Keynotes on Membrane Proteomics

  • Thierry Rabilloud
Part of the Subcellular Biochemistry book series (SCBI, volume 43)

Keywords

Membrane Protein Sodium Dodecyl Sulfate Lipid Bilayer Intrinsic Membrane Protein Ionic Detergent 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Bell, A.W., Ward, M.A., Blackstock, W.P., Freeman, H.N., Choudhary, J.S., Lewis, A.P., Chotai, D., Fazel, A., Gushue, J.N., Paiement, J., Palcy, S., Chevet, E., Lafreniere-Roula, M., Solari, R., Thomas, D.Y., Rowley, A. and Bergeron, J.J. (2001) Proteomics characterization of abundant Golgi membrane proteins. J. Biol. Chem. 276, 5152–5165.PubMedCrossRefGoogle Scholar
  2. Blonder, J., Conrads, T.P., Yu, L.R., Terumuma, A., Janini, G.M., Issaq, H.J., Vogel, J. and Veenstra, T.D. (2004) A detergent- and cyanogen bromide-free method for integral membrane proteomics: application to Halobacterium purple membranes and human epidermis. Proteomics 4, 31–45.PubMedCrossRefGoogle Scholar
  3. Camacho-Carvajal, M.M., Wollscheid, B., Aebersold, R., Steimle, V. and Schamel, W.W. (2004) Two-dimensional Blue native/SDS gel electrophoresis of multi-protein complexes from whole cellular lysates: a proteomics approach. Mol. Cell. Proteomics 3, 176–182.PubMedCrossRefGoogle Scholar
  4. Chevallet, M., Santoni, V., Poinas, A., Rouquié, D., Fuchs, A., Kieffer, S., Rossignol, M., Lunardi, J., Garin, J. and Rabilloud, T. (1998) New zwitterionic detergents improve the analysis of membrane proteins by two-dimensional electrophoresis. Electrophoresis 19, 1901–1909.PubMedCrossRefGoogle Scholar
  5. Cross, G.A. (1990) Glycolipid anchoring of plasma membrane proteins. Ann. Rev. Cell. Biol. 6, 1–39.PubMedCrossRefGoogle Scholar
  6. Devreese, B., Vanrobaeys, F., Smet, J., Van Beeumen, J. and Van Coster, R. (2002) Mass spectrometric identification of mitochondrial oxidative phosphorylation subunits separated by two-dimensional blue-native polyacrylamide gel electrophoresis. Electrophoresis 23, 2525–2533.PubMedCrossRefGoogle Scholar
  7. Fischer, F., Wolters, D., Rogner, M. and Poetsch, A. (2006) Toward the complete membrane proteome: high coverage of integral membrane proteins through transmembrane peptide detection. Mol. Cell. Proteomics 5, 444–453.PubMedCrossRefGoogle Scholar
  8. Gevaert, K., Goethals, M., Martens, L., Van Damme, J., Staes, A., Thomas, G.R. and Vandekerckhove, J. (2003) Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides. Nat. Biotechnol. 21, 566–569.PubMedCrossRefGoogle Scholar
  9. Gygi, S.P., Rist, B., Gerber, S.A., Turecek, F., Gelb, M.H. and Aebersold, R. (1999) Quantitative analysis of complex protein mixtures using isotope-coded affinity tags. Nat. Biotechnol. 17, 994–999.PubMedCrossRefGoogle Scholar
  10. Hartinger, J., Stenius, K., Hogemann, D. and Jahn, R. (1996) 16-BAC/SDS-PAGE: a two-dimensional gel electrophoresis system suitable for the separation of integral membrane proteins. Anal. Biochem. 124, 126–133.CrossRefGoogle Scholar
  11. Iwata, S., Lee, J.W., Okada, K., Lee, J.K., Iwata, M., Rasmussen, B., Link, T.A., Ramaswamy, S. and Jap, B.K. (1998) Complete structure of the 11-subunit bovine mitochondrial cytochrome bcl complex. Science 281, 64–71.PubMedCrossRefGoogle Scholar
