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Environmental Control of the Synthesis and Activity of Aquatic Microbial Ectoenzymes

  • Ryszard J. Chróst
Part of the Brock/Springer Series in Contemporary Bioscience book series (BROCK/SPRINGER)

Abstract

The majority (>95%) of organic matter in aquatic environments is composed of polymeric, high-molecular-weight compounds (Allen, 1976; Romankevich, 1984; Cole et al., 1984; Thurman. 1985; Münster and Chróst, 1990). Because the passage of organic molecules across the microbial cytoplasmic membrane is an active process requiring specific transport enzymes (permeases), only small (low-molecular-weight) and simple molecules can be directly transferred from the environment into the cell (Rogers, 1961; Payne, 1980a; Geller, 1985). This means that only a small portion of the total dissolved organic matter (DOM) is readily utilizable in natural waters (Münster, 1985; Azam and Cho, 1987; Jørgensen, 1987), and that the majority of DOM cannot be directly transported to microbial cells because of the large size of its molecules.

Keywords

Lake Water Alkaline Phosphatase Activity Cytoplasmic Membrane Extracellular Enzyme Eutrophic Lake 
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References

  1. Aaronson, S. 1981. Chemical Communication at the Microbial Level, vol. 1. CRC Press Inc., Boca Raton. 184 pp.Google Scholar
  2. Allen, H.L. 1976. Dissolved organic matter in lakewater: characteristics of molecular weight size fractions and ecological implications. Oikos 27: 64–70.CrossRefGoogle Scholar
  3. Ammerman, J.W. and Azam, F. 1985. Bacterial 5’-nucleotidase in aquatic ecosystems: A novel mechanism of phosphorus regeneration. Science 227: 1338–1340.PubMedCrossRefGoogle Scholar
  4. Armstrong, F.B. 1983. Biochemistry, 2nd edition. Oxford University Press, New York. 653 pp.Google Scholar
  5. Azam, F. and B.C. Cho. 1987. Bacterial utilization of organic matter in the sea. pp. 261–281 in Fletcher, M., Gray, T.R.G. and Jones, J.G. (editors), Ecology of Microbial Communities, Cambridge University, Cambridge.Google Scholar
  6. Barman, T.E. 1969. Enzyme Handbook, vol. 2. Springer Verlag, Berlin. 928 pp.Google Scholar
  7. Bell, R.T. and J. Kuparinen. 1984. Assessing phytoplankton and bacterioplankton production during early spring in lake Erken, Sweden. Applied and Environmental Microbiology 48: 1221–1231.PubMedGoogle Scholar
  8. Bengtsson, G. 1988. The impact of dissolved amino acids on protein and cellulose degradation in stream waters. Hydrobiologia 164: 97–102.CrossRefGoogle Scholar
  9. Boethling, R.S. 1975. Regulation of extracellular protease secretion in Pseudomonas maltophilia. Journal of Bacteriology 123: 954–961.PubMedGoogle Scholar
  10. Botsford, J.L. 1981. Cyclic nucleotides in prokaryotes. Microbiological Reviews 45: 620–645.PubMedGoogle Scholar
  11. Bretaudiere, J.P. and T. Stillman. 1984. Alkaline phosphatases. pp. 75–82 in Bergmeyer, H.U. (editor), Methods of Enzymatic Analysis, vol. 4, Verlag Chemie, Weinheim.Google Scholar
  12. Burns, R.G. 1983. Extracellular enzyme-substrate interactions in soil. pp. 249–298 in Slater, J.H., Whittenbury, R., and Wimpenny, J.W.T. (editors), Microbes in Their Natural Environments. Cambridge University Press, London.Google Scholar
  13. Chróst, R.J. 1975. Inhibitors produced by algae as an ecological factor affecting bacteria in water ecosystems. I. Dependence between phytoplankton and bacteria development. Acta Microbiologica Polonica 7(B): 125–133.Google Scholar
  14. Chróst, R.J. 1981. The composition and bacterial utilization of DOC released by phytoplankton. Kieler Meeresforschung Sonderheft 5: 325–332.Google Scholar
  15. Chróst, R.J. 1986. Algal-bacterial metabolic coupling in the carbon and phosphorus cycle in lakes. pp. 360–366 in Meguar, F. and Gantar, M. (editors), Perspectives in Microbial Ecology, Slovene Society for Microbiology, Ljubljana.Google Scholar
