Isolation Techniques for Insect Neuropeptides
In this paper we review certain key isolation schemes for insect neurohormones, with an attempt to gain a perspective on the methods previously used, on the relative abundance of various neuropeptides, and how parameters such as in vitro metabolism of neuropeptides impact on planning their isolation. In addition, we briefly review key principles for isolation of neuropeptides first developed for vertebrate neuropeptides, and how experiences gained by researchers in these systems can improve efficiency of isolation of insect neuropeptides. Our review of the literature is selective rather than comprehensive.
KeywordsPurification Factor Hydrophobic Peptide Isolation Scheme Corpus Cardiaca Diuretic Hormone
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- Hayes T. K. and Keeley L. L. (1984) Isolation of insect neuropeptides using reversed-phase high performance liquid chromatography. In Insect Neurochemistry and Neurophysiology (Ed. by Borkovec A. B. and Kelly T. J.), pp. Plenum, New York.Google Scholar
- Kataoka H., Toschi A., Li J. P., Carney R. L., Schooley D. A. and Kramer S. J. (1989a) Identification of an allatotropin from adult Manduca sexta. Science 243, 1481–1483.Google Scholar
- Nagasawa H., Kataoka H., Hori Y., Isogai A., Tamura S., Suzuki A., Guo F., Zhong X., Mizoguchi A., Fujishita M., Takahashi S. Y., Ohnishi E. and Ishizaki H. (1984) Isolation and some characterization of the prothoracicotropic hormone from Bombyx mori. Gen. Comp. Endocrinol. 53, 143–152.Google Scholar
- Wilson K. J. (1988) Purification of proteins/peptides for structural studies. In Protein/Peptide Sequence Analysis: Current Methodologies (Ed. by Bhown A. S.), pp. CRC Press, Boca Raton, Florida.Google Scholar