Effects of a DNA Intercalator, Ethidium Bromide on Chromatin Structure of Chicken Erythrocyte

  • Zhu Jing-de
  • Sun Xiao-ping
  • Wang Fan
Chapter

Abstract

DNase I hypersensitive sites (DHS) are the most loosely packed regions in chromatin, which are ultrasensitive, typically 100 times more than bulk chromatin, to DNase I as well as other DNA cleaving agents including methidiumpropy EDTA, a DNA intercalator (Elgin, 1988; Gruss and Garrard, 1988). DHS have been mapped to many functionally crucial regions, including promoter, enhancer, silencer and terminator of transcription, DNA replication origins, recombination elements as well as the centromere and telomere. Relevant to the transcription of genes, the occurrence of DHS can be constitutive or inducible, and developmentally or tissue-specifically regulated. DHS have been correllated with the chromatin region of nucleosome-free, or of atypical DNA structure, or associated with trans-acting protein or proteins, or both. Conformational polymorphism of the DNA component of genome, notably bending, unpairing, cruciform and Z-form, is both sequence and environment-dependant (Rich et al., 1984; McLean and Wells, 1988; Jaworski et al., 1987). DNA supercoiling (Tsao et al., 1989) and DNA binding proteins are two important environmental factors. The former may also play a role in formation and maintenance of DHS (Villeponteau, et al., 1984). DNA-protein interaction, especially the sequence-specific interaction (Pabo and Sauer, 1986; Dynan and Tjian, 1985), may also participate in formation of DHS in vivo (Emerson and Felsenfeld, 1984). DNA intercalators alter DNA conformation by inserting their planer aromatic rings between the base pairs of the neighbouring nucleotides, without impairing the structural integrity of DNA (Neidle and Abraham, 1984).

Keywords

Ethidium Bromide Globin Gene Chicken Erythrocyte Globin Gene Cluster Planer Aromatic Ring 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References

  1. Dynan W. S., and Tjian R., 1985, Control of eukaryotic messenger RNA synthesis by sequence—specific DNA binding proteins, Nature, 316:774.CrossRefGoogle Scholar
  2. Elgin S. C. R., 1988, The formation and function of DNase I hypersensitive sites in the process of gene activation, J. Biol. Chem., 263(36):19259.Google Scholar
  3. Felsenfeld G., Emerson B. M., Jackson P. D., Hesse J. E., Liever M. R., and Nickol J. M., 1986, Chromatin structure near an active gene, In ‘New frontier in the study of gene function’, Poste G., and Cook S. T., eds, Plenum, New York.Google Scholar
  4. Gruss D. S., and Garrard W. T., 1988, Nuclease hypersensitive sites in chromatin, Ann. Rev. Biochem., 57:159.CrossRefGoogle Scholar
  5. Jaworski A., Hsieh W-T., Balho J. A., Larson J. E., and Wells R. D., 1987, Left—handed DNA in vivo, Science, 238:773.CrossRefGoogle Scholar
  6. McLean M., and Wells R. D., 1988, The role of sequences in the stabilization of left—handed DNA helices in vitro, BBA, 950:243.Google Scholar
  7. McMurry C. T., and Van Holde K. E., 1986, Binding of ethidium bromide causes dissociation of nucleosome core particle, Proc. Natl. Acad. Sci. USA, 83:8472.CrossRefGoogle Scholar
  8. Neidle S., and Abraham Z., 1986, Structural and sequence—dependent aspects of drug interaction into nucleic acids, CRC Critical Reviews in Biochemistry, 17(1):73.CrossRefGoogle Scholar
  9. Pabo C, and Sauer R. T., 1986, Protein—DNA recognition, Ann. Rev. Biochem., 55:292.Google Scholar
  10. Rich A., Nordeheim A., and Wang A. H. J., 1984, The chemistry and biology of left—handed Z-DNA, Ann. Rev. Biochem., 53:791.CrossRefGoogle Scholar
  11. Schon E., Evans T., Welsh J., and Efstratiadis A., 1983, Conformation of promoter DNA: fine mapping of S 1-hypersensitive sites, Cell, 35:837.CrossRefGoogle Scholar
  12. Schoter H., Maier G., Ponsting H., and Nordheim A., 1985, DNA intercalator induce specific release of HMG14, HMG17 and other DNA—binding proteins from chicken erythrocyte chromatin, The EMBQ J., 4(13B):3867.Google Scholar
  13. Tsao Y-P., Wu H-Y, Liu L. F., 1989, Transcription—driven supercoiling of DNA: direct biochemical evidence form in vitro studies, Cell, 56:111.CrossRefGoogle Scholar
  14. Villeponteau B., Lundell M., Martison H., 1984, Torsional stress promotes the DNase I sensitivity of active genes, Cell, 39:469.CrossRefGoogle Scholar
  15. Wang F., and Zhu J. D., 1989, The effects of DNA intercalators on chromatin of the chicken red blood cells— Differential extraction of nonhistone proteins, Cell Research, 1:105.Google Scholar

Copyright information

© Plenum Press, New York 1990

Authors and Affiliations

  • Zhu Jing-de
    • 1
  • Sun Xiao-ping
    • 1
  • Wang Fan
    • 1
  1. 1.Shanghai Institute of Cell Biology Shanghai Open Laboratory in Life SciencesAcademia SinicaShanghaiPR China

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