Ultrastructure Methods in Cuticle Research

  • M. Locke
  • P. Huie
Part of the Springer Series in Experimental Entomology book series (SSEXP)

Abstract

Insect cuticle was among the first biological objects studied by electron microscopy. Pore canals and lamellae were observed in hand-cut wedges of cockroach cuticle as early as 1942 (Richards and Anderson 1942). It was also found possible to view whole mounts of thin cuticle membranes such as those of tracheae and tracheoles (Richards and Korda 1950). The use of the electron microscope for cuticle problems has grown exponentially in the 1960s and 1970s from these early beginnings.

Keywords

Osmium Tetroxide Golgi Complex Electron Energy Loss Spectroscopy Cuticular Protein Pore Canal 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Agar AW (1974) The basic principles of the electron microscope. In: Glauert AM (ed) Practical methods in electron microscopy, Vol 2. American El-sevier, New York.Google Scholar
  2. Ainsworth SK, Karnovsky MJ (1972) An ultrastructural staining method for enhancing the size and electron opacity of ferritin in the sections. J His-tochem Cytochem 20:225–229.CrossRefGoogle Scholar
  3. Alderson RH (1974) Image recording and display. In: Glauert AM (ed) Practical methods in electron microscopy, vol 2. North-Holland/American Elsevier, Amsterdam/New York.Google Scholar
  4. Anderson TF (1969) Electron microscopy of micro-organisms. In: Polister AW (ed) Physical techniques in biological research. Vol III, Part A. Academic Press, New York.Google Scholar
  5. Baumeister W, Hahn M (1978) Specimen supports. In: Hayat MA (ed) Principles and techniques of electron microscopy: Vol 8, Biological applications. Van Nostrand-Reinhold, Princeton, N.J..Google Scholar
  6. Black JT (1974) The scanning electron microscope: Operating principles. In: Hayat MA (ed) Principles and techniques of electron microscopy: Vol. 1, Biological applications. Van Nostrand-Reinhold, Princeton, N.J..Google Scholar
  7. Branton D, Bullivant S, Gilula NB, Karnovsky MJ, Moor H, Mühlethaler K, Northcote DH, Packer L, Satir B, Satir P, Speth V, Staehlin LA, Steere RL, Winestein RS (1975) Freeze-etching nomenclature. Science 190:54–56.PubMedCrossRefGoogle Scholar
  8. Britton HTS, Robinson RA (1931) Universal buffer solutions and the dissociation constant of veronal. J Chem Sde 1456.Google Scholar
  9. Brown GL, Locke M (1978) Nucleoprotein localization by bismuth staining. Tissue cell 10:365–388.PubMedCrossRefGoogle Scholar
  10. Bullivant S (1973) Freeze-etching and freeze-fracturing. In: Koehler JK (ed) Advanced techniques in biological electron microscopy. Springer Verlag, New York, Heidelberg, Berlin, pp. 67–112..Google Scholar
  11. Caveney S, Podgorski C (1975) Intercellular communication in a positional field. Ultrastructural correlates and tracer analysis of communication between insect epidermal cells. Tissue Cell 7:559–574.PubMedCrossRefGoogle Scholar
  12. Chandler JA (1977) Wavelength dispersive X-ray microanalysis in biological research. In: Meek G A, Elder H Y (eds) Analytical and quantitative methods in microscopy. Cambridge University Press, London.Google Scholar
  13. Cohen AL (1974) Critical point drying. In: Meek G A, Elder HY (eds) Analytical and quantitative methods in microscopy. Cambridge University Press, London.Google Scholar
  14. Coleman JR, Terepka AR (1974) Preparatory methods for electron probe analysis. In: Hayat MA (ed) Principles and techniques of electron microscopy: Vol 4, Biological applications. Van Nostrand-Reinhold, Princeton, N.J..Google Scholar
  15. Colliex C, Trebbia P (1978) Electron energy loss spectroscopy in the electron microscope: Present state of affairs. In: Sturgess JM (ed) Electron microscopy 1978, vol III. Microscopical Society of Canada, Toronto, Canada.Google Scholar
  16. Cornelisse CJ, Van Duijn P (1973a) A new method for the investigation of the kinetics of the capture reaction in phosphatase cytochemistry. I. Theoretical aspects of the local formation of crystalline precipitates. J Histochem Cy-tochem 21:607–613.CrossRefGoogle Scholar
  17. Cornelisse CJ, Van Duijn P (1973b) A new method for the investigation of the kinetics of the capture reaction in phosphatase cytochemistry. II. Theoretical and experimental study of phosphate diffusion from thin polyacrylamide films. J Histochem Cytochem 21:614–622.PubMedCrossRefGoogle Scholar
