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Genetic Transformation of Barley (Hordeum Vulgare L.) by Co-Culture of Immature Embryos with Agrobacterium

  • G. Hensel
  • J. Kumlehn

Abstract

Barley is one of the major and most widely distributed crops worldwide. It has already been used intensively as a model species for cereals in the area of classical genetics and is still today of extraordinary importance as an experimental object for fundamental and applied research. In past years, a tremendous amount of genetic resources have been generated which include genomic DNA sequences, full-length cDNAs and expressed sequence tags. In barley, sequences from more than twenty thousand different genes are available (1, 2). For a comprehensive functional analysis of an expressed sequence, a known, reliable transformation technology is required. By standard approaches such as overexpression, knock out, translational reporter gene-fusions or promoter-reporter gene combination, biological functions and expression patterns can be assigned to a given gene. In 2002, the world production of barley was 132,215,617 metric tonnes (3). Main producers are Australia, Europe, Canada, Russian Federation and Ukraine. Barley constitutes a key input for the malting industry and breweries as well as for livestock production. Recently, it has been shown that barley can be successfully employed as a bioreactor to produce large quantities of valuable protein (4).

Keywords

Fusarium Head Blight Immature Embryo Donor Plant Barley Plant Transgenic Barley 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • G. Hensel
    • 1
  • J. Kumlehn
    • 1
  1. 1.Institute of Plant Genetics and Crop Plant Research (IPK)Plant Reproductive Biology GroupGaterslebenGermany

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