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Organization and Expression of Potyviral Genes

  • J. G. Shaw
  • A. G. Hunt
  • T. P. Pirone
  • R. E. Rhoads
Conference paper

Abstract

The potyviruses have long been favored subjects for study by plant virologists. They constitute the most numerous of the three dozen or so groups of plant viruses, and collectively are responsible for more damage to the world’s crop plants than is caused by the viruses of most of if not all the other groups. This was rather dramatically illustrated by a recently conducted international election of “favorite” filamentous plant viruses among several eminent virologists in which the potyviruses emerged with a clear “victory” (Milne, 1988). The ecological and epidemiological aspects of diseases caused by potyviruses, with their many fascinating but varied and complex considerations, have also prompted the major efforts in research that have been directed over many years to this group of viruses.

Keywords

Coat Protein Potato Virus Tobacco Etch Virus Zucchini Yellow Mosaic Virus Cylindrical Inclusion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Allison, R., Johnston, R.E., and Dougherty W.D. (1986). The nucleotide sequence of the coding region of tobacco etch virus genomic RNA: evidence for the synthesis of a single polyprotein. Virology 154, 9–20.PubMedCrossRefGoogle Scholar
  2. Allison, R.F., Dougherty, W.G., Parks, T.D., Willis L., Johnston, R.E., Kelly, M. and Armstrong, F.B. (1985). Biochemical analysis of the capsid protein gene and capsid protein of tobacco etch virus: N-terminal amino acids are located on the virion’s surface. Virology 147, 309–316.PubMedCrossRefGoogle Scholar
  3. Antignus, Y., Raccah B., Gal-On, A., and Cohen, S. (1989). Biological and serological characterization of zuc-chini yellow mosaic virus and watermelon mosaic virus 2 isolate in Israel. Phytoparasitica, in press.Google Scholar
  4. Argos, P. (1988). A sequence motif in many polymerases. Nucleic Acids Res. 16, 9909–9916.PubMedCrossRefGoogle Scholar
  5. Berger, P.H., and Pirone, T.P. (1986). The effect of helper component on the uptake and localization of potyviruses in Myzus persicae. Virology 153, 256–261.PubMedCrossRefGoogle Scholar
  6. Berger, P.H., Hunt A.G., Domier L.L., Hellmann, G.M., Stram Y., Thornbury, D.W. and Pirone, T.P. (1989). Expression in transgenic plants of a viral gene product that mediates insect transmission of potyviruses. Proc. Natl. Acad. Sci. USA in press.Google Scholar
  7. Carrington, J.C., and Dougherty W.G. (1987). Small nuclear inclusion protein encoded by a plant potyvirus genome is a protease. J. Virol. 61, 2540–2548.PubMedGoogle Scholar
  8. Carrington J.C., Cary S.M., Parks, T.D., and Dougherty, W.G. (1989a). A second proteinase encoded by a plant potyvirus genome. EMBO J. 8, 365–370.PubMedGoogle Scholar
  9. Carrington, J.C., Freed, D.D., and Sanders T.S. (1989b). Autoproteolytic processing of potyvirus proteinase HC-Pro in Escherichia coli and in vitro. J. Virol. in press.Google Scholar
  10. De Mejia, M.V.G., Hiebert, E., Purcifull D.E., Thornbury D.W., and Pirone, T.P. (1985). Identification of potyviral amorphous inclusion protein as a nonstructural, virus-specific protein related to helper component. Virology 142, 34–43.PubMedCrossRefGoogle Scholar
  11. Deom, C.M., Oliver, M.J., and Beachy, R.N. (1987). The 30-kilodalton gene product of tobacco mosaic virus potentiates virus movement. Science 237, 389–393.PubMedCrossRefGoogle Scholar
