Skip to main content
SpringerLink
Log in

C1q, a Subcomponent of the First Complement Component: Methods for the Isolation from Various Animals

  • Chapter
Reviews on Immunoassay Technology

Abstract

The principal biological role of the complement system, found in all vertebrate blood, appears to be as an effector mechanism in the immune defence against infection by microorganisms. It is a complete mechanism in which activation products of the complement components cause lysis of cellular antigens, attract phagocytic cells to the site of activation, and facilitate uptake and destruction by the phagocytes.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 74.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Similar content being viewed by others

References

  • Agnello, V., Winchester, R. J. and Kunkel, H. G. (1970). Precipitin reactions of the C1q component of complement with aggregated γ-globulin and immune complexes in gel diffusion. Immunology, 19, 909–919.

    PubMed Central  CAS  PubMed  Google Scholar 

  • Alexander, R. and Steiner, L. A. (1980). The first component of complement from the bullfrog, Rana catesbeiana: functional properties of and isolation of subcomponent C1q. J. Immunol., 124, 1418–1425.

    CAS  PubMed  Google Scholar 

  • Andrews, B. S., Shadforth, M., Cunningham, P. and Davis, J. S. IV (1981). Demonstration of a C1q receptor on the surface of human endothelial cells. J. Immunol., 127, 1075–1080.

    CAS  PubMed  Google Scholar 

  • Antes, U., Heinz, H. and Loos, M. (1984). Enzyme-linked immunosorbent assay for C1q in human serum by use of monoclonal antibodies. J. Immunol. Methods, 74, 299–306.

    Article  CAS  PubMed  Google Scholar 

  • Arlaud, G. J., Sim, R. B., Duplaa, A. and Colomb, M. G. (1979). Differential elution of C1q, and from human bound to immune aggregates. Use in rapid purification ofsubcomponents. Mol. Immunol., 16, 445–450.

    Article  CAS  PubMed  Google Scholar 

  • Assimeh, S. N., Bing, D. H. and Painter, R. H. (1974). A simple method for the isolation of the subcomponents of the first component of complement by affinity chromatography. J. Immunol., 113, 225–234.

    CAS  PubMed  Google Scholar 

  • Bartholomew, R. M., Esser, A. F. and Müller-Eberhard, H. J. (1978). Lysis of oncornaviruses by human serum. Isolation of the viral complement (C1) receptor and identification as p15E. J. Exp. Med., 147, 844–853.

    Article  CAS  PubMed  Google Scholar 

  • Bohnsack, J. F., Tenner, A. J., Laurie, G. W., Kleinman, H. K., Martin, G. R. and Brown, E. J. (1985). The C1q subunit of the first component of complement binds to laminin: A mechanism for the deposition and retention of immune complexes in basement membrane. Proc. Natl. Acad. Sci. USA, 82, 3824–3828.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Burger, D., Sriranganathan, N., McDonald, T. and Gorham, J. R. (1983). Isolation of virus and antibody containing immune complexes from mink with Aleutian disease by affinity chromatography of equine complement C1q. Amer. J. Vet. Res., 44, 86–90.

    CAS  PubMed  Google Scholar 

  • Calcott, M. A. and Müller-Eberhard, H. J. (1972). C1q protein of human complement. Biochemistry, 11, 3443–3450.

    Article  CAS  PubMed  Google Scholar 

  • Campbell, R. D., Booth, N. A. and Forthergill, J. E. (1979). Purification and characterization of subcomponent C1q of the first component of bovine complement. Biochem. J., 177, 531–540.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Claus, D. R., Siegel, J., Petras, K., Osmand, A. P. and Gewurz, H. (1977). Interaction of C-reactive protein with the first component of human complement. J. Immunol., 119, 187–192.

    CAS  PubMed  Google Scholar 

  • Cooper, N. R., Jensen, F. C., Welsh, R. M. and Oldstone, M. B. A. (1976). Lysis of RNA tumor virus by human serum: direct antibody-independent triggering of the classical complement pathway. J. Exp. Med., 144, 970–984.

    Article  CAS  PubMed  Google Scholar 

  • Cooper, N. R. and Morrison, D. C. (1978). Binding and activation of the first component of the human complement by lipid A region of lipopolysaccharides. J. Immunol., 120, 1862–1868.

    CAS  PubMed  Google Scholar 

  • Cuatrecasas, P. and Anfinsen, C. B. (1971). Affinity chromatography. Methods Enzymol., 22, 345–385.

    Article  Google Scholar 

  • Davis, B. J. (1964). Disc electrophoresis—II Method and application to human serum proteins. Ann. N.Y. Acad. Sci., 121, 404–427.

