Effect of Action Potential on Photosynthesis and Proton Transport in the Plant Cell

  • Natalia Krupenina
  • Alexander Bulychev


Effects Of Plasma Membrane Action Potential (Ap) On External Ph And Chlorophyll (Chl) Fluorescence In Chara Corallina Cells Were Examined In The Absence And Presence Of An Electron Transport Inhibitor And Electron Acceptors. In Intact Cells, Energy-Dependent Quenching Qe Developed At High Photon Flux Densities (Pfd) And Appeared Transiently After Electrically Stimulated Ap At Moderate Pfd. Diuron (Dcmu) Fully Inhibited Qe Established By Light And Ap. The Artificial Cyclic Electron Flow Restored Qe Imposed By Light But Not The Electrically Induced Qe. Methyl Viologen (Mv) At Concentrations 0.1–0.83 Mm Had No Effect On Fluorescence And Quantum Efficiency Of Photosystem Ii (Psii) Until The First Excitatory Stimulus Was Applied. However, Triggering Ap In The Presence Of Mv Induced Rapid Qe Formation At Low Pfd Indicating That A Divalent Cation Mv Became Instantly Accessible To Thylakoids And Partially Diverted Electron Flow From Psi To Mv Reduction.


Characeae action potential nonphotochemical quenching electron transport methyl viologen permeability 


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  1. Fromm J, Lautner S (2007) Electrical signals and their physiological significance in plants. Plant Cell Environ 30:249-257.PubMedCrossRefGoogle Scholar
  2. Johnson CH, Shingles R, Ettinger WF (2006) Regulation and role of calcium fluxes in the chloroplast. In: Wise RR, Hoober JK (eds) The Structure and Function of Plastids. Springer, Dordrecht, The Netherlands.Google Scholar
  3. Krupenina NA, Bulychev AA (2007) Action potential in a plant cell lowers the light requirement for non-photo-chemical energy-dependent quenching of chlorophyll fluorescence. Biochim Biophys Acta 1767:781-788.PubMedCrossRefGoogle Scholar
  4. Létant SE, Schaldach CM, Johnson MR, Sawvel A, Bourcier WL, Wilson WD (2006) Pore conductivity control at the hundred-nanometer scale. Small 2:1504-1510.PubMedCrossRefGoogle Scholar
  5. Neuhaus HE, Stitt M (1989) Perturbation of photosynthesis in spinach leaf discs by low concentrations of methyl viologen. Planta 179:51-60.CrossRefGoogle Scholar
  6. Salvucci ME, Portis ARJ, Heber U, Ogren WL (1987) Stimulation of thylakoid energization and ribulose-bisphosphate carboxylase/oxygenase in Arabidopsis leaves by methyl viologen. FEBS Lett 221:215-220.CrossRefGoogle Scholar
  7. Schansker G, Toth SZ, Strasser RJ (2005) Methylviologen and dibromothymoquinone treatments of pea leaves reveal the role of photosystem I in the Chl a fluorescence rise OJIP. Biochim Biophys Acta 1706:250-261.PubMedCrossRefGoogle Scholar

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© Springer Science + Business Media, B.V. 2008

Authors and Affiliations

  • Natalia Krupenina
    • 1
  • Alexander Bulychev
    • 1
  1. 1.Faculty of BiologyMoscow State UniversityMoscowRussia

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