Structural Studies of NEMO and TRAF6: Implications in NF-κB Activation

  • Hao Wu
  • Yu-Chih Lo
  • Qian Yin
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 691)


NF-κB proteins are evolutionarily conserved master regulators of immune and inflammatory responses [2]. They play critical roles in a wide array of biological processes including innate and adaptive immunity, oncogenesis, and development. They are activated in response to ligation of many receptors including T-cell receptors, B-cell receptors, members of the tumor necrosis factor (TNF) receptor superfamily, and the Toll-like receptor/interleukin-1 receptor (TLR/IL-1R) superfamily.


Zinc Finger Isothermal Titration Calorimetry Hydrophobic Patch High Order Oligomerization Fluorescence Energy Transfer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    Ea CK, Deng L, Xia ZP, Pineda G, Chen ZJ (2006) Activation of IKK by TNFalpha requires site-specific ubiquitination of RIP1 and polyubiquitin binding by NEMO. Mol Cell 22:245–257CrossRefPubMedGoogle Scholar
  2. 2.
    Gilmore TD (2006) Introduction to NF-kappaB: players, pathways, perspectives. Oncogene 25:6680–6684CrossRefPubMedGoogle Scholar
  3. 3.
    Lo YC, Lin SC, Rospigliosi CC, Conze DB, Wu C, Ashwell JD, Eliezer D, Wu H (2009) Structural basis for recognition of diubiquitins by NEMO. Mol Cell 33:602–615CrossRefPubMedGoogle Scholar
  4. 4.
    Scheidereit C (2006) IkappaB kinase complexes: gateways to NF-kappaB activation and transcription. Oncogene 25:6685–6705CrossRefPubMedGoogle Scholar
  5. 5.
    Tokunaga F, Sakata SI, Saeki Y, Satomi Y, Kirisako T, Kamei K, Nakagawa T, Kato M, Murata S, Yamaoka S et al (2009) Involvement of linear polyubiquitylation of NEMO in NF-kappaB activation. Nat Cell Biol 11:123–132CrossRefPubMedGoogle Scholar
  6. 6.
    Wu CJ, Conze DB, Li T, Srinivasula SM, Ashwell JD (2006) Sensing of Lys 63-linked polyubiquitination by NEMO is a key event in NF-kappaB activation [corrected]. Nat Cell Biol 8:398–406CrossRefPubMedGoogle Scholar
  7. 7.
    Yin Q, Lin SC, Lamothe B, Lu M, Lo YC, Hura G, Zheng L, Rich R, CamposAD, Myszka DG et al (2009) E2 interaction and dimerization in the crystal structure of TRAF6. Nat Struct Mol Biol 16:658–666CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Department of BiochemistryWeill Cornell Medical CollegeNew YorkUSA

Personalised recommendations