The Comparative Study Of The Overall Effect Of Crude Oil On Fish In Early Stages Of Development

  • Nijole Kazlauskiene
  • Milda Zita Vosyliene
  • Egle Ratkelyte
Part of the NATO Science for Peace and Security Series book series (NAPSC)

The overall toxic impact of crude oil on fish was evaluated in acute and chronic tests by use of rainbow trout (Oncorhynchus mykiss) (embryos, larvae and juveniles). Survival, physiological (heart rate, gill ventilation frequency) and haematological parameters of test objects were studied. Crude oil depending on concentration and exposure duration caused a significant increase in larval mortality, whereas embryos were less and juveniles were least sensitive to the impact of oil. Sublethal crude oil concentrations induced significant alterations in cardio-respiratory parameters of embryos and larvae, as well as in respiratory and haematological parameters of juvenile fish.


crude oil toxicity fish embryos larvae juveniles 


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  1. Albers, P., 1995, Petroleum and individual polycyclic aromatic Hydrocarbons, in: Handbook of Ecotoxicology. Hoffman, D. J., Rattner B. A., Burton G. A. Jr., Cairns J. Jr. Eds. Boca Raton, Florida, pp. 330-755.Google Scholar
  2. Barron, M. G., Carls, M. G., Heintz, R., and Rice, S. D., 2004, Evaluation of Fish early LifeStage Toxicity Models of chronic embryonic Exposures to Complex polycyclic aromatic Hydrocarbon Mixtures, Toxicol. Sci. 78:60-67.CrossRefGoogle Scholar
  3. Barron, M. G., Carls, M. G., Short, J. W., and Rice, S. D., 2003, Photoenhanced Toxicity of aqueous Phase and chemically dispersed, weathered Alaska North Slope crude oil to Pacific Herring Eggs and Larvae, Environ. Toxicol. Chem. 22:650-660.CrossRefGoogle Scholar
  4. Carls, M. G., Holland, L., Larsen, M., Lum, J. L., Mortensen, D. G., Wang, S. Y., Wertheimer, A. C., 1996, Growth, Feeding, and Survival of Pink Salmon Fry exposed to Food contaminated with crude Oil, Am. Fish. Soc. Symp. 18:608-618.Google Scholar
  5. Carls, M. G., Rice, S. D., and Hose, J. E., 1999, Sensitivity of Fish Embryos to weathered crude Oil: I. Low Level Exposure during Incubation causes Malformations, genetic Damage, and Mortality in Larval Pacific Herring (Clupea pallasi), Environ. Toxicol. Chem. 18:481-493.CrossRefGoogle Scholar
  6. Girling, A. E., Markarian, R. K., Bennett, D., 1992, Aquatic Toxicity Testing of Oil Products Some Recommendations, Chemosphere 24:1469-1472.CrossRefGoogle Scholar
  7. Heintz, R. A., Short, J. W., and Rice, S. D., 1999, Sensitivity of Fish Embryos to weathered crude Oil: II. Increased Mortality of Pink Salmon (Oncorhynchus gorbuscha) Embryos incubating downstream from weathered Exxon Valdez crude Oil, Environ. Toxicol. Chem. 18:494-503.CrossRefGoogle Scholar
  8. Heintz, R. A., Rice, S. D., Wertheimer, A. C., Bradshaw, R. F., Thrower, F. P., Joyce, J. E., Short, J. W., 2000, Delayed Effects on Growth and marine Survival of Pink Salmon Oncorhychus gorbuscha after Exposure to crude Oil during embrionic Life, Mar. Ecol. Prog. 208:205-216.CrossRefGoogle Scholar
  9. Hoong, Gin K. Y., Huda, K. Md., Lim, W. K., and Tkalich, P., 2001, An Oil Spill-food Chain Interaction Model for Coastal Waters, Mar. Pollut. Bull. 42:590-597.CrossRefGoogle Scholar
  10. Kazakov, R. V., 1998, Atlantic Salmon. Nauka, Sankt-Peterburg, pp. 575 (In Russian).Google Scholar
