Genetic Fidelity Analyses of In Vitro Propagated Cork Oak (Quercus suber L.)
In vitro propagation methods, such as somatic embryogenesis (SE), are very interesting approaches when compared with traditional propagation, which presents serious drawbacks. SE is frequently regarded as the best system for propagation of superior genotypes (Kim, 2000) mostly because both root and shoot meristems are present simultaneously in somatic embryos. Cork oak (Quercus suber L.), as other woody species, is recalcitrant concerning somatic embryogenesis (SE). Most of the successful studies regarding SE within this species used juvenile materials (Gallego et al., 1997; Hernandez et al., 1999; Toribio et al., 1999; Hornero et al., 2001a; Pinto et al., 2001). Only recently, SE was successfully and reproducibly induced from adult cork oak trees (Pinto et al., 2002; Lopes et al., 2006), opening perspectives for breeding programmes of selected genotypes of this species.
KeywordsSomatic Embryo Somatic Embryogenesis Somaclonal Variation Propidium Iodide Fluorescence Internal Reference Standard
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