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Quantitation of Cells and Fibers in Histologic Sections of Arterial Walls: Advantages of Contour Tracing on a Digitizing Plate

  • Seymour Glagov
  • Joseph Grande
  • Draga Vesselinovitch
  • Christopher K. Zarins

Abstract

A major objective of the study of histologic sections is the elucidation of the relation of structure to function under normal and abnormal conditions. Utilizing appropriate staining procedures for identification of specific components, comparisons of sections taken in orthogonal planes and reconstructions from serial sections have provided insights into details of microarchitecture (Bernimoulin and Schroeder 1977; Elias and Henning 1967; Takahashi and Suwa 1978; Underwood 1970). Morphometric techniques may also be applied to individual histologic sections in order to derive quantitative estimates of tissue composition and to establish the shape, dimensions, and orientation of cell and tissue components. Extrapolations of volume and surface features of these components from measurements on cross-sectional profiles are based on a series of geometric considerations and measurement procedures that comprise the analytical method known as stereology.

Keywords

Histologic Section Section Thickness Point Counting Contour Method Rabbit Aorta 
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Copyright information

© Springer-Verlag New York, Inc. 1981

Authors and Affiliations

  • Seymour Glagov
  • Joseph Grande
  • Draga Vesselinovitch
  • Christopher K. Zarins

There are no affiliations available

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