Insect Neurochemistry and Neurophysiology · 1986 pp 179-182 | Cite as
Approaches to Generating Monoclonal Antibodies to the Prothoracicotropic Hormone in ManducaSexta
Abstract
The prothoracicotropic hormone (PTTH), the primary regulator of insect molting and metamorphosis, is a cerebral peptide which in at least two insects exists in multiple molecular forms. In the tobacco hornworm, Manduca sexta, two PTTHs (big ~28 kD and small ~7 kD) have been identified that activate larval and pupal prothoracic glands differentially (Bollenbacher et al., 1984). Similarly, in the silkworm, Bombyx mori, two comparable molecular weight classes of PTTH have been identified, with the small 4kD moiety apparently existing in three forms (Ishizaki et al., 1983; Nagasawa et al., 1984). The physiological significance of multiple forms is not known. In large part, the dearth of knowledge on PTTH has been a result of the difficulty encountered purifying the neurohormone.
Keywords
Weight Class Corpus Allatum Prothoracic Gland Queen Mary College Adjacent Brain TissuePreview
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References
- Agui N., Bollenbacher W.E., Granger N.A. and Gilbert L.I. (1980) Corpus allatum is release site for insect prothoracicotropic hormone. Nature 285, 669–670.CrossRefGoogle Scholar
- Bollenbacher W.E. and Granger N.A. (1985) Endocrinology of the prothoracicotropic hormone. In Comprehensive Insect Physiology, Biochemistry and Pharmacology, Vol. 7, Endocrinology I (Ed. by G.A. Kerkut and L.I. Gilbert ), pp. 109 - 152, Pergamon Press, Oxford.Google Scholar
- Bollenbacher W.E., Katahira E.J., O’Brien M.A., Gilbert L.I., Thomas M.K., Agui N. and Baumhover A.H. (1984) Insect prothoracicotropic hormones Evidence for two molecular forms. Science 224, 1243 - 1245.PubMedCrossRefGoogle Scholar
- Ishizaki H., Mizoguchi A., Fujishita M., Suzuki A., Moriya I., O’Oka H., Kataoka H., Isogai A., Nagasawa H., Tamura S. and Suzuki A. (1983) Species specificity of the insect prothoracicotropic hormone (PTTH): The presence of Bombyx- and Samia-specific PTTHs in the brain of Bombyx mori. Develop. Growth and Differ. 25, 593–600.Google Scholar
- Nagasawa H., Kataoka H., Isogai A., Tamura S., Suzuki A., Ishizaki H., Mizoguchi A., Fujiwara Y. and Suzuki A. (1984) Amino-terminal amino acid sequence of the silkworm prothoracicotropic hormone: Homology with insulin. Science 226, 1344–1345.Google Scholar