  18. Czaninski Y, Catesson AM (1974) Polyphenoloxidases. In: Hayat MA (ed) Electron microscopy of enzymes: Vol 2, Principles and methods. Van Nostrand-Reinhold, Princeton, N.J..Google Scholar
  19. Danon D, Goldstein L, Marikovsky Y, Skutelsky E (1972) Use of cationized fer-ritin as a label of negative charges on cell surfaces. J Ultrastruct Res 38:500–510.PubMedCrossRefGoogle Scholar
  20. Dawson RMC, Elliott DC, Elliott WH, Jones, KM (eds) (1959) Data for biochemical research, pp 192-209. Oxford University Press, London and New York.Google Scholar
  21. de Iraldi AP, Suburo AM (1970) Electron staining of synaptic vesicles using the Champy-Maillet technique. J Micros 91:99–103.CrossRefGoogle Scholar
  22. Dunn RF (1978) Calibration of magnification in transmission electron microscopy. In: eriHayat MD (ed) Principles and techniques of electron micrscopy: vol 8, Biological applications. Van Nostrand-Reinhold, Princeton, N.J..Google Scholar
  23. Eppig JJ Jr (1974) Tyrosinase. In: Hayat MA (ed) Electron microscopy of enzymes: Vol 2, Principles and methods. Van Nostrand-Reinhold, Princeton NJ.Google Scholar
  24. Essner E (1973) Phosphatases. In: Hayat MA (ed) Electron microscopy of enzymes: Vol 1, Principles and methods. Van Nostrand-Reinhold, Princeton NJ.Google Scholar
  25. Essner E (1974) Hemoproteins. In: Hayat MA (ed) Electron microscopy of enzymes: Vol 1, Principles and methods. Van Nostrand-Reinhold, Princeton NJ.Google Scholar
  26. Farnell GC, Flint RB (1975) Photographic aspects of electron microscopy. In: Hayat MA (ed) Principles and techniques of electron microscopy: Vol 5, Biological applications. Van Nostrand-Reinhold, Princeton NJ.Google Scholar
  27. Farquhar M (1978) Recovery of surface membrane in anterior pituitary cells. J Cell Biol 77:R35–R42.PubMedCrossRefGoogle Scholar
  28. Friend DS (1969) Cytochemical staining of multivesicular body and Golgi vesicles. J Cell Biol 41:269–279.PubMedCrossRefGoogle Scholar
  29. Friend DS, Brassil GE (1970) Osmium staining of endoplasmic reticulum and mitochondria in the rat adrenal cortex. J Cell Biol 46:252–266.PubMedCrossRefGoogle Scholar
  30. Glauert AM (1975) Fixation, dehydration and embedding of biological specimens. In: Glauert AM (ed) Practical methods in electron microscopy. Vol. 3, Pt I. American Elsevier, New York.Google Scholar
  31. Gomori G (1955) Methods in enzymology, Vol 1. Academic Press, New York.Google Scholar
  32. Goodhew PJ (1973) Specimen preparation in materials science. In: Glauert AM (ed) Practical methods in electron microscopy, Vol 1, Pt I. American Elsevier, New York.Google Scholar
  33. Goodhew PJ (1975) Electron microscopy and analysis. Wykeham Publ London, Springer-Verlag, New York.Google Scholar
  34. Halcrow K (1978) Modified pore canals in the cuticle of Gammarus (Crustacea: Amphipoda); a study by scanning and transmission electron microscopy. Tissue Cell 10:659–670.PubMedCrossRefGoogle Scholar
  35. Haschemeyer RH, Meyers RJ (1972) Negative staining. In: Hayat MA (ed) Principles and techniques of electron microscopy: Vol 2, Biological applications. Van Nostrand-Reinhold, New York.Google Scholar
  36. Hayat MA (1970) Principles and techniques of electron microscopy: Vol 5, Biological applications. Van Nostrand-Reinhold, Princeton, New York NJ.Google Scholar
  37. Hayat MA (1974) Principles and techniques of scanning electron microscopy: Vol 2, Biological applications. Van Nostrand-Reinhold, Princeton, NJ.Google Scholar
  38. Hayat MA (1975) Positive staining for electron microscopy. Van Nostrand-Reinhold, Princeton NJ.Google Scholar
  39. Hayat MA (1978) Introduction to biological scanning electron microscopy. University Park Press, Baltimore.Google Scholar
  40. Hayes TL (1973) Scanning electron microscope techniques in biology. In: Koehler JK (ed) Advanced techniques in biological electron microscopy. Springer-Verlag, New York, Heidelberg, Berlin.Google Scholar
  41. Henkelman RM, Ottensmeyer FP (1974) An energy filter for biological electron microscopy. J Microsc 102:79–94.PubMedCrossRefGoogle Scholar
