Candida famata (Debaryomyces hansenii)

  • Andriy A. Sibirny
  • Andriy Y. Voronovsky

Debaryomyces hansenii (teleomorph of asporogenous strains known as Candida famata ) belongs to the group of so named ‘ flavinogenic yeasts ’ capable of riboflavin oversynthesis during starvation for iron. Some strains of C. famata belong to the most flavinogenic organisms known (accumulate 20 mg of riboflavin in 1 ml of the medium) and were used for industrial production of riboflavin in USA for long time. Many strains of D. hansenii are characterized by high salt tolerance and are used for ageing of cheeses whereas some others are able to convert xylose to xylitol, anti-caries sweetener. Transformation system has been developed for D. hansenii. It includes collection of host recipient strains, vectors with complementation and dominant markers and several transformation protocols based on protoplasting and electroporation. Besides, methods of multicopy gene insertion and insertional mutagenesis have been developed and several strong constitutive and regulatable promoters have been cloned. All structural genes of riboflavin synthesis and some regulatory genes involved in this process have been identified. Genome of D. hansenii has been sequenced in the frame of French National program ‘Genolevure’ and is opened for public access


Riboflavin D. hansenii C. famata flavinogenic transformation insertional mutagenesis 


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© Springer Science + Business Media B.V 2009

Authors and Affiliations

  • Andriy A. Sibirny
    • 1
  • Andriy Y. Voronovsky
  1. 1.Institute of Cell BiologyNational Academy of Sciences of UkraineLvivUkraine

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