Understanding the Co-evolution of the Rice Blast Resistance Gene PI-TA and Magnaporthe oryzae Avirulence Gene AVR-PITA

  • Yulin Jia
  • Xueyan Wang
  • Stefano Costanzo
  • Seonghee Lee

Abstract

The Pi-ta gene in rice effectively prevents infection by races of Magnaporthe oryzae that contain the corresponding AVR gene, AVR-Pita. Pi-ta is a putative cytoplasmic protein with a centrally located nucleotide binding sites (NBS) and a leucine rich domain (LRD) at the carboxyl terminus. The Pi-ta gene has been deployed effectively in preventing rice blast in the southern US since 1990. AVR-Pita encodes a predicted metalloprotease, and its processed form, AVR-Pita176$, was shown to directly bind with the Pi-ta protein in triggering effective defense responses. Variants of AVR-Pita were identified in many contemporary M. oryzae races and in isolates collected during the last 30 years in the US. Sequence analysis of these AVR-Pita variants revealed that the AVR-Pita protein might be under diversified selection. Most recently, sequence analysis of the Pi-ta variants in six Oryza species (O. sativa, O. glaberrima, O. officialis, O. rufipogon, O. barthii and O. nivara) revealed that functional nucleotide polymorphism at the position of 918 (FNP918) is present among all these Oryzae species, and Pi-ta may be under balanced selection. Our results suggest that Pi-ta co-evolves with AVR-Pita, and that rice engages trench warfare with M. oryzae during the host and pathogen co-evolution.

Keywords

Pi-ta AVR-Pita Co-evolution Balanced selection 

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Yulin Jia
    • 1
  • Xueyan Wang
  • Stefano Costanzo
  • Seonghee Lee
  1. 1.USDA-ARS Dale Bumpers National Rice Research CenterStuttgartUSA

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