Genetic Transformation of Barley (Hordeum Vulgare L.) by Co-Culture of Immature Embryos with Agrobacterium
Barley is one of the major and most widely distributed crops worldwide. It has already been used intensively as a model species for cereals in the area of classical genetics and is still today of extraordinary importance as an experimental object for fundamental and applied research. In past years, a tremendous amount of genetic resources have been generated which include genomic DNA sequences, full-length cDNAs and expressed sequence tags. In barley, sequences from more than twenty thousand different genes are available (1, 2). For a comprehensive functional analysis of an expressed sequence, a known, reliable transformation technology is required. By standard approaches such as overexpression, knock out, translational reporter gene-fusions or promoter-reporter gene combination, biological functions and expression patterns can be assigned to a given gene. In 2002, the world production of barley was 132,215,617 metric tonnes (3). Main producers are Australia, Europe, Canada, Russian Federation and Ukraine. Barley constitutes a key input for the malting industry and breweries as well as for livestock production. Recently, it has been shown that barley can be successfully employed as a bioreactor to produce large quantities of valuable protein (4).
KeywordsFusarium Head Blight Immature Embryo Donor Plant Barley Plant Transgenic Barley
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