Measuring Oxygen in Living Tissue: Intravascular, Interstitial, and “Tissue” Oxygen Measurements

  • David F. Wilson
  • Olga S. Finikova
  • Artem Y. Lebedev
  • Sophia Apreleva
  • Anna Pastuszko
  • William M. F. Lee
  • Sergei A. Vinogradov
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 701)

Abstract

Oxygen dependent quenching of phosphorescence has been used to measure the oxygen pressure in both the vasculature of the microcirculation and the interstitial spaces of resting muscle tissue. Oxygen sensitive molecules were either dissolved in the blood (intravascular space) or micro-injected into the interstitial space and the distributions, histograms, of the oxygen pressure were measured. The mean oxygen pressures are higher in the blood than in the interstitial space but the oxygen pressures in the lowest 10% of the two spaces were not significantly different, indicating there is minimal (< 1 mm Hg) oxygen gradient between the two spaces in the capillary bed.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • David F. Wilson
    • 1
  • Olga S. Finikova
    • 1
  • Artem Y. Lebedev
    • 1
  • Sophia Apreleva
    • 1
  • Anna Pastuszko
    • 1
  • William M. F. Lee
    • 2
  • Sergei A. Vinogradov
    • 1
  1. 1.Department of Biochemistry and BiophysicsUniversity of PennsylvaniaPhiladelphiaUSA
  2. 2.Department of MedicineUniversity of PennsylvaniaPhiladelphiaUSA

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