones. Gastroenterology 77: 580–592, 1979.PubMedGoogle Scholar
  49. 49.
    Chevreul M: Recherches chimiques sur les corps gras, et particulièrement sur leurs combinaisons avec les alcalis. Sixième Mémoire. Examen des graisses d’homme, de mouton, de boeuf, de jaguar et d’oie. Ann Chim Phys 2: 339–372, 1816.Google Scholar
  50. 50.
    Berthelot M: Sur plusieurs alcools nouveaux. Combinaisons des acides avec la Cholestérine, l’éthal, le camphre de Bornéo et la méconine. Ann Chim Phys 56: 51–98, 1859.Google Scholar
  51. 51.
    Mead JF, Alfin-Slater RB, Howton DR, Popjak G: Biosynthesis of cholesterol and related substances. In: Lipids. Chemistry, Biochemistry, and Nutrition. New York: Plenum Press, 295–367, 1986.Google Scholar
  52. 52.
    Cooperberg PL, Gibney RG: Imaging of the gallbladder, 1987. Radiology 163: 605–613, 1987.PubMedGoogle Scholar
  53. 53.
    Glenn F: Biliary tract disease since antiquity. Bull NY Acad Med 47: 329–350, 1971.Google Scholar
  54. 54.
    Gracie WA, Ransohoff DF: The natural history of silent gallstones. The innocent gallstone is not a myth. N Engl J Med 307: 798–800, 1982.PubMedCrossRefGoogle Scholar
  55. 55.
    Wolpers C, Blaschke R: Electron microscopy of human gallstones. In: 29th Ann Proc Electron Microscopy Soc Amer. CJ Arceneaux (ed), Baton Rouge, LA: Claitor’s Publishing Division, 296–297, 1971.Google Scholar
  56. 56.
    Wolpers C: Gallenblasensteine. Ihre Morphogenese und Auswahl zur Litholyse. Basel: Karger Verlag, 1987.Google Scholar
  57. 57.
    Myant NB: Cholesterol gallstones; plasma cholesterol in liver disease. In: The Biology of Cholesterol and Related Steroids. London: Heinemann Medical Books, 853–387, 1981.Google Scholar
  58. 58.
    L Capocaccia, G Ricci, F Angelico, M Angelico, AF Attili (eds): Epidemiology and Prevention of Gallstone Disease. Lancaster: MTP Press, 1984.Google Scholar
  59. 59.
    Holzbach RT: Recent progress in understanding cholesterol crystal nucleation as a precursor to human gallstone formation. Hepatology 6: 1403–1406, 1986.PubMedCrossRefGoogle Scholar
  60. 60.
    Smith BF: Human gallbladder mucin binds biliary lipids and promotes cholesterol crystal nucleation in model bile. J Lipid Res 28: 1088–1097, 1987.PubMedGoogle Scholar
  61. 61.
    Nevalainen T, Laitio M: Ultrastructure of gallbladder with cholesterolosis. Virchows Arch Abt B Zellpath 10: 237–242, 1972.Google Scholar
  62. 62.
    Luciano L, Wolpers C: Die Feinstruktur der Gallenblase und der Gallengänge. IV. Die Cholesterose der menschlichen Gallenblasenschleimhaut. Virchows Arch Abt B Zellpath 14: 147–158, 1973.Google Scholar
  63. 63.
    Koga A: Fine structure of the human gallbladder with cholesterosis with special reference to the mechanism of lipid accumulation. Br J Exp Path 66: 605–611, 1985.Google Scholar
  64. 64.
    Williamson JR: Ultrastructural localization and distribution of free cholesterol (3β hydroxysterols) in tissues. J Ultrastruct Res 27: 118–133, 1969.CrossRefGoogle Scholar
  65. 65.
    Koga A, Todo S, Nishimura M: Electron microscopic observations on the cholesterol distributed in the epithelial cells of the gallbladder. Histochemistry 44: 303–306, 1975.PubMedCrossRefGoogle Scholar
  66. 66.
    Elias PM; Goerke J, Friend DS: Freeze-fracture identification of sterol-digitonin complexes in cell and liposome membranes..J Cell Biol 78: 577–596, 1978.PubMedCrossRefGoogle Scholar
  67. 67.
    Rose RC: Absorptive functions of the gallbladder. In: Physiology of the Gastrointestinal Tract, 2nd ed. LR Johnston (ed), New York: Raven Press, 1455–1468, 1987.Google Scholar
  68. 68.
    Kaye GI, Wheeler HO, Whitlock RT, Lane N: Fluid transport in the rabbit gallbladder. A combined physiological and electron microscopic study. J Cell Biol 30: 237–268, 1966.PubMedCrossRefGoogle Scholar
  69. 69.
    Castellucci M, Caggiati A: Surface aspects of the rabbit gallbladder mucosa and their functional implications. J Submicrosc Cytol 12: 375–390, 1980.Google Scholar
  70. 70.
    Nielsen OV, Nielsen ML, Lauritzen KEF: Examination of the mucosa of the normal human gall bladder by scanning electron microscopy. Micron 5: 281–291, 1975.Google Scholar

Copyright information

© Kluwer Academic Publishers 1990

Authors and Affiliations

  • Liliana Luciano
    • 1
  • Enrico Reale
  1. 1.Medizinische Hochschule HannoverAbteilung Zellbiologie und ElektronenmikroskopieHannover 61Federal Republic of Germany

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