  16. Chróst, R.J. 1988. Phosphorus and microplankton development in a eutrophic lake. Acta Microbiologica Polonica 37: 205–225.Google Scholar
  17. Chróst, R.J. 1989. Characterization and significance of ß-glucosidase activity in lake water. Limnology and Oceanography 34: 660–672.CrossRefGoogle Scholar
  18. Chróst, R.J. 1990a. Microbial ectoenzymes in aquatic environments. pp. 47–78 in Overbeck, J. and Christ, R.J. (editors), Aquatic Microbial Ecology: Biochemical and Molecular Approaches, Springer Verlag, New York. 190 pp.Google Scholar
  19. Chróst, R.J. 1990b. Can bacteria affect the phytoplankton succession in lacustrine environments? pp. 15–20 in Burhardt, L. (editor), Evolution of Freshwater Lakes, Adam Mickiewicz University Press, Poznan.Google Scholar
  20. Chróst, R.J. 1991. Ectoenzymes in aquatic environments: microbial strategy for substrate supply. Verhandlungen der Internationalen Vereinigung für Theoretische and Angewandte Limnologie 24: 936–942.Google Scholar
  21. Chróst, R.J. and M.A. Faust. 1983. Organic carbon release by phytoplankton: its com- position and utilization by bacterioplankton. Journal of Plankton Research 5: 477–493.CrossRefGoogle Scholar
  22. Chróst, R.J., Münster, U., Rai, H., Albrecht, D., Witzel, P.K. and J. Overbeck. 1989. Photosynthetic production and exoenzymatic degradation of organic matter in euphotic zone of an eutrophic lake. Journal of Plankton Research 11: 223–242.CrossRefGoogle Scholar
  23. Chróst, R.J. and J. Overbeck. 1987. Kinetics of alkaline phosphatase activity and phosphorus availability for phytoplankton and bacterioplankton in lake Plußsee (north German eutrophic lake). Microbial Ecology 13: 229–248.CrossRefGoogle Scholar
  24. Chróst, R.J. and J. Overbeck. 1990. Substrate-ectoenzyme interaction: significance of β-glucosidase activity for glucose metabolism by aquatic bacteria. Archiv für Hydrobiologie Beihefte Ergebnisse Limnologie 34: 93–98.Google Scholar
  25. Chróst, R.J., Siuda, W., Albrecht, D. and J. Overbeck. 1986. A method for determining enzymatically hydrolyzable phosphate (EHP) in natural waters. Limnology and Oceanography 31: 662–667.CrossRefGoogle Scholar
  26. Chróst, R.J., Siuda, W. and G.Z. Hałemejko. 1984. Longterm studies on alkaline phosphatase activity (APA) in a lake with fish-aquaculture in relation to lake eutrophication and phosphorus cycle. Archiv für Hydrobiologie Supplement 70: 1–32.Google Scholar
  27. Chróst, R.J., Wciso, R. and G.Z. Hałemejko. 1986. Enzymatic decomposition of organic matter by bacteria in an eutrophic lake. Archiv für Hydrobiologie 107: 145–165.Google Scholar
  28. Chróst, R.J., Wciso, R. and J. Overbeck. 1988. Evaluation of the [3H]thymidine method for estimating bacterial growth rates and production in lake water: Re-examination and methodological comments. Acta Microbiologica Polonica 37: 95–112.Google Scholar
  29. Cole, J.J., McDowell, W.H. and G.E. Likens. 1984. Sources and molecular weight of dissolved organic carbon in an oligotrophic lake. Oikos 42: 1–9.CrossRefGoogle Scholar
  30. Crofton, P.M. 1982. Biochemistry of alkaline phosphatase isoenzymes. CRC Critical Reviews in Clinical and Laboratory Sciences 16: 161–194.CrossRefGoogle Scholar
  31. Daatselaar, M.C.C. and W. Harder. 1974. Some aspects of the regulation of the production of extracellular proteolytic enzymes by a marine bacterium. Archiv für Hydrobiologie 101: 21–34.Google Scholar
  32. Daniels, L.B. and R.H. Glew. 1984. β-Glucosidases in tissue. pp. 217–226 in Bergmeyer, H.U. (editor), Methods of Enzymatic Analysis, vol. 4, Verlag Chemie, Weinheim.Google Scholar
  33. Dobrogosz, W.J. 1981. Enzymatic activity. pp. 365–392 in Gerhardt, P., Murray, R.G.E., Costilow, R.N., Nester, E.W., Wood, W.A., Krieg, N.R. and Phillips, G.B. (editors), Manual of Methods for General Bacteriology. American Society for Microbiology, Washington DC.Google Scholar
