Signaling Cascades: Consequences of Varying Substrate and Phosphatase Levels

Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 736)

Abstract

We study signaling cascades with an arbitrary number of layers of one-site phosphorylation cycles. Such cascades are abundant in nature and integrated parts of many pathways. Based on the Michaelis–Menten model of enzyme kinetics and the law of mass-action, we derive explicit analytic expressions for how the steady state concentrations and the total amounts of substrates, kinase, and phosphatates depend on each other. In particular, we use these to study how the responses (the activated substrates) vary as a function of the available amounts of substrates, kinase, and phosphatases. Our results provide insight into how the cascade response is affected by crosstalk and external regulation.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Elisenda Feliu
    • 1
  • Michael Knudsen
    • 1
    • 2
  • Carsten Wiuf
    • 1
    • 2
  1. 1.Bioinformatics Research CentreAarhus UniversityAarhusDenmark
  2. 2.Centre for Membrane Pumps in Cells and Disease (PUMPKIN)Aarhus UniversityAarhusDenmark

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