  42. Holmes W (1943) Silver staining of nerve axons in paraffin sections. Anat. Record 86:157.CrossRefGoogle Scholar
  43. Howden HF, Ling LEC (1974) Low-magnification study of uncoated specimens. In: Hayat AM (ed) Principles and techniques of electron microscopy: Vol 1, biological applications. Van Nostrand-Reinhold Co., Princeton NJ.Google Scholar
  44. Hutchinson TE (1977) Energy dispersive X-ray microanalysis. In: Meek GA, Elder HY (eds) Analytical and quantitative methods in microscopy. Cambridge University Press, London.Google Scholar
  45. Jouffrey B, Kihn Y, Perez JPh, Sevely J, Zanchi G (1978) On chemical analysis of thin films by energy loss spectroscopy. In: Sturgess JM (ed) Electron microscopy 1978, Vol III. Microscopical Society of Canada, Pubi., Toronto, Canada.Google Scholar
  46. Kessel RG, Shih CY (1974) Scanning electron microscopy in biology. Springer, New York.Google Scholar
  47. Lange RH (1976) Tilting experiments in the electron microscope. In: Hayat MA (ed) Principles and techniques of electron microscopy: Vol 6, Biological applications. Van Nostrand-Reinhold, Princeton NJ.Google Scholar
  48. Larsen WJ (1970) Genesis of mitochondria in the fat body of an insect. J Cell Biol 47:373–383.PubMedCrossRefGoogle Scholar
  49. Larsen WJ (1971) Cell remodeling in the fat body at metamorphosis. PhD dissertations, Case Western Reserve University.Google Scholar
  50. Locke M (1966) The structure and formation of the cuticulin layer in the epicu-ticle of an insect, Calpodes ethlius (Lepidoptera, Hesperiidae). J Morphol 118:461–494.PubMedCrossRefGoogle Scholar
  51. Locke M (1969a) The localization of a peroxidase associated with hard cuticle formation in an insect, Calpodes ethlius Stoll, Lepidoptera, Hesperiidae. Tissue cell 1(3):555–574.PubMedCrossRefGoogle Scholar
  52. Locke M (1969b) The structure of an epidermal cell during the formation of the protein epicuticle and the uptake of molting fluid in an insect. J Morphol 127:7–40.CrossRefGoogle Scholar
  53. Locke M, Collins JV (1965) The structure and formation of protein granules in the fat body of an insect. J Cell Biol 26:857–885.PubMedCrossRefGoogle Scholar
  54. Locke M, Collins JV (1968) Protein uptake into multivesicular bodies and storage granules in the fat body of an insect. J Cell Biol 36:453–483.PubMedCrossRefGoogle Scholar
  55. Locke M, Huie P (1972) The fiber components of insect connective tissue. Tissue Cell 4:601–612.PubMedCrossRefGoogle Scholar
  56. Locke M, Huie P (1975) Staining of the elastic fibers in insect connective tissue after tannic acid/glutaraldehyde fixation. Tissue Cell 7:211–216.PubMedCrossRefGoogle Scholar
  57. Locke M, Huie P (1976) The beads in the golgi complex/ endoplasmic reticulum region. J Cell Biol 70:384–394.PubMedCrossRefGoogle Scholar
  58. Locke M, Huie P (1977) Bismuth staining for light and electron microscopy. Tissue Cell 9:347–371.PubMedCrossRefGoogle Scholar
  59. Locke M, Huie P (1979) Apolysis and the turnover of plasma membrane plaques during cuticle formation in an insect. Tissue Cell 11:277–291.PubMedCrossRefGoogle Scholar
  60. Locke M, Huie P (1980) The nucleolus during epidermal development in an insect. Tissue Cell 12:175–194.PubMedCrossRefGoogle Scholar
  61. Locke M, Krishnan N (1971a) Hot alcoholic phosphotungstic acid and uranyl acetate as routine stains for thick and thin sections. J Cell Biol 50:550–556.PubMedCrossRefGoogle Scholar
  62. Locke M, Krishnan N (1971b) Distribution of phenoloxidases and polyphenols during cuticle formation. Tissue Cell 3:103–126.PubMedCrossRefGoogle Scholar
  63. Locke M, Krishnan N (1973) The formation of the ecdysial droplets and the ec-dysial membrane in an insect. Tissue Cell 5:441–450.PubMedCrossRefGoogle Scholar
  64. Locke M, McMahon JT (1971) The origin and fate of microbodies in the fat body of an insect. J Cell Biol 48:61–78.PubMedCrossRefGoogle Scholar
  65. Locke M, Sykes AK (1975) The role of the Golgi complex in the isolation and digestion of organelles. Tissue Cell 7:143–158.PubMedCrossRefGoogle Scholar
  66. Locke M, Krishnan N, McMahon JT (1971) A routine method for obtaining high contrast without staining sections. J Cell Biol 50:540–544.PubMedCrossRefGoogle Scholar
  67. Maser MD, Powell TE III, Philpott CW (1967) Relationships among pH, os-molality, and concentration of fixative solutions. Stain technol 42:175–182.PubMedGoogle Scholar
