Structure, Processing, and Polymerization of Rainbow Trout Egg Vitelline Envelope Proteins
Mammalian and non-mammalian eggs are surrounded by a zona pellucida (ZP) and vitelline envelope (VE), respectively. The rainbow trout egg VE consists of three proteins, VEα, VEβ, and VEγ, which are related to mouse egg ZP proteins ZP1, ZP2, and ZP3. Mass spectrometry (MS) has been used extensively to identify the intramolecular disulfide linkages and the cellular site of proteolytic processing of trout VE proteins. Additionally, Blue Native-PAGE (BN-PAGE) has been used to investigate polymerization of purified trout VE proteins under non-denaturing conditions. Results of these experiments reveal that, despite ~400 million years separating the appearance of trout and mice, and the change from external to internal fertilization and development, VE and ZP proteins have a great deal in common.
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- Darie, C. C., W. G. Janssen, et al. (2007). “Purified trout egg vitelline envelope proteins VEbeta and VEgamma polymerize into homomeric fibrils from dimers in vitro.” Biochim Biophys Acta doi:10.1016/j.bbapap.2007.10.011.Google Scholar
- Dumont, J. N. and A. R. Brummett (1985). Egg envelopes in vertebrates. New York, Plenum press.Google Scholar
- Jovine, L., H. Qi, et al. (2006). Features that affect secretion and assembly of zona pellucidaglycoproteins during mammalian oogenesis. Nottingham, U. Press.Google Scholar
- Sugiyama, H., K. Murata, et al. (1999). “Formation of mature egg envelope subunit proteins from their precursors (choriogenins) in the fish, Oryzias latipes: loss of partial C-terminal sequences of the choriogenins.” J Biochem (Tokyo) 125(3): 469-75.Google Scholar
- Swamy, M., G. M. Siegers, et al. (2006). “Blue native polyacrylamide gel electrophoresis (BN-PAGE) for the identification and analysis of multiprotein complexes.” Sci STKE 2006 (345): p. l4.Google Scholar
- Wallace, R. A. (1985). Vitellogenesis and oocyte growth in nonmammalian vertebrates. New York, Plenum Press.Google Scholar