  12. Domier, L.L., Franklin, K.F., Shahabuddin M., Hellmann, G.M., Overmeyer, J.H., Hiremath, S.T., Siaw, M.F.E., Lomonossoff, G.P., Shaw, J.G. and Rhoads, R.E. (1986). The nucleotide sequence of tobacco vein mottling virus RNA. Nucleic Acids Res. 14, 5417–5430.PubMedCrossRefGoogle Scholar
  13. Domier, L.L., Franklin K.M., Hunt, A.G., Rhoads, R.E., and Shaw, J.G. (1989). Infectious in vitro transcripts from cloned cDNA of a potyvirus, tobacco vein mottling virus. Proc Natl. Acad Sci. USA 86, 3509–3513.PubMedCrossRefGoogle Scholar
  14. Dougherty, W. G., and Hiebert, E. (1980). Translation of potyviral RNA in a rabbit reticulocyte lysate: identification of nuclear inclusion proteins as products of tobacco etch virus RNA translation and cylindrical inclusion protein as a product of the potyvirus genome. Virology 104, 174–182.PubMedCrossRefGoogle Scholar
  15. Dougherty, W.G., Willis, L., and Johnston, R.E. (1985). Topographic analysis of tobacco etch virus capsid protein epitopes. Virology 144, 66–72.PubMedCrossRefGoogle Scholar
  16. Dougherty, W.G., and Carrington, J.C. (1988). Expression and function of potyviral gene products. Ann. Rev. Phytopath. 26, 123–143.CrossRefGoogle Scholar
  17. Dougherty, W.G., and Hiebert, E. (1980). Translation of potyvirus RNA in a rabbit reticulocyte lysate: cell-free translation strategy and a genetic map of the potyviral genome. Virology 104, 183–194.PubMedCrossRefGoogle Scholar
  18. Edwardson, J.R. (1974). Some properties of the potato virus Y group. Fla. Agric. Exp. Sta. Monogr. Ser. 4, 1–398.Google Scholar
  19. Goldbach, R. (1987). Genome similarities between plant and animal RNA viruses. Microbiol. Sci. 4, 197–202.PubMedGoogle Scholar
  20. Gorbalenya, A.E., Koonin E.V., Donchenko, A.P., and Blinov, V.M. (1988). A conserved NTP-motif in putative helicases. Nature 333, 22–22.PubMedCrossRefGoogle Scholar
  21. Gorbalenya, A.E., Koonin, E.V., Donchenko, A.P., and Blinov, V.M. (1989). Coronavirus genome: Prediction of putative functional domains in the non-structural polyprotein by comparative amino acid sequence analysis. Nucleic Acids Res. 17, 4847–4861.PubMedCrossRefGoogle Scholar
  22. Govier, D.A., and Kassanis, B. (1971). The role of helper virus in aphid transmission of potato aucuba mosaic virus and potato virus C. J. Gen. Virol. 13, 221–228.CrossRefGoogle Scholar
  23. Graybosch, R., Hellmann, G.M., Shaw, J.G., Rhoads, R.E., and Hunt, A.G. (1989). Expression of a potyvirus non-structural protein in transgenic tobacco. Biochem Biophys. Res. Commun. 160, 425–432.PubMedCrossRefGoogle Scholar
  24. Hammond, J., and Hammond, R.W. (1989). Molecular cloning, sequencing and expression in Escherichia coli of the bean yellow mosaic virus coat protein gene. J. Gen. Virol. 70, 1961–1974.PubMedCrossRefGoogle Scholar
  25. Hari, V., Siegel, A., Rozek, C., and Timberlake, W.E. (1979). The RNA of tobacco etch virus contains poly(A). Virology 92, 568–571.PubMedCrossRefGoogle Scholar
  26. Hari, V. (1981). The RNA of tobacco etch virus: further characterization and detection of protein linked to RNA. Virology 112, 391–399.PubMedCrossRefGoogle Scholar
  27. Harrison, B.D., and Robinson, D.J. (1988). Molecular variation in vector-borne plant viruses: epidemiological significance. Phil. Trans. Roy. Soc. London B321, 447–462.Google Scholar
  28. Hellmann, G.M., Shaw, J.G., Lesnaw, J.A., Chu, L.-Y., Pirone, T.P., and Rhoads, R.E. (1980). Cell-free translation of tobacco vein mottling virus RNA. Virology 106, 207–216.PubMedCrossRefGoogle Scholar
