Bacterioplankton communities of Crater Lake, OR: dynamic changes with euphotic zone food web structure and stable deep water populations

  • Ena Urbach
  • Kevin L. Vergin
  • Gary L. Larson
  • Stephen J. Giovannoni

Abstract

The distribution of bacterial and archaeal species in Crater Lake plankton varies dramatically over depth and with time, as assessed by hybridization of group-specific oligonucleotides to RNA extracted from lakewater. Nonmetric, multidimensional scaling (MDS) analysis of relative bacterial phylotype densities revealed complex relationships among assemblages sampled from depth profiles in July, August and September of 1997 through 1999. CL500-11 green nonsulfur bacteria (Phylum Chloroflexi) and marine Group I crenarchaeota are consistently dominant groups in the oxygenated deep waters at 300 and 500 m. Other phylotypes found in the deep waters are similar to surface and mid-depth populations and vary with time. Euphotic zone assemblages are dominated either by β-proteobacteria or CL120-10 verrucomicrobia, and ACK4 actinomycetes. MDS analyses of euphotic zone populations in relation to environmental variables and phytoplankton and zooplankton population structures reveal apparent links between Daphnia pulicaria zooplankton population densities and microbial community structure. These patterns may reflect food web interactions that link kokanee salmon population densities to community structure of the bacterioplankton, via fish predation on Daphnia with cascading consequences to Daphnia bacterivory and predation on bacterivorous protists. These results demonstrate a stable bottom-water microbial community. They also extend previous observations of food web-driven changes in euphotic zone bacterioplankton community structure to an oligotrophic setting.

Keywords

Crater Lake Bacterioplankton community structure Multidimensional scaling Green nonsulfur bacteria Marine Group I crenarchaeota Daphnia predation 

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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Ena Urbach
    • 1
  • Kevin L. Vergin
    • 1
  • Gary L. Larson
    • 2
  • Stephen J. Giovannoni
    • 1
  1. 1.Department of MicrobiologyOregon State UniversityCorvallisUSA
  2. 2.USGS Forest and Rangeland Ecosystem Science CenterCorvallisUSA
  3. 3.eMetagen CorporationMadisonUSA

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