Time-Resolved Delayed Chlorophyll Fluorescence to Study the Influence of Bicarbonate on a Green Algae Mutant Photosystem II
Investigations On Photosystem II (Psii) Electron Transfer Processes by Observing Transient Absorption Changes Are Challenging. They Require High Psii Concentrations, Extended Averaging and are Negatively Affected By The Strong Light Scattering of Various Sample Types. Avoiding These Problems, The Analysis of the Time-Resolved Delayed Fluorescence (Df) measurements has become an important tool to study quantitatively light-induced electron transfer as well as associated Processes (E.G. Proton Movements) at the Donor Side Of Psii. Inter Alia This method can provide insights in the functionally important inner-protein proton movements, which are hardly detectable by conventional spectroscopic approaches. The delayed emission of chlorophyll fluorescence was measured on both wild type and mutant of green algae psii membrane particles in the time domain from 10 μs to 60 ms after each flash of a train of nanosecond laser pulses. The influence of the psii-associated carbonic anhydrase (ca) on the donor side reactions was studied for a Chlamydomonas Reinhardtii Wild type and Ca-Free Mutant.
KeywordsBicarbonate carbonic anhydrase Chlamydomonas delayed chlorophyll fluorescence photosystem II prompt chlorophyll fluorescence
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