Spatio-Temporal Signaling in Mast Cells

  • Bridget S. Wilson
  • Janet M. Oliver
  • Diane S. Lidke
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 716)

Abstract

This chapter summarizes the evidence for localized signaling domains in mast cells and basophils, with a particular focus on the high affinity IgE receptor, FcεRI and its crosstalk with other membrane proteins. It is noteworthy that a literature spanning 30 years established the FcεRI as a model receptor for studying activation-induced changes in receptor diffusion and lipid raft association. Now a combination of high resolution microscopy methods, including immunoelectron microscopy and sophisticated fluorescence-based techniques, provide new insight into the nanoscale spatial and temporal aspects of receptor topography on the mast cell plasma membrane. Physical crosslinking of FcεRI with multivalent ligands leads to formation of IgE receptor clusters, termed “signaling patches,” that recruit downstream signaling molecules. However, classes of receptors that engage solely withmono valent ligands can also form distinctive signaling patches. The dynamic relationships between receptor diffusion, aggregation state, clustering, signal initiation and signal strength are discussed in the context of these recent findings.

Keywords

Mast Cell Lipid Raft Bivalent Ligand Signaling Patch Mast Cell Membrane 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Landes Bioscience and Springer Science+Business Media 2011

Authors and Affiliations

  • Bridget S. Wilson
    • 1
  • Janet M. Oliver
    • 1
  • Diane S. Lidke
    • 1
  1. 1.Department of PathologyUniversity of New Mexico AlbuquerqueNew MexicoUSA

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