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Subject: Serodiagnosis (use of known antigens as reagents to detect antibodies in patient sera for diagnosing recent or past viral infection).
Serodiagnostic Principles: Mechanism of antibody production, response patterns to pri-mary and recurrent infections, and significance of antibody to immunity in viral diseases.
Laboratory Methods: Neutralization, complement fixation, hemagglutination inhibition, immune adherence hemagglutination, passive agglutination, hemolysis in gel, radioimmunoassay, enzyme immunoassay, indirect immunofluorescence/fluoroimmunoassay, and Western immunoblotting.
Interpretation of Test Results: Significance of presence of antibody or change in antibody level. Value of immunoglobulin type-specific assays in viral serodiagnosis.
Problems and Pitfalls: Insensitive and nonspecific serodiagnostic tests resulting from heterologous cross-reactions, immunologic interference, substandard reagents, or improper test performance.
KeywordsHemagglutination Inhibition Complement Fixation Test Phate Buffer Saline Antigen Titration Antiviral Antibody
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