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Isolation Techniques for Insect Neuropeptides

  • David A. Schooley
  • Hiroshi Kataoka
  • Steven J. Kramer
  • Anne Toschi
Part of the Experimental and Clinical Neuroscience book series (ECN)

Abstract

In this paper we review certain key isolation schemes for insect neurohormones, with an attempt to gain a perspective on the methods previously used, on the relative abundance of various neuropeptides, and how parameters such as in vitro metabolism of neuropeptides impact on planning their isolation. In addition, we briefly review key principles for isolation of neuropeptides first developed for vertebrate neuropeptides, and how experiences gained by researchers in these systems can improve efficiency of isolation of insect neuropeptides. Our review of the literature is selective rather than comprehensive.

Keywords

Purification Factor Hydrophobic Peptide Isolation Scheme Corpus Cardiaca Diuretic Hormone 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© The Humana Press Inc. 1990

Authors and Affiliations

  • David A. Schooley
    • 1
  • Hiroshi Kataoka
    • 2
  • Steven J. Kramer
    • 3
  • Anne Toschi
    • 3
  1. 1.Department of BiochemistryUniversity of NevadaRenoUSA
  2. 2.Dept. of Agricultural ChemistryUniversity of TokyoTokyoJapan
  3. 3.Sandoz Crop ProtectionPalo AltoUSA

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