Effect of Bacillus circulans D1 Thermostable Xylanase on Biobleaching of Eucalyptus Kraft Pulp

  • Daniela A. Bocchini
  • Valquiria B. Damiano
  • Eleni Gomes
  • Roberto Da Silva
Chapter
Part of the Applied Biochemistry and Biotechnology book series (ABAB)

Abstract

The alkalophilic Bacillus circulans Dl was isolated from decayed wood. It produced high levels of extracellular cellulase-free xylanase. The enzyme was thermally stable up to 60°C, with an optimal hydrolysis temperature of 70°C. It was stable over a wide pH range (5.5–10.5), with an optimum pH at 5.5 and 80% of its activity at pH 9.0. This cellulase-free xylanase preparation was used to biobleach kraft pulp. Enzymatic treatment of kraft pulp decreased chlorine dioxide use by 23 and 37% to obtain the same kappa number (κ number) and brightness, respectively. Separation on Sephadex G-50 isolated three fractions with xylanase activity with distinct molecular weights.

Index Entries

Bacillus circulans biobleaching kraft pulp thermophilic xylanase 
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References

  1. 1.
    Erikson, O., Goring, D. A. I., and Lindgren, B. O. (1980), Wood Sci. Technol. 14, 267–269.CrossRefGoogle Scholar
  2. 2.
    Ferreira Filho, E. X. (1994), Braz. J. Med. Biol. Res. 27, 1093–1109.PubMedGoogle Scholar
  3. 3.
    Tavares, V.B., Gomes, E., Iembo, T., Bocchini, D. A., and Da Silva, R. (1997), in Fifth Brazilian Symposium on the Chemistry of Lignins and other Wood Components, pp. 367–371.Google Scholar
  4. 4.
    Chen C. C., Adolphson, R., Dean, J. F. D., Eriksson, K. E., Adams, M. W. W., and Westpheling, J. (1997), Enzyme Microb. Technol. 20,39–45.CrossRefGoogle Scholar
  5. 5.
    Tolan, J. J. and Canovas, R. V. (1992), Pulp Paper Can. 93, 39–42.Google Scholar
  6. 6.
    Viikari, L., Buchert, J., Tenkanen, M., and Kantelinen, A. (1995), Bioresour. Technol. 50, 65–72.Google Scholar
  7. 7.
    Beg, Q. K., Bhushan, B., Kapoor, M., and Hoondal, G. S. (2000), Enzyme Microb. Technol. 27, 459–466.PubMedCrossRefGoogle Scholar
  8. 8.
    Dhillon, A., Gupta, J. K., Jauhari, B. M., and Khanna, S. (2000), Bioresour. Technol. 73, 273–277.CrossRefGoogle Scholar
  9. 9.
    Antonopoulos, V. T., Hernadez, M., Arias, M. E., Mavrakos, E., and Ball, A.S. (2001), Appl. Microbiol. Biotechnol. 57, 92–97.PubMedCrossRefGoogle Scholar
  10. 10.
    Da Silva, R. Yim, D. K. Park, Y. K. (1994) J. Ferment. Bioeng. 77, 109–111.CrossRefGoogle Scholar
  11. 11.
    Kulkarni, N., Abhay, S., and Rao, M. (1999), FEMS Microbiol. Rev. 23, 411–456.PubMedCrossRefGoogle Scholar
  12. 12.
    Tolan, J. J. and Canovas, R. V. (1992), Pulp Paper Can. 93, 39–42.Google Scholar
  13. 13.
    Wong, K. K. L, Tan, L. U. L, and Sadler, J. N. (1988), Microbiol. Rev. 52, 305–317.PubMedGoogle Scholar
  14. 14.
    Viikari, L., Ranua, M., Kantelinen, A., Sundquist, J., and Linko, M. (eds.) (1986), Proceedings of the 3rd International Conference on Biotechnology Pulp and Paper Industry, Stockholm, Sweden, pp. 67–69.Google Scholar
