Doubled haploid production has become an important tool in cereal breeding and has helped in accelerating the development of improved cultivars. Genetic variation for salt tolerance is rare in contemporary semi-dwarf (sd-1) rice germplasm (cultivars and breeding lines). Some rice landraces exhibit greater tolerance to salt, but are agronomically unacceptable because of their tall stature. One such landrace, Pokalli was subjected to anther culture in an attempt to induce gametoclonal variation for agronomically important traits such as plant height. Over 100 green plants were regenerated from 2,000 cultured anthers. Among these, two doubled haploid lines (DHs) exhibited semi-dwarf stature. Mutations (induced during the culture procedure) in the semi-dwarfing gene sd-1 were confirmed by PCR using locus specific primers. The semi-dwarf DH lines were multiplied and checked for response to salinity in hydroponics (10 dS m−1) and field tests at IRRI, Philippines. The work demonstrates the effectiveness of combining doubled haploid protocols with mutation detection.
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Afza, R. et al. (2009). Induction of Semi-Dwarf, Salt Tolerant Rice Mutants from a Tall Salt Tolerant Indica Landrace. In: Touraev, A., Forster, B.P., Jain, S.M. (eds) Advances in Haploid Production in Higher Plants. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-8854-4_26
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