  29. Hellmann, G.M., Hiremath, S.T., Shaw, J.G., and Rhoads, R.E. (1986). Cistron mapping of tobacco vein mottling virus. Virology 151, 159–171. encoded protease. Virology 163, 554–562.PubMedCrossRefGoogle Scholar
  30. Hiebert, E., and Dougherty, W.G. (1988). Organization and expression of the viral genomes. In: “The Plant Viruses. Vol. 4. The Filamentous Plant Viruses” (R.G. Milne, Ed.) pp. 159–178. Plenum Press, New York, NY.Google Scholar
  31. Hill, J.H., and Benner, H.I. (1976). Properties of potyvirus RNAs: turnip mosaic, tobacco etch and maize dwarf mosaic virus. Virology 75, 419–432.PubMedCrossRefGoogle Scholar
  32. Hodgman, T.C. (1988). A new superfamily of replicative proteins. Nature 333, 22–23.PubMedCrossRefGoogle Scholar
  33. Kamer, G., and Argos, P. (1984). Primary structural comparison of RNA-dependent polymerases from plant, animal and bacterial viruses. Nucleic Acids Res. 12, 7269–7282.PubMedCrossRefGoogle Scholar
  34. Kitamura, N., Semler, B.L., Rothberg, P.G., Larsen, G.R., Adler, C.J., Dorner, A.J., Emini, E/A/. Hanecak, R., Lee, J.J., van der Werf, S., Anderson, C.W. and Wimmer, E. (1981). Primary structure, gene organization and polypeptide expression of poliovirus RNA. Nature 291, 541–553.Google Scholar
  35. Knuhtsen, H., Hiebert, E., and Purcifull, D.E. (1974). Partial purification and some properties of tobacco etch virus induced intranuclear inclusions. Virology 61, 200–209.PubMedCrossRefGoogle Scholar
  36. Langenberg, W.G. (1986). Virus protein association with cylindrical inclusions of two viruses that infect wheat. J. Gen. Virol. 67, 1161–1168.CrossRefGoogle Scholar
  37. Lain, S., Riechmann, J.L., and Garcia, J.A. (1989). The complete nucleotide sequence of plum pox potyvirus RNA. Virus Res. 13, 157–172.PubMedCrossRefGoogle Scholar
  38. Lecoq, H. (1986). A poorly aphid transmitted variant of zucchini yellow mosaic virus. Phytopathology 76, 1063.Google Scholar
  39. Leonard, D.A., and Zaitlin, M. (1982). A temperature-sensitive strain of tobacco mosaic virus defective in cell-to-cell movement generates an altered viral-encoded protein. Virology 117, 416–424.PubMedCrossRefGoogle Scholar
  40. Lesemann, D.-E. (1988). Cytopathology. In: “The Plant Viruses. Vol. 4. The Filamentous Plant Viruses” (R.G. Milne, Ed.) pp. 179–235. Plenum Press, New York, NY.Google Scholar
  41. Maiss, E., Timpe, U., Brisske, A., Jelkmann, W., Casper, R., Himmler, G., Mattanovich, D. and Katinger, H.W.D. (1989). The complete nucleotide sequence of plum pox virus RNA. J.Gen.Virol. 70, 513–524.PubMedCrossRefGoogle Scholar
  42. McDonald, J.G., and Bancroft, J.B. (1977). Assembly studies on potato virus Y and its coat protein. J. Gen. Virol. 35, 251–263.CrossRefGoogle Scholar
  43. Milne, R.G. (1988). The economic impact of filamentous plant viruses. In: “The Plant Viruses. Vol. 4. The Filamentous Plant Viruses” (R.G. Milne, Ed.) pp. 331–335. Plenum Press, New York, NY.Google Scholar
  44. Murant, A.F., Raccah, B., and Pirone, T.P. (1988). Transmission by vectors. In: “The Plant Viruses. Vol. 4. The Filamentous Plant Viruses” (R.G. Milne, Ed.) pp. 237–273. Plenum Press, New York, NY.Google Scholar
  45. Murphy, J.F., Rhoads, R.E., Hunt, A.G., and Shaw, J.G. (1989). Tobacco etch virus RNA has a 24-kDa VPg. Virology in press.Google Scholar
  46. Oh, C.-S., and Carrington, J.C. (1989). Identification of essential residues in potyvirus proteinase HC-Pro by site directed mutagenesis. Virology in press.Google Scholar