  12. Luche, S., Santoni, V. and Rabilloud, T. (2003) Evaluation of nonionic and zwitterionic detergents as membrane protein solubilizers in two-dimensional electrophoresis. Proteomics 3, 249–253.PubMedCrossRefGoogle Scholar
  13. Macfarlane, D.E. (1989) Two dimensional benzyldimethyl-n-hexadecylammonium chloride-sodium dodecyl sulfate preparative polyacrylamide gel electrophoresis: a high capacity high resolution technique for the purification of proteins from complex mixtures. Anal. Biochem. 176, 457–463.PubMedCrossRefGoogle Scholar
  14. Molloy, M.P., Herbert, B.R., Williams, K.L. and Gooley, A.A. (1999) Extraction of Escherichia coli proteins with organic solvents prior to two-dimensional electrophoresis. Electrophoresis 20, 701–704.PubMedCrossRefGoogle Scholar
  15. Moritz, R.L., Clippingdale, A.B., Kapp, E.A., Eddes, J.S., Ji, H., Gilbert, S., Connolly, L.M. and Simpson, R.J. (2005) Application of 2-D free-flow electrophoresis/RP-HPLC for proteomic analysis of human plasma depleted of multi high-abundance proteins. Proteomics 5, 3402–3413.PubMedCrossRefGoogle Scholar
  16. Nijtmans, L.G., Henderson, N.S. and Holt, I.J. (2002) Blue Native electrophoresis to study mitochondrial and other protein complexes. Methods 26, 327–334.PubMedCrossRefGoogle Scholar
  17. Novakova, Z., Man, P., Novak, P., Hozak, P. and Hodny, Z. (2006) Separation of nuclear protein complexes by blue native polyacrylamide gel electrophoresis. Electrophoresis 27, 1277–1287.PubMedCrossRefGoogle Scholar
  18. Rabilloud, T., Adessi, C., Giraudel, A. and Lunardi, J. (1997) Improvement of the solubilization of proteins in two-dimensional electrophoresis with immobilized pH gradients. Electrophoresis 18, 307–316.PubMedCrossRefGoogle Scholar
  19. Santoni, V., Molloy, M.P. and Rabilloud, T. (2000) Membrane proteins and proteomics: un amour imposssible? Electrophoresis 21, 1054–1070.PubMedCrossRefGoogle Scholar
  20. Schagger, H. and von Jagow, G. (1991) Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active form. Anal. Biochem. 199, 223–231.PubMedCrossRefGoogle Scholar
  21. Szponarski, W., Sommerer, N., Boyer, J.C., Rossignol, M. and Gibrat, R. (2004) Large-scale characterization of integral proteins from Arabidopsis vacuolar membrane by two-dimensional liquid chromatography. Proteomics 4, 397–406.PubMedCrossRefGoogle Scholar
  22. Verma, D.P.S., Cheon, C.I. and Hong, Z. (1994) Small GTP-binding proteins and membrane biogenesis in plants. Plant Physiol. 106, 1–6.PubMedGoogle Scholar
  23. Washburn, M.P., Wolters, D. and Yates, J.R. 3rd. (2001) Large-scale analysis of the yeast proteome by multidimensional protein identification technology. Nat. Biotechnol. 19, 242–247.PubMedCrossRefGoogle Scholar
  24. Wilson, D., Hall, M.E., Stone, G.C. and Rubin, R.W. (1977) Some improvements in two-dimensional gel electrophoresis of proteins. Protein mapping of eukaryotic tissue extracts. Anal. Biochem. 83, 33–44.PubMedCrossRefGoogle Scholar
  25. Wu, C.C., MacCoss, M.J., Howell, K.E. and Yates, J.R. 3rd. (2003) A method for the comprehensive proteomic analysis of membrane proteins. Nat. Biotechnol. 21, 532–538.PubMedCrossRefGoogle Scholar

Copyright information

© Springer 2007

Authors and Affiliations

  • Thierry Rabilloud
    • 1
  1. 1.INSERM U548France

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