  34. Dowd, J.E. and D.S. Riggs. 1965. A comparison of estimates of Michaelis-Menten kinetic constants from various linear transformations. Journal of Biological Chemistry 240: 863–869.PubMedGoogle Scholar
  35. Fogg, G.E. 1966. The extracellular products of algae. Oceanography and Marine Biology Annual Reviews 4: 195–205.Google Scholar
  36. Fogg, G.E. 1983. The ecological significance of extracellular products of phytoplankton photosynthesis. Botanica Marina 26: 3–14.CrossRefGoogle Scholar
  37. Francko, D. 1984. Phytoplankton metabolism and cyclic nucleotides. II. Nucleotide-induced perturbations of alkaline phosphatase activity. Archiv für Hydrobiology 100: 409–421.Google Scholar
  38. Gage, M.A. and E. Gorham. 1985. Alkaline phosphatase activity and cellular phosphorus as an index of the phosphorus status of phytoplankton in Minnesota lakes. Freshwater Biology 15: 227–233.CrossRefGoogle Scholar
  39. Geller, A. 1985. Degradation and formation of refractory DOM by bacteria during simultaneous growth on labile substrates and persistent lake water constituents. Swiss Journal of Hydrology 47: 27–44.CrossRefGoogle Scholar
  40. Glenn, A.R. 1976. Production of extracellular proteins by bacteria. Annual Reviews in Microbiology 30: 41–62.CrossRefGoogle Scholar
  41. Hałemejko, G.Z. and R.J. Chróst. 1984. The role of phosphatases in phosphorus mineralization during decomposition of lake phytoplankton blooms. Archiv für Hydrobiologie 101: 489–502.Google Scholar
  42. Hałemejko, G.Z. and R.J. Chróst. 1986. Enzymatic hydrolysis of proteinaceous particulate and dissolved material in an eutrophic lake. Archiv für Hydrobiologie 107: 1–21.Google Scholar
  43. Hancock, I.C. and I.R. Poxton. 1988. Bacterial Cell Surface Techniques. John Wiley and Sons, Chichester, 329 pp.Google Scholar
  44. Healey, F.P. and L.L. Hendzel. 1980. Physiological indicators of nutrient deficiency in lake phytoplankton. Canadian Journal of Fisheries and Aquatic Sciences 37: 442–453.CrossRefGoogle Scholar
  45. Hellebust, J.A. 1974. Extracellular products. pp. 838–863 in W.D.P. Stewart (editor), Algal Physiology and Biochemistry. Blackwell, Oxford.Google Scholar
  46. Hollibaugh, J.T. and Azam, F. 1983. Microbial degradation of dissolved proteins in seawater. Limnology and Oceanography 28: 1104–1116.CrossRefGoogle Scholar
  47. Holm-Hansen, O. 1984. Composition and nutritional mode of nanoplankton. Archiv für Hydrobiologie Beihefte Ergebnisse Limnologie 19: 125–129.Google Scholar
  48. Hoppe, H.G. 1983. Significance of exoenzymatic activities in the ecology of brackish water: measurements by means of methylumbelliferyl substrates. Marine Ecology Progress Series 11: 299–308.CrossRefGoogle Scholar
  49. Hoppe, H.G. 1986. Degradation in sea water. pp. 453–474 in Rehm, H.J. and Reed, G. (editors), Biotechnology, vol. 8, VCH Verlagsgesellschaft, Weinheim.Google Scholar
  50. Hoppe, H.G., Kim, S.J. and K. Gocke. 1988, Microbial decomposition in aquatic environments: combined processes of extracellular enzyme activity and substrate uptake. Applied and Environmental Microbiology 54: 784–790.PubMedGoogle Scholar
  51. Jacobsen, T.R. and H. Rai. 1988. Determination of aminopeptidase activity in lakewater by a short term kinetic assay and its application in two lakes of differing eutrophication. Archiv für Hydrobiologie 113: 359–370.Google Scholar
  52. Jørgensen, N.O.G. 1987. Free amino acids in lakes: concentrations and assimilation rates in relation to phytoplankton and bacterial production. Limnology and Oceanography 32: 97–111.CrossRefGoogle Scholar
  53. Karl, D.M. and M.D. Bailiff. 1989. The measurement and distribution of dissolved nucleic acids in aquatic environments. Limnology and Oceanography 34: 543–558.CrossRefGoogle Scholar
