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MALDI/MS Comparison of Fe-NTA Immobilized Metal Affinity Chromatography and Commercially-Available Metal Oxide Affinity Resins for Phosphopeptide Enrichment

  • Matthew B. Gates
  • Kenneth B. Tomer
  • Leesa J. Deterding
Part of the NATO Science for Peace and Security Series A: Chemistry and Biology book series (NAPSA)

Abstract

Immobilized metal ion affinity chromatography in combination with mass spectrometry has been used to determine the extent of phosphorylation and the specific sites of phosphorylation on proteins. There are several advantages to this combined approach. Immobilized metal ion affinity chromatography is the use of resins with metal constituents and metal oxides to enrich and isolate phosphopeptides. By selectively enriching phosphopeptides on media prior to MS analyses, suppression effects can be greatly reduced. In this report, we have investigated several resins from various sources to assess the phosphopeptide enrichment capabilities of each prior to mass spectrometric analyses. The phosphopeptide enrichment capabilities of six different resins, Glygen TiO2, ZrO2, and mixed ZrO2 and TiO2 NuTips, Titansphere TiO2 resin, PhosTrap magnetic titanium beads, and a Fe-NTA resin, are compared.

Keywords

Mass Spectrometry Analysis Tryptic Peptide Enrichment Medium MALDI Mass Spectrum Phosphate Moiety 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science + Business Media B.V 2008

Authors and Affiliations

  • Matthew B. Gates
    • 1
  • Kenneth B. Tomer
    • 1
  • Leesa J. Deterding
    • 1
  1. 1.Laboratory of Structural Biology, National Institute of Environmental Health SciencesNational Institutes of HealthResearch Triangle ParkUSA

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