F4/80: The Macrophage-Specific Adhesion-GPCR and its Role in Immunoregulation
- 1k Downloads
Asamacrophage-restrictedreagent, the generation and application of the F4/80 mAb has greatly benefited the phenotypic characterization of mouse tissue macrophages for three decades. Following the molecular identification of the F4/80 antigen as an EGF-TM7 member of the adhesion-GPCR family, great interest was ignited to understand its cell type-specific expression pattern as well as its functional role in macrophage biology. Recent studies have shown that the F4/80 gene is regulated by a novel set of transcription factors that recognized a unique promoter sequence. Gene targeting experiments have produced two F4/80 knock out animal models and showed that F4/80 isnotrequired for normal macrophage development. Nevertheless, the F4/80 receptor was found to be necessary for the induction of efferent CD8+ regulatory T cells responsible for peripheral immune tolerance. The identification of cellular ligands for F4/80 and delineation of its signaling pathway remain elusive but are critical to understand the in vivo role of this macrophage-specific adhesion-GPCR.
KeywordsTreg Cell Cell Surface Glycoprotein Leukoc Biol Cellular Ligand Gene Target Experiment
Unable to display preview. Download preview PDF.
- 11.Gordon S. The Legacy of cell fusion. Oxford; New York: Oxford University Press; 1994.Google Scholar
- 15.Hume DA, Robinson AP, MacPherson GG et al. The mononuclear phagocyte system of the mouse defined by immunohistochemical localization of antigen F4/80. Relationship between macrophages, Langerhans cells, reticular cells and dendritic cells in lymphoid and hematopoietic organs. J Exp Med 1983; 158(5):1522–1536.PubMedCrossRefGoogle Scholar