Rapid Diagnosis of Viral Infections

  • Thomas F. Smith
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 263)


Rapid tests for use in the virology laboratory have evolved because of the recognition of serious complications produced by viruses and the availability of antiviral therapy such as acyclovir and gancyclovir to influence the clinical course of the patient. Accordingly, many of these systemic viral infections occur as a result of impaired host immune defense mechanisms. Thus, the importance of the herpesviruses in particular, producing life-threatening disease in organ transplant recipients (kidney, bone marrow, liver, heart, lung, and pancreas) has been recognized for several years. The severe immunosuppression induced in patients infected with human immunodeficiency virus (HIV) has amplified the importance of underlying infection with herpesviruses, and hence laboratory diagnosis of these infections in this population.


Respiratory Syncytial Virus Herpes Simplex Virus Type Genital Herpes Early Antigen Shell Vial 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Adler-Storthz, K., Kendall, C., Kennedy, R. C., Henkel, R. D., and Dreesman, G. R., 1983, Biotin-avidin-amplified enzyme immunoassay for detection of herpes simplex virus antigen in clinical specimens, J Clin Microbiol. 18:1329.PubMedGoogle Scholar
  2. Almeida, J. D., 1980, Practical aspects of diagnostic electron microscopy, Yale J Biol Med., 53:5.PubMedGoogle Scholar
  3. Baker, D. A., Pavan-Langston, D., Gonick, B., Milch, P. O., Dunkel, E. C., Berkowitz, A., Vander-Mallie, R., Woodin, M. B., Philip, A., Bobrow, M. N., and Cukor, G, 1987, Multicenter clinical evaluation of DuPont’s Herpchek“, a new rapid diagnostic test for herpes simplex virus (HSV), Abstract, Eastern Pennsylvania Branch, ASM, Philadelphia, PA.Google Scholar
  4. Brautigan, A. R., Richman D. D., and Oxman, M. N., 1980, Rapid typing of herpes simplex virus isolates by deoxyribonucleic acid: deoxyribonucleic acid by hybridization, J Clin Microbiol. 12:226.Google Scholar
  5. Chernesky, M. A., Ray, C. G., Smith, T. F., and Drew, W. L., 1982, Laboratory diagnosis of viral infections, Cumitech 15:1.Google Scholar
  6. Gleaves, C. A., Smith, T. F., Shuster, E. A., and Pearson, G. R., 1984, Rapid detection of cytomegalovirus in MRC-5 cells inoculated with urine specimens by using low-speed centrifugation and monoclonal antibody to an early antigen, J Clin Microbiol. 19:917.PubMedGoogle Scholar
  7. Hallsworth, P. G., and McDonald, P. J., 1985, Rapid diagnosis of viral infections with fluorescent antisera, Pathology 17:629.PubMedCrossRefGoogle Scholar
  8. Josephson, S. L., 1985, Immunoenzyme techniques, Clin Microbiol Newsletter 7:81.CrossRefGoogle Scholar
  9. Macasaet, F. F., Holley, K. E., Smith, T. F., and Keys, T. F., 1975, Cytomegalovirus studies of autopsy tissue. H. Incidence of inclusion bodies and related pathologic data, Am J Clin Pathol. 63:859.PubMedGoogle Scholar
  10. Michalski, F. J., Shaikh, M., Sahraie, F., Desai, S., Verano, L., and Vallabhanine, J., 1986, Enzyme-linked immunosorbent assay spin amplification technique for herpes simplex virus antigen detection, J Clin Microbiol. 24:310.PubMedGoogle Scholar
  11. Miller, S. E., and Lang, D. J., 1982, Rapid diagnosis of herpes simpex infection: amplification for electron-microscopy by short-term in vitro replication, J Infect. 4:37.PubMedCrossRefGoogle Scholar
  12. Montplaisir, S., Belloncik, S., Leduc, N. P., Angi, P. A., Martineau, B., and Kurstak, E., 1972, Electron microscopy in the rapid diagnosis of cytomegalovirus: ultrastructural observation and comparison of methods of diagnosis, J Infect Dis., 125:533.PubMedCrossRefGoogle Scholar
  13. Morgan, M. A., and Smith, T. F., 1894, Evaluation of an enzyme-linked immunosorbent assay for the detection of herpes simplex virus antigen, J Clin Microbiol. 19:730.Google Scholar
  14. Moseley, R. C., Corey, L., Benjamin, D., Winter, C., and Remington, M. J., 1981, Comparison of viral isolation, direct immunofluorescence and indirect immunoperoxidase techniques for detection of genital herpes simplex virus infection, J Clin Microbiol. 13:913.PubMedGoogle Scholar
  15. Nakane, P. K., and Pierce, G. B., 1966, Enzyme-linked antibodies: preparation and application for localization of antigens, J Histochem Cytochem. 14:929.PubMedCrossRefGoogle Scholar
  16. Nerurkar, L. S., Namba, M., Brashears, G., Jacob, A. J., Lee, Y. J., and Sever;. J. L., 1984, Rapid detection of herpes simplex virus in clinical specimens by use of a capture biotin-streptavidin enzyme-linked immunosorbent assay, J Clin Microbiol. 28:109.Google Scholar
  17. Paya, C. V., Wold, A. D., and Smith, T. F., 1987, Detection of cytomegalovirus infections in specimens other than urine by the shell vialassay and conventional tube cell cultures, J Clin Microbiol. 25:755.PubMedGoogle Scholar
  18. Richman, D. D., Cleveland, P. H., Redfield, D. C., Oxman, M. N., and Wahl, G. M., 1984, Rapid viral diagnosis, J Infect Dis., 149:298.PubMedCrossRefGoogle Scholar
  19. Shuster, E. A., Beneke, J. S., Tegtmeier, G. E., Pearson, G. R., Gleaves, C. A., Wold, A. D., and Smith, T. F., 1985, Monoclonal antibody for rapid laboratory detection of cytomegalovirus infections: characterization and diagnostic application, Mayo Clin Proc. 60:577.PubMedGoogle Scholar
  20. Smith, T. F., 1987, Rapid methods for the diagnosis of viral infections, Lab Med., 18:16.Google Scholar
  21. Thiele, G. M., Bicak, M. S., Young, A., Kinsey, J., White, R. J., and Purtilo, D. T., 1987, Rapid detection of cytomegalovirus by tissue culture, centrifugation, and immunofluorescence with a monoclonal antibody to an early nuclear antigen, J Virol Meth., 16:327.CrossRefGoogle Scholar
  22. van Ulsen, J., Dumas, A. M., Vagenvoort, J. H. T., van Zuuren, A., van Joost, T., and Stolz, E., 1987, Evaluation of an enzyme immunoassay for detection of herpes simplex virus antigen in genital lesions, Eur J Clin Microbiol. 6:410.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Thomas F. Smith
    • 1
  1. 1.Section of Clinical MicrobiologyMayo Clinic and Mayo FoundationRochesterUSA

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