    Article  CAS  PubMed  Google Scholar 

  • Engvall, E. and Perlmann, P. (1972). Enzyme-linked immunosorbent assay, ELISA. III Quantitation of specific antibodies by enzyme labelled antiimmunoglobulin in antigen-coated tubes. J. Immunol., 109, 129–135.

    CAS  PubMed  Google Scholar 

  • Ewald, R. W. and Schubart, A. F. (1966). Agglutinating activity of the complement component C’1q in the F-II latex fixation test. J. Immunol., 97, 100–105.

    CAS  PubMed  Google Scholar 

  • Fairbanks, G., Steck, T. L. and Wallach, D. F. H. (1971). Electrophoretic analysis of the major polypeptides of human erythrocyte membrane. Biochemistry, 10, 2606–2617.

    Article  CAS  PubMed  Google Scholar 

  • Fiedel, B. A., Rent, R., Myhrman, R. and Gewurz, H. (1976). Complement activation by interaction of polyanions and polycations. II. Precipitation and role of IgG, C1q and C1-INH during heparin-protamin-induced consumption of complement. Immunology, 30, 161–169.

    PubMed Central  CAS  PubMed  Google Scholar 

  • Heusser, C., Boesman, M., Nordin, J. H. and Isliker, H. (1973). Effect of chemical and enzymatic radioiodination on in vitro human C1q activities. J. Immunol., 110, 820–828.

    CAS  PubMed  Google Scholar 

  • Hitschold, T., Golan, M. D., Rabs, U. and Loos, M. (1983). Purification and physicochemical properties of C1q from guinea pig serum. Mol. Immunol., 20, 213–221.

    Article  CAS  PubMed  Google Scholar 

  • Höffken, K., McLaughlin, P. J., Price, M. R., Preston, V. E. and Baldwin, R. W. (1978). Rat C1q: Similarity to human C1q in functional and compositional properties. Mol. Immunol., 15, 409–412.

    Google Scholar 

  • Ingham, K. C., Brew, S. A. and Miekka, S. I. (1983). Interaction of plasma fibronectin with gelatin and complement C1q. Mol. Immunol., 20, 287–295.

    Article  CAS  PubMed  Google Scholar 

  • Ishizaka, T., Ishizaka, K., Borsos, T. and Rapp, H. (1966). C’1 fixation by human isoagglutinins: fixation of C’1 by γG and γM but not by γA antibody. J. Immunol., 97, 716–726.

    CAS  PubMed  Google Scholar 

  • Isliker, H., Bing, D. H. and Hynes, R. O. (1981). Interactions of fibronectin with C1q, a subcomponent of the first component of complement. The Immune System, 2, 231–238.

    CAS  Google Scholar 

  • Kolb, W. P., Kolb, L. M. and Podack, E. R. (1979). C1q: Isolation from human serum in high yield by affinity chromatography and development of a highly sensitive hemolytic assay. J. Immunol., 122, 2103–2111.

    CAS  PubMed  Google Scholar 

  • Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680–685.

    Article  CAS  PubMed  Google Scholar 

  • Lepow, I. H., Naff, G. B., Todd, E. W., Pensky, J. and Hinz, C. F. (1963). Chromatographic resolution of the first component of human complement into three activities. J. Exp. Med., 117, 983–1008.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Liberti, P. A., Bausch, D. M. and Baillie, R. D. (1981). The incorporation of high pH euglobulin precipitation in the isolation of C1q. J. Immunol. Methods, 40, 243–245.

    Article  CAS  PubMed  Google Scholar 

  • Linder, E. (1981). Binding of C1q and complement activation by vascular endothelium. J. Immunol., 126, 648–658.

    CAS  PubMed  Google Scholar 

  • Loos, M. (1982a). The classical complement pathway: mechanism of activation of the first component by antigen-antibody complexes. Prog. Allergy, 30, 135–192.

    CAS  PubMed  Google Scholar 

  • Loos, M. (1982b). Antibody independent activation of C1, the first component of complement. Ann. Immunol. (Inst. Pasteur), 133C, 165–179.

    Article  CAS  Google Scholar 

  • Loos, M., Wellek, B., Thesen, R. and Opferkuch, W. (1978). Antibody-independent interaction of the first component of complement with Gram-negative bacteria. Infect. Immunity, 22, 5–9.

    CAS  Google Scholar 

  • Mancini, G., Carbonara, A. O. and Heremans, J. E. (1965). Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry, 2, 235–254.

    Article  CAS  PubMed  Google Scholar 

  • McDonald, T. L. and Burger, D. (1979). Purification of the subunit C1q from the first component of equine complement. Immunology, 37, 517–527.