  15. 5.
    Eligir, G., Sykes, M., and Jeffries, T. W. (1995), Enzyme Microb. Technol. 17, 954–959.CrossRefGoogle Scholar
  16. 16.
    Patel, R. N., Grabski, A. C., and Jeffries, T. W. (1993), Appl. Microbiol. Biotechnol. 39, 405–412.CrossRefGoogle Scholar
  17. 17.
    Tavares, V. B., Gomes, E., and Da Silva, R. J. (1997), Brazilian Soc. Microbiol. 28, 179–182.Google Scholar
  18. 18.
    Senior, D. J., Meyers, P. R., Miller, D., Sutclije, R., Tan, L., and Saddler, J. N. (1988), Biotechnol. Lett. 10, 907–912.CrossRefGoogle Scholar
  19. 19.
    Yang, V. W., Zhaung, Z., Elegir, G., and Jeffries, T. W. J. (1995), Indust. Microb. 15, 434–441.CrossRefGoogle Scholar
  20. 20.
    Paice, M. G., Bernier, R., Jr. and Jurasek, L. (1988), Biotechnol. Bioeng. 32, 235–239.PubMedCrossRefGoogle Scholar
  21. 21.
    Buchert, J., Ranua, M., Kantelinen, A., and Viikari, L. (1992), Appl. Microb. Biotechnol. 37, 825–829.CrossRefGoogle Scholar
  22. 22.
    Garg, A. P., Roberts, J. C, and McCarthy, A. J. (1998), Enzyme Microb. Technol. 22, 594–598.CrossRefGoogle Scholar
  23. 23.
    Harris, G. W., Pickersgill, R. W., Connerton, I., Debeire, P., Touzel, J. P., Breton, C., and Pérez, S. (1997), Proteins: Struct. Funct. Genet. 29, 77–86.CrossRefGoogle Scholar
  24. 24.
    López, C., Blanco, A., and Pastor, F. I. J. (1998), Biotechnol. Lett. 20(3), 243–246.CrossRefGoogle Scholar
  25. 25.
    Bataillon, M., Cardinali, A. P. N., and Duchiron, F. (1998), Biotechnol. Lett. 20(11), 1067–1071.CrossRefGoogle Scholar
  26. 26.
    Silva, C. H. C., Puls, J., Sousa, M. V., and Filho, E. X. F. (1999), J. Braz. Soc. Microbiol. 30(2), 114–119.Google Scholar
  27. 27.
    Khanna, P., Sundari, S. S., and Kumar, N. J. (1995), World J. Microbiol. Biotechnol. 11, 242, 243.Google Scholar
  28. 28.
    Nakamura, S., Wakahayashi, K., Nakai, R., Aono, R., and Horikoshi, K. (1993), Appl. Environ. Microbiol. 59, 2311–2316.PubMedGoogle Scholar
  29. 29.
    Kang, M. K., Maeng, P. J., and Rhee, Y. H. (1996), Appl. Environ. Microbiol. 62(9), 3480–3482.PubMedGoogle Scholar
  30. 30.
    Breccia, J. D., Sinñeriz, F., Baigorí, M. D., Castro, G. R., and Kaul, R. H. (1998), Enzyme Microb. Technol. 22, 42–49.CrossRefGoogle Scholar
  31. 31.
    Horikoshi, K. (1973), Agric. Biol. Chem. 35(11), 1783–1791.CrossRefGoogle Scholar
  32. 32.
    Trevelyn, W. E., Procter, D. P., and Harrison, J. G. (1950), Nature 166, 444, 445.Google Scholar
  33. 33.
    Miller, G. L. (1959), Anal. Chem. 31, 426–428.CrossRefGoogle Scholar
  34. 34.
    Dhillon, A., Gupta, J. K., Khanna, S. (2000), Process Biochem. 35, 849–856.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2003

Authors and Affiliations

  • Daniela A. Bocchini
    • 1
  • Valquiria B. Damiano
    • 1
  • Eleni Gomes
    • 1
  • Roberto Da Silva
    • 1
  1. 1.Departmento de Quimica de Geociencias, Laboratório de Bioquímica dos Processos e Microbiologia AplicadaIBILCE-UNESPSão José do Rio PretoBrazil

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