In Vitro Aspects of Erythropoiesis pp 31-36 | Cite as
Culture Systems In Vitro for the Assay of Erythrocytic and Megakaryocytic Progenitors
Chapter
Abstract
The production of erythrocytic colonies from murine fetal liver cells seeded in semisolid plasma culture medium was first accomplished in 1971.1 The plasma culture system was then improved2 and is now widely used along with the methylcellulose system3 for the assay of the erythropoietic progenitors, CFU-e and BFU-e, of adult as well as fetal hemopoietic tissues of several species including man.
Keywords
Spleen Cell Plasma Clot Bovine Serum Albumin Solution Large Petri Dish Agar Clot
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References
- 1.Stephenson JR, Axelrad AA, McLeod DL, et al: Induction of colonies of hemoglobin-synthesizing cells by erythropoietin in vitro. Proc Natl Acad Sci USA. 68: 1542–1546, 1971CrossRefGoogle Scholar
- 2.McLeod DL, Shreeve MM, Axelrad AA: Improved plasma culturesystem for production of erythrocytic colonies in vitro: Quantitative assay method for CFU-e. Blood 44: 517–534, 1974.PubMedGoogle Scholar
- 3.Iscove NN, Sieber F, Winterhalter KH: Erythroid colony formation in cultures of mouse and human bone marrow: analysis of the requirement for erythropoietin by gel filtration and affinity chromatography on agarose-concanavalin A. J Cell Physiol 83: 309–320, 1974.PubMedCrossRefGoogle Scholar
- 4.McLeod DL, Shreeve MM, Axelrad AA: Induction of megakaryocyte colonies with platelet formation in vitro. Nature 261: 492–494, 1976.PubMedCrossRefGoogle Scholar
- 5.Metcalf D, MacDonald HR, Odartchenko N, et al: Growth of mouse megakaryocyte colonies in vitro. Proc Natl Acad Sci USA 72: 1744–1748, 1975.PubMedCrossRefGoogle Scholar
- 6.Nakeff A, Daniels-Mcqueen S: In vitro colony assay for a new class of megakaryocyte precursor: Colony-forming unit megakaryocyte (CFU-M). Proc Soc Exp Biol Med 151: 587–590, 1976.PubMedGoogle Scholar
- 7.Strome JE, McLeod DL, Shreeve MM: Evidence for the clonal nature of erythropoietic bursts: Application of an in situ method for demonstrating heterochromatin in plasma cultures. Exp Hematol: 1977. (In print)Google Scholar
- 8.Papayannopoulou TH, Brice M, Stamatoyan-nopoulos G: Stimulation of fetal hemoglobin synthesis in bone marrow cultures from adult individuals. Proc Natl Acad Sci USA 73: 2033–2037, 1976.Google Scholar
- 9.Worton RG, McCulloch EA, Till, JE: Physical separation of hemopoietic stem cells from cells forming colonies in culture. J Cell Physiol 74: 171–182, 1969.PubMedCrossRefGoogle Scholar
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© Springer-Verlag New York Inc. 1978