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Structural Analysis of an FtsH2/FtsH3 Complex Isolated from Synechocystis sp. PCC 6803

  • Myles Barker
  • Marko Boehm
  • Peter J. Nixon
  • Jon Nield
Conference paper

Abstract

The FtsH2 protease of the cyanobacterium Synechocystis PCC 6803 plays an important role in many physiological processes including selective D1 degradation during Photosystem II repair. To clarify the structure of FtsH2, a Glutathione-S-transferase (GST)-tagged derivative was isolated by affinity chromatography from Synechocystis 6803. Our results indicate that FtsH2 forms a complex with FtsH3 and that this complex is hexameric.

Keywords

Synechocystis FtsH structure electron microscopy photosystem II repair 

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Copyright information

© Springer Science + Business Media, B.V. 2008

Authors and Affiliations

  • Myles Barker
    • 1
  • Marko Boehm
    • 1
  • Peter J. Nixon
    • 1
  • Jon Nield
    • 1
  1. 1.Division of Biology and Division of Molecular Biosciences, Wolfson Laboratories, Faculty of Natural SciencesImperial College LondonLondonUK

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