  54. King, G.M. 1986. Characterization of β-glucosidase activity in intertidal marine sediments. Applied and Environmental Microbiology 51: 373–380.PubMedGoogle Scholar
  55. Law, B.A. 1980. Transport and utilization of proteins by bacteria. pp. 381–409 in Payne, J.W. (editor), Microorganisms and Nitrogen Sources, John Wiley and Sons, New York.Google Scholar
  56. Lazdunski, M. 1974. “Half of the sites” reactivity and the role of subunit interactions in enzyme catalysis. pp. 81–140 in Kaiser, E.T. and Kezdy, F.J. (editors), Progress in Bioorganic Chemistry, vol. 3, John Wiley & Sons, New York.Google Scholar
  57. Leatherbarrow, R.J. 1987. Enzfitter. A Non-linear Regression Data Analysis Program for the IBM PC. Elsevier-Biosoft, Cambridge. 91 pp.Google Scholar
  58. Linden, G., Chappelet-Tordo, D. and M. Lazdunski. 1977. Milk alkaline phosphatase, stimulation by Mg2+ and properties of the Mg2+ site. Biochimica et Biophysica Acta 483: 100–106.PubMedGoogle Scholar
  59. Litchfield, C.D. and J.M. Prescott. 1976. Regulation of proteolytic enzyme production by Aeromonas proteolytica. II. Extracellular aminopeptidase. Canadian Journal of Microbiology 16: 23–27.CrossRefGoogle Scholar
  60. Little, J.E., Sjogren, R.E. and G.R. Carson. 1979. Measurement of proteolysis in natural waters. Applied and Environmental Microbiology 37: 900–908.PubMedGoogle Scholar
  61. Lundin, A., Amer, P. and J. Hellmer. 1989. A new linear plot for standard curves in kinetic substrate assays extended above the Michaelis-Menten constant: application to a luminometric assay of glycerol. Analytical Biochemistry 177: 125–131.PubMedCrossRefGoogle Scholar
  62. Maeda, M. and N. Taga. 1973. Deoxyribonuclease activity in seawater and sediment. Marine Biology 20: 58–63.CrossRefGoogle Scholar
  63. McComb, R.B., Bowers, G.N., Jr. and S. Posen. 1979. Alkaline Phosphatase. Plenum Press, New York. 358 pp.Google Scholar
  64. Mayer, L.M. 1989. Extracellular proteolytic enzyme activity in sediments of an intertidal mudflat. Limnology and Oceanography 34: 973–981.CrossRefGoogle Scholar
  65. Meyer-Reil, L.A. 1987. Seasonal and spatial distribution of extracellular enzymatic activities and microbial incorporation of dissolved organic substrates in marine sediments. Applied and Environmental Microbiology 53: 1748–1755.PubMedGoogle Scholar
  66. Morton, R.K. 1954. The purification of alkaline phosphatases of animal tissues. Biochemical Journal 57: 595–603.PubMedGoogle Scholar
  67. Murgier, M., Pelissier, C., Lazdunski, A. and C. Lazdunski. 1976. Existence, location and regulation of the biosynthesis of amino-endopeptidase in Gram-negative bacteria. European Journal of Biochemistry 65: 517–520.PubMedCrossRefGoogle Scholar
  68. Münster, U. 1985. Investigations about structure, distribution and dynamics of different organic substrates in the DOM of lake Plußsee. Archiv für Hydrobiologie Supplement 70: 429–480.Google Scholar
  69. Münster, U. and R.J. Chróst. 1990. Origin, composition and microbial utilization of dissolved organic matter. pp. 8–46 in Overbeck, J. and Chróst, R.J. (editors), Aquatic Microbial Ecology: Biochemical and Molecular Approaches. Springer Verlag, New York. 190 pp.Google Scholar
  70. Nikaido, H. and Nakae, T. 1979. The outer membrane of Gram-negative bacteria. Advances of Microbial Physiology 20: 163–250.CrossRefGoogle Scholar
  71. Paul, J.H., Jeffrey, W.H. and M.F. DeFlaun. 1987. Dynamics of extracellular DNA in the marine environment. Applied and Environmental Microbiology 53: 170–179.PubMedGoogle Scholar
  72. Paul, J.H., Jeffrey, W.H. and J.P. Cannon. 1990. Production of dissolved DNA, RNA, and protein by microbial populations in a Florida reservoir. Applied and Environmental Microbiology 56: 2957–2962.PubMedGoogle Scholar
  73. Payne, J.W. 1980a. Microorganisms and Nitrogen Sources. John Wiley and Sons, New York. 764 pp.Google Scholar
