Engineering Luciferases for Assays and Imaging

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Abstract

Luciferases have served a number of purposes in biomedical applications, including within reporter gene and split reporter complementation assays. These proteins, however, have not evolved for the purpose of biomedical research, and it is not surprising that the utility and robustness of these assays can be improved by protein engineering of the luciferase. In this chapter, we provide an overview of luciferases, protein engineering, and how protein engineering is applied to luciferases.

Keywords

Light Output Renilla Luciferase Protein Engineering Random Mutagenesis Codon Optimization 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer-Verlag London 2014

Authors and Affiliations

  1. 1.Department of RadiologyStanford University School of MedicineStanfordUSA
  2. 2.Department of Pharmaceutical SciencesEast Tennessee State UniversityJohnson CityUSA

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