Measuring Oxygen in Living Tissue: Intravascular, Interstitial, and “Tissue” Oxygen Measurements
Oxygen dependent quenching of phosphorescence has been used to measure the oxygen pressure in both the vasculature of the microcirculation and the interstitial spaces of resting muscle tissue. Oxygen sensitive molecules were either dissolved in the blood (intravascular space) or micro-injected into the interstitial space and the distributions, histograms, of the oxygen pressure were measured. The mean oxygen pressures are higher in the blood than in the interstitial space but the oxygen pressures in the lowest 10% of the two spaces were not significantly different, indicating there is minimal (< 1 mm Hg) oxygen gradient between the two spaces in the capillary bed.
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- 5.Huang Ch-Ch, Lejevadi NS, Tammela O, Pastuszko A, Delivoria-Papadopoulos M, and Wilson DF. (1994) Relationship of extracellular dopamine in striatum of newborn piglets to cortical oxygen pressure. Neurochem. Res. 19: 640-655.Google Scholar
- 9.Richmond KN, Shonat RD, Lynch RM, and Johnson PC. (1999) Critical PO2 of skeletalmuscle in vivo. Am. J. Physiol. Heart Circ. Physiol. 277: H1831–H1840.Google Scholar
- 13.RumseyWL, Pawlowski M, Lejavardi N, andWilson DF. (1994) Oxygen pressure distribution in the heart in vivo and evaluation of the ischemic “border zone.” Am. J. Physiol. 266(4 Pt 2): H1676-80.Google Scholar
- 15.Shonat RD and Johnson PC. (1997) Oxygen tension gradients and heterogeneity in venous microcirculation: a phosphorescence quenching study. Am. J. Physiol. Heart Circ. Physiol. 272: H2233–H2240.Google Scholar
- 21.Vinogradov SA and Wilson DF. (1994) Metallotetrabenzoporphyrins. New phosphorescent probes for oxygen measurements. J. Chem. Soc., Perkin Trans. II, 103-111.Google Scholar