Selective Suppression of Polymerase Chain Reaction and Its Most Popular Applications
This chapter is devoted to methods based on suppression polymerase chain reaction (PCR) effect (cDNA library construction starting from a small amount of total RNA; suppression subtractive hybridization, SSH; ordered differential display, ODD; Marathon cDNA RACE; genome walking; variants of coincidence cloning, CC; normalization of cDNA libraries; multiplex PCR, mPCR; to in vitro cloning). Taken together, these approaches allow one to analyze complex DNA samples, from searching sequences of interest to determining complete structures of the respective genes.
KeywordsSelective PCR suppression PCR suppression suppression of the PCR PS pan handle suppression adapter inverted repeats annealing temperature primer concentration suppression sequence preparation of full-size cDNA small amount of biological material selective amplification differential display ordered differential display targeted differential display shortcoming disadvantage normalized cDNA libraries evolutionary conserved sequences search for promoter sites chromosome walking in vitro cloning multiplex PCR
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