Newly developed gynogenesis and androgenesis methods are described for tef. The diminutive floral structures of tef are problematic and have necessitated the development of spikelet culture and more effective treatments. Media composition has been optimised. In the case of anther culture, radiation treatments of seed of donor plants enhances the level of androgenesis. Various ploidy levels are found in the regenerants: haploids, doubled haploids, tetraploids and octoploids. The regenerated plants show variation for useful agronomic traits. Doubled haploidy in tef offers opportunities for improved genetic studies, accelerated breeding, the development of useful mutants and the development of new ploidy levels for agricultural exploitation.
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