Map-Based Cloning and Breeding Application of a Broad-Spectrum Resistance Gene Pigm to Rice Blast
Rice blast is one of the most destructive diseases of rice. The identification and utilization of broad-spectrum resistance genes has been the most effective and economical approach to control the disease. A native Chinese variety, GM4, was identified as a broad-spectrum and durable resistant resource. Genetic and mapping analysis indicated that blast resistance to nine isolates of different races in GM4 is controlled by the same dominant locus designated as Pigm. The map-based cloning strategy was employed with a large mapping population consisting of 1556 recessive individuals. Pigm was finally mapped on chromosome 6. The allelism test showed that Pigm was allelic to Pi-2 and Pi-9, two known blast resistance genes. A BAC contig covering the Pigm region was constructed and completely sequenced. An NBS-LRR gene cluster encompassing 10 NBS-LRR-type candidate resistance genes was identified in the 120-kb sequenced region, which contains 6 members in the Pi2 gene cluster. Sequence comparison of the orthologous and paralogous genes in the Pigm locus in both resistant and susceptible backgrounds showed that the Pigm loci had undergone duplication during the evolution of the resistance gene cluster. Furthermore, our analysis showed that Pigm confers broader-spectrum resistance to blast isolates from different rice regions than Pi 9/Pi2/Piz t /Piz, indicating its good potential in rice breeding for blast resistance. With molecular markers-assisted selection for Pigm, we have succeeded in developing elite hybrid rice lines with broad-spectrum blast resistance.
KeywordsBroad-spectrum resistance Pigm Gene cluster Resistance breeding
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