  68. Moor H, Miihlethaler K (1963) Fine structure in frozen-etched yeast cells. J Cell Biol 17:609–628.PubMedCrossRefGoogle Scholar
  69. Rambourg A, Hernandez W, Leblond CP (1969) Detection of complex carbohydrates in the Golgi apparatus of rat cells. J Cell Biol 40:395–414.PubMedCrossRefGoogle Scholar
  70. Reid N (1975) Ultramicrotomy. In: Glauert AM (ed) Practical methods in electron microscopy. North-Holland Publ, Amsterdam, Oxford/American El-sevier, New York.Google Scholar
  71. Revel JP, Karnovsky MJ (1967) Hexagonal array of subunits in intercellular junctions of the mouse heart and liver. J Cell Biol 33:No. 3 C7–12..PubMedCrossRefGoogle Scholar
  72. Reynolds ES (1963) The use of lead citrate of high pH as an electron-opaque stain in electron microscopy. J Cell Biol 17:208.PubMedCrossRefGoogle Scholar
  73. Richards AG, Anderson TF (1942) Electron microscope studies of insect cuticle with a discussion of the application of electron optics to this problem. J Morphol 71:135–183.CrossRefGoogle Scholar
  74. Richards AG, Korda FH (1950) Studies on arthropod cuticle. 4. An electron microscope survey of the intima of arthropod tracheae. Ann Entomol Soc Am 43:49–71.Google Scholar
  75. Rogers AW (1973) Techniques of autoradiography, 2nd edn. Elsevier, Amsterdam, London.Google Scholar
  76. Salpeter MM, Bachman L (1972) Autoradiography. In: Hayat MA (ed) Principles and techniques of electron microscopy. Vol 2, Biological applications. Van Nostrand-Reinhold, Princeton NJ.Google Scholar
  77. Salpeter MM, McHenry FA (1973) Electron microscopic autoradiography. In: Koehler JK (ed) Advanced techniques in biological electron microscopy. Springer Verlag, New York, Heidelberg, Berlin.Google Scholar
  78. Scharf D (1977) Magnifications. Schocken Books, New York.Google Scholar
  79. Shivers RR, Brightman MW (1976) Trans-glial channels in ventral nerve roots of crayfish. J Comp Neurol 167:1–26.PubMedCrossRefGoogle Scholar
  80. Simionescu N, Simionescu M (1976) Galloylglucoses of low molecular weight as mordants in electron microscopy. I and II. J Cell Biol 70:608–633.PubMedCrossRefGoogle Scholar
  81. Staehelin LA (1973) Analysis and critical evaluation of the information contained in freeze-etch micrographs. In: Benedetti EL, Favard P (eds) Freeze-etching techniques and applications. Société Française de Microscopie Électronique, Paris.Google Scholar
  82. Steere RL (1957) Electron microscopy of structural detail in frozen biological specimens. J Biophys Biochem Cytol 3:45–60.PubMedCrossRefGoogle Scholar
  83. Stolinski C, Breathnach AS (1975) Freeze-fracture replication of biological tissues. Techniques, interpretation and applications. Academic Press, New York.Google Scholar
  84. Sturgess JM, Katona E, Moscarello MA (1973) The Golgi complex I. Isolation and ultrastructure in normal rat liver. J Membrane Biol 12:367–384.CrossRefGoogle Scholar
  85. Walpole GS (1914) Hydrogen potentials of mixtures of acetic acid and sodium acetate. J Chem Soc 105:2501.Google Scholar
  86. Weavers BA (1975) The analytical electron microscope Emma-4. In: Hayat MA (ed) Principles and techniques of electron microscopy: Vol 5, Biological applications. Van Nostrand-Reinhold, Princeton NJ.Google Scholar
  87. Weibel ER, Bolender RP (1973) Stereological techniques for electron microscopic morphometry. In: Hayat MA (ed) Principles and techniques of electron microscopy. Vol 3, Biological applications. Van Nostrand-Reinhold Co., Princeton NJ.Google Scholar
  88. Whitson SW (1977) Zinc osmium iodide (ZnlOs): A modification of technique and progress toward a mechanism of staining. J Cell Biol 75:247a.Google Scholar
  89. Wischnitzer S (1973) The electron microscope. In: Hayat MA (ed) Principles and techniques of electron microscopy: Vol 3, Biological applications. Van Nostrand-Reinhold, Princeton, NJ.Google Scholar
  90. Wolosewick JJ, Porter KR (1979) Microtubular lattice of the cytoplasmic ground substance. J Cell Biol 82:114–139.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag New York Inc. 1980

Authors and Affiliations

  • M. Locke
  • P. Huie

There are no affiliations available

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