Different Methods for Culturing Biofilms In Vitro

  • S. Brook Peterson
  • Yasuhiko Irie
  • Bradley R. Borlee
  • Keiji Murakami
  • Joe J. Harrison
  • Kelly M. Colvin
  • Matthew R. ParsekEmail author


The field of biofilm microbiology, while by no means new, has been experiencing significant “growing pains” as more and more researchers become involved. One of the underlying reasons is the lack of standardized methods for culturing biofilm communities. Many times, the culturing format will be unique to the study in question, resulting in difficulties when other labs attempt to confirm results produced by another lab. Another issue has been the limited utility of different culturing methods for the specific research questions being asked. For example, culturing formats designed to be accessible to microscopy are not always suited for other types of analyses, such as harvesting biofilm biomass for biochemical measurements.


Flow Cell Planktonic Cell Polycarbonate Filter Bubble Trap Rotate Disk Reactor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • S. Brook Peterson
    • 1
  • Yasuhiko Irie
    • 1
  • Bradley R. Borlee
    • 1
  • Keiji Murakami
    • 1
  • Joe J. Harrison
    • 1
  • Kelly M. Colvin
    • 1
  • Matthew R. Parsek
    • 2
    Email author
  1. 1.Department of MicrobiologyUniversity of WashingtonSeattleUSA
  2. 2.Department of Microbiology - Box 357242, School of MedicineUniversity of WashingtonSeattleUSA

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