  47. Pirone, T.P., and Thornbury, D.W. (1983). Role of virion and helper component in regulating aphid transmission of tobacco etch virus. Phytopathology 73, 872–875.CrossRefGoogle Scholar
  48. Powell Abel, P., Nelson, R.S., Hoffmann, N., Rogers, S.G., Fraley, R.T., and Beachy, R.N. (1986). Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene. Science 232, 738–743.CrossRefGoogle Scholar
  49. Reihmann, J.L., Lain, S., and Garcia, J.A. (1989). The genome-linked protein and 5’ end RNA sequence of plum pox potyvirus. J. Gen. Virol. 70, 2785–2789.CrossRefGoogle Scholar
  50. Robaglia, C., Durand-Tardif, M., Tronchet, M., Boudazin, G., Astier-Manifacier, S., and Cassedelbart, F. (1989). Nucleotide sequence of potato virus Y (N strain) genomic RNA. J. Gen. Virol. 70, 935–947.PubMedCrossRefGoogle Scholar
  51. Sehnke, P.C., Mason, A.M., Hood, S.J., Lister, R.M., and Johnson, J.E. (1989). A “zinc-finger”-type binding domain in tobacco streak virus coat protein. Virology 168, 48–56.PubMedCrossRefGoogle Scholar
  52. Shahabuddin, M., Shaw, J.G., and Rhoads, R.E. (1988). Mapping of the tobacco vein mottling virus VPg cistron. Virology 163, 635–637.PubMedCrossRefGoogle Scholar
  53. Shukla, D.D., Strike, P.M., Tracy, S.L., Gough, K.H., and Ward, C.W. (1988). The N-terminus and C-terminus of the coat proteins of potyviruses are surface-located and the N-terminus contains the major virus-specific epitopes. J. Gen. Virol. 69, 1497–1508.CrossRefGoogle Scholar
  54. Shukla, D.D., and Ward, C.W. (1989). Identification and classification of potyviruses on the basis of coat protein sequence data and serology. Arch. Virol. in press.Google Scholar
  55. Siaw, M.F.E., Shahabuddin, M., Ballard, S., Shaw, J.G., and Rhoads, R.E. (1985). Identification of a protein covalently linked to the 5′ terminus of tobacco vein mottling virus RNA. Virology 142, 134–143.PubMedCrossRefGoogle Scholar
  56. Thornbury, D.W., Hellmann, G.M., Rhoads, R.E., and Pirone, T.P. (1985). Purification and characterization of potyvirus helper component. Virology 144, 260–267.PubMedCrossRefGoogle Scholar
  57. Tollin, P., and Wilson, H.R. (1988). Particle structure. In: “The Plant Viruses. Vol. 4. The Filamentous Plant Viruses” (R.G. Milne, Ed.) pp. 51–83. Plenum Press, New York, NY.Google Scholar
  58. Turpen, T. (1989). Molecular cloning of a potato virus Y genome: Nucleotide sequence homology in non-coding regions of potyviruses. J. Gen. Virol. 70, 1951–1960.PubMedCrossRefGoogle Scholar
  59. Wimmer, E. (1982). Genome-linked proteins of viruses. Cell 28, 199–201.PubMedCrossRefGoogle Scholar
  60. Zabel, P., Moerman, M., Lomonossoff, G., Shanks, M., and Beyreuther, K. (1984). Cowpea mosaic virus VPg: sequencing of radiochemically modified protein allows mapping of the gene on B RNA. EMBO J. 3, 1629–1634.PubMedGoogle Scholar
  61. Zimmern, D. (1988). Evolution of RNA viruses, vol. 2, Retrovirus, viroids and RNA recombination. In: “RNA Genetics” (Domingo, E., Holland, J.J., and Ahlquist, P., Eds.) pp. 211–240. CRC Press Inc, Boca Raton, FL.Google Scholar

Copyright information

© Springer-Verlag New York Inc. 1990

Authors and Affiliations

  • J. G. Shaw
    • 1
  • A. G. Hunt
    • 2
  • T. P. Pirone
    • 1
  • R. E. Rhoads
    • 3
  1. 1.Department of Plant PathologyUniversity of KentuckyLexingtonUSA
  2. 2.Department of Plant AgronomyUniversity of KentuckyLexingtonUSA
  3. 3.Department of Plant BiochemistryUniversity of KentuckyLexingtonUSA

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