  11. Kazlauskiene, N., 2004, Classsification and possible Consequences of the sublethal Effects of heavy Metal model Mixture in Fish at early Ontogenesis, in: Macro and Trace Elements: Mengen- and Spurenelemente. 1. 22 Workshop. M. Anke, ed., Schubert-Verlag, Leipzig, pp. 1172-1177.Google Scholar
  12. Kazlauskiene, N., Svecevicius, G., Vosyliene, M. Z., Marciulioniene, D., Montvydiene, D., 2004, Comparative Study on Sensitivity of higher Plants and Fish to heavy Fuel Oil, Environ. Toxicol. 19:449-451.CrossRefGoogle Scholar
  13. Lockhart, W., Duncan, D., Billeck, B., Danell, R., and Ryan, M., 1996, Chronic Toxicity of the “water-soluble Fraction” of Norman Wells crude Oil to Juvenile Fish, Spill. Sci. Tech. 3:259-262.CrossRefGoogle Scholar
  14. Marty, G. C., Short, J. W., Dambach, D. M., Willits, N. H., Heintz, R. A., Rice, S. D., Stegeman, J. J., and Hinton, D. E., 1997, Ascites, Premature Emergence, increased Gonadal Cell Apoptosis, and Cytochrome P4501A Induction in Pink Salmon Larvae continuously exposed to Oil-contaminated Gravel during Development, Can. J. Zool. 75:989-1007.CrossRefGoogle Scholar
  15. Michailova, L. V., Kutdusova, N. A., Abdullina, G. X., Kozlov, A. B., 2001, Effect of oilpolluted sediments on embryonic development of Siberian sturgeon Acipenser Baeri Brandt. Abstracts of VIII Meeting of Hydrobiological Society of RAS, I, Kaliningrad, pp. 151-152 (In Russian).Google Scholar
  16. Moles, A., Rice, S., Korn, S., 1979, Sesitivity Aliaskan freshwater and andromous Fishes to Prudhoe Bay crude Oil and Benzene, Trans. Am. Fish. Soc. 108:408-414.CrossRefGoogle Scholar
  17. Pollino, C. A., and Holdway, D. A., 2002, Toxicity Testing of crude Oil and related Compounds using early Life Stages of the Crimson-spotted Rainbowfish (Melanotaenia fluviatilis), Ecotoxicol. Environ. Saf. 52:180-189.CrossRefGoogle Scholar
  18. Rice, S. D., Babcock, M. M., Brodersen, C. C., Carls, M. G., Gharett, J. A., Korn, S., Moles, A., Short, J. Lethal and sublethal Effects of the water-soluble Fraction of Cook Inlet crude Oil on Pacific Herring ( Clupea harengus pallasi) Reproduction, US Dep. Commer. NOAA Tech. Memo. NMFS F/NWC-111. 1987.Google Scholar
  19. Secombes, C. J., Fletcher, T.C., O’Flynn J. A., Costello, M. J., Stagg, R., and Houlihan, D. F., 1991, Immunocompetence as a Measure of the biological Effects of Sewage Sludge pollution in fish, Comp. Biochem. Physiol. C 100:133-136.CrossRefGoogle Scholar
  20. Svobodova, Z., and Vykusova, B., 1991, Diagnostics, Prevention and Therapy of Fish Diseases and Intoxications. eds., Research Institute of Fish Culture and Hydrobiology, Vodnany, pp. 270.Google Scholar
  21. Vosyliene, M. Z, Kazlauskiene, N., Svecevicius, G., 2003, Complex Study into the Effect of heavy Metal model Mixture on biological Parameters of Rainbow Trout Oncorhynchus mykiss, Environ. sci. & Pollut. res. 10:103-107.CrossRefGoogle Scholar
  22. Vosyliene, M. Z., Kazlauskiene, N., and Joksas, K., 2005, Toxic Effects of crude Oil combined with Oil Cleaner Simple Green on Yolk-sac Larvae and adult Rainbow Trout, Environ. Sci. & Pollut. res. 12(3):136-139.CrossRefGoogle Scholar
  23. Woodward, D. F., Riley R.G., and Smith, C.E., 1983, Accumulation, sublethal Effects, and safe Concentrations of a refined Oil as evaluated with Cutthroat Trout, Arch. Environ. Contam. Toxicol. 12:455-460.CrossRefGoogle Scholar

Copyright information

© Springer 2008

Authors and Affiliations

  • Nijole Kazlauskiene
    • 1
  • Milda Zita Vosyliene
    • 1
  • Egle Ratkelyte
    • 1
  1. 1.Institute of EcologyVilnius UniversityLithuania

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