NIRS Measurement of O2 Dynamics in Contracting Blood and Buffer Perfused Hindlimb Muscle

  • Kazumi Masuda
  • Hisashi Takakura
  • Yasuro Furuichi
  • Satoshi Iwase
  • Thomas Jue
Conference paper

DOI: 10.1007/978-1-4419-1241-1_46

Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 662)
Cite this paper as:
Masuda K., Takakura H., Furuichi Y., Iwase S., Jue T. (2010) NIRS Measurement of O2 Dynamics in Contracting Blood and Buffer Perfused Hindlimb Muscle. In: Takahashi E., Bruley D. (eds) Oxygen Transport to Tissue XXXI. Advances in Experimental Medicine and Biology, vol 662. Springer, Boston, MA

Abstract

In order to obtain evidence that Mb releases O2 during muscle contraction, we have set up a buffer-perfused hindlimb rat model and applied NIRS to detect the dynamics of tissue deoxygenation during contraction. The NIRS signal was monitored on hindlimb muscle during twitch contractions at 1 Hz, evoked via electrostimulator at different submaximal levels. The hindlimb perfusion was carried out by perfusion of Krebs Bicarbonate buffer. The NIRS still detected a strong signal even under Hb-free contractions. The deoxygenation signal (Δ[deoxy]) was progressively increased at onset of the contraction and reached the plateau under both blood- and buffer-perfused conditions. However, the amplitude of Δ[deoxy] during steady state continued to significantly increase as tension increased. The tension-matched comparison of the Δ[deoxy] level under buffer-perfused and blood perfused conditions indicate that Mb can contribute approximately 50% to the NIRS signal. These results clarify the Mb contribution to the NIRS signal and show a falling intracellular PO2 as workload increases.

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Kazumi Masuda
    • 1
  • Hisashi Takakura
    • 2
  • Yasuro Furuichi
    • 3
  • Satoshi Iwase
    • 4
  • Thomas Jue
    • 5
  1. 1.Faculty of Human SciencesKanazawa UniversityKanazawaJapan
  2. 2.The Graduate School of Natural Science and TechnologyKanazawa UniversityKanazawaJapan
  3. 3.The graduate School of EducationKanazawa UniversityKanazawaJapan
  4. 4.Department of PhysiologyAichi Medical UniversityNagakuteJapan
  5. 5.Department of Biochemistry and Molecular MedicineUniversity of California DavisDavisUSA

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