  74. Payne, J.W. 1980b. Transport and utilization of peptides by bacteria. pp. 211–256 in Payne, J.W. (editor), Microorganisms and Nitrogen Sources. John Wiley and Sons, New York.Google Scholar
  75. Pettersson, K. 1980. Alkaline phosphatase activity and algal surplus phosphorus as phosphorus-deficiency indicators in Lake Erken. Archiv für Hydrobiologie 89: 54–87.Google Scholar
  76. Priest, F.G. 1984. Extracellular Enzymes. Van Nostrand Reinhold (UK) Co. Ltd., Wokingham. 79 pp.Google Scholar
  77. Rego, V.J., Billen, G., Fontigny, A. and M. Somville. 1985. Free and attached proteolytic activity in water environments. Marine Ecology Progress Series 21: 245–249.CrossRefGoogle Scholar
  78. Riemann, B. and M. Sendergaard. 1986. Carbon Dynamics in Eutrophic,Temperate Lakes. Elsevier, Amsterdam. 345 pp.Google Scholar
  79. Rogers, H.J. 1961. The dissimilation of high molecular weight organic substrates. pp. 261–318 in Gunsalus, I.C. and Starrier, R.Y. (editors), The Bacteria, vol. 2, Academic Press, New York.Google Scholar
  80. Rogers, H.J., Perkins, H.R. and Ward, J.B. 1980. Microbial Cell Wall and Membranes. Chapman and Hall, London. 258 pp.Google Scholar
  81. Romankevich, E.A. 1984. Geochemistry of Organic Matter in the Ocean. Springer Verlag, Tokyo. 478 pp.Google Scholar
  82. Rosso, A.L. and F. Azam. 1987. Proteolytic activity in coastal oceanic waters: depth distribution and relationship to bacterial populations. Marine Ecology Progress Series 41: 231–240.CrossRefGoogle Scholar
  83. Siuda, W. 1984. Phosphatases and their role in organic phosphorus transformation in natural waters. A review. Polskie Archiwum Hydrobiologii 31: 207–233.Google Scholar
  84. Siuda, W. and R.J. Chróst. 1987. The relationship between alkaline phosphatase (APA) activity and phosphate availability for phytoplankton and bacteria in eutrophic lakes. Acta Microbiologica Polonica 36: 247–257.Google Scholar
  85. Smith, E.L. and R.L. Hill. 1960. Leucine aminopeptidase. pp. 37–62 in Boyer, P.D., Lardy, H. and Myrbäck, K. (editors), The Enzymes, vol. 4, Academic Press, New York.Google Scholar
  86. Somville, M. 1984. Measurement and study of substrate specificity of exoglucosidase activity in eutrophic water. Applied and Environmental Microbiology 48: 1181–1185.PubMedGoogle Scholar
  87. Somville, M. and G. Billen. 1983. A method for determining exoproteolytic activity in natural waters. Limnology and Oceanography 28: 190–193.CrossRefGoogle Scholar
  88. Stewart, A.J. and R.G. Wetzel. 1982. Phytoplankton contribution to alkaline phosphatase activity. Archiv für Hydrobiologie 93: 265–271.Google Scholar
  89. Suttle, C.A., Chan, A.M. and M.T. Cottrell. 1990. Infection of phytoplankton by viruses and reduction of primary productivity. Nature 347: 467–469.CrossRefGoogle Scholar
  90. Tamminen, T. 1989. Dissolved organic phosphorus regeneration by bacterioplankton: 5’nucleotidase activity and subsequent phosphate uptake in a mesocosm enrichment experiment. Marine Ecology Progress Series 5: 89–100.CrossRefGoogle Scholar
  91. Thurman, E.M. 1985. Organic Geochemistry of Natural Waters. Nijhoff/Junk, Boston. 687 pp.CrossRefGoogle Scholar
  92. Vincent, W.V. 1981. Rapid physiological assays for nutrient demand by the plankton. II. Phosphorus. Journal of Plankton Research 3: 699–710.CrossRefGoogle Scholar
  93. Wetzel, R.G. and G.E. Likens. 1979. Limnological Analyses. Saunders, Philadelphia. 395 pp.Google Scholar
  94. Wouters, J.T.M. and P.J. Bieysman. 1977. Production of some exocellular enzymes by Bacillus licheniformis 749/C in chemostat cultures. Federation of European Microbiological Societies Letters 1: 109–112.Google Scholar
  95. Wynne, D. and M. Gophen. 1981. Phosphatase activity in freshwater zooplankton. Oikos 37: 369–376.CrossRefGoogle Scholar

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  • Ryszard J. Chróst

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