    PubMed Central  CAS  PubMed  Google Scholar 

  • McManus, L. M. and Nakane, P. K. (1980). Isolation and characterization of mouse C1q. J. Immunol. Methods, 36, 159–171.

    Article  CAS  PubMed  Google Scholar 

  • Menzel, E. J., Smolen, J. S., Liotta, L. A. and Reid, K. B. M. (1981). Composition between antifibronectin-antibody, C1q and collagens for the collagen-binding region of fibronectin. Immunobiology, 160, 72 (Abst.).

    Google Scholar 

  • Müller-Eberhard, H. J. and Kunkel, H. G. (1961). Isolation of a thermolabile serum protein which precipitates γ-globulin aggregates and participates in immune hemolysis. Proc. Soc. Exp. Biol. Med., 106, 291–295.

    Article  PubMed  Google Scholar 

  • Müller-Eberhard, H. J. and Schreiber, R. D. (1980). Molecular biology and chemistry of the alternative pathway of complement. Adv. Immunol., 29, 1–53.

    Article  PubMed  Google Scholar 

  • Paul, S. M. and Liberti, P. A. (1978). Isolation and characterization of highly stable rabbit C1q. J. Immunol. Methods, 21, 341–353.

    Article  CAS  PubMed  Google Scholar 

  • Paul, S. M., Baillie, R. D. and Liberti, P. A. (1978). Solvent effects on the structure of rabbit C1q, a subcomponent of the first component of complement. J. Biol. Chem., 253, 5658–5664.

    CAS  PubMed  Google Scholar 

  • Pohl, D. A., Gibbons, J. J. Jr., Tsai, C. C. and Roodman, S. T. (1980). Isolation and purification of human C1q from plasma. J. Immunol. Methods, 36, 13–27.

    Article  CAS  PubMed  Google Scholar 

  • Reid, K. B. M. (1981). Preparation of human C1q, a subcomponent of the first component of the classical pathway of complement. Methods Enzymol., 80, 16–25.

    Article  CAS  Google Scholar 

  • Reid, K. B. M. (1983). Proteins involved in the activation and control of the two pathways of human complement. Biochem. Soc. Trans., 11, 1–12.

    Article  CAS  PubMed  Google Scholar 

  • Reid, K. B. M., Lowe, D. M. and Porter, R. R. (1972). Isolation and characterization of C1q, a subcomponent of the first component of complement, from human and rabbit sera. Biochem. J., 130, 749–763.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Reid, K. B. M. and Porter, R. R. (1976). Subunit composition and structure of subcomponent C1q of the first component of human complement. Biochem. J., 155, 19–23.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Reid, K. B. M. and Porter, R. R. (1981). The proteolytic activation systems of complement. Ann. Rev. Biochem., 50, 433–464.

    Article  CAS  PubMed  Google Scholar 

  • Sasaki, T., Ueda, M. and Yonemasu, K. (1982). The subcomponent C1q of the first component of guinea pig complement: purification and characterization. J. Immunol. Methods, 48, 121–131.

    Article  CAS  PubMed  Google Scholar 

  • Sasaki, T. and Yonemasu, K. (1984). Comparative studies on biological activities of subcomponents C1q of the first component of human, bovine, mouse and guinea pig complement. Biochim. Biophys. Acta, 785, 118–122.

    Article  CAS  PubMed  Google Scholar 

  • Schepers, G. and Weiner, E. M. (1984)., Human C1q: rapid isolation and quantitative determination by immunodiffusion. J. Immunol. Methods, 67, 185–192.

    Article  CAS  PubMed  Google Scholar 

  • Schultz, D. R., Arnold, P. I., Wu, M. C., Lo, T. M., Volanakis, J. R. and Loos, M. (1979). Isolation and partial characterization of polysaccharide in ant venom (Pseudomyrmex sp.) that activates the classical complement pathway. Mol. Immunol., 16, 253–264.

    Article  CAS  PubMed  Google Scholar 

  • Shelton, E., Yonemasu, K. and Stroud, R. M. (1972). Ultrastructure of the human complement component, C1q. Proc. Natl. Acad. Sci. USA, 69, 65–68.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Slayter, H. S., Alexander, R. and Steiner, L. A. (1983). Electron microscopy of the complement protein C1q from the bullfrog, Rana catesbeiana. Eur. J. Immunol., 13, 102–106.

    Article  CAS  PubMed  Google Scholar 

  • Sledge, C. R. and Bing, D. H. (1973). Purification of the human complement protein C1q by affinity chromatography. J. Immunol., 111, 661–666.

    CAS  PubMed  Google Scholar 

  • Stemmer, F. and Loos, M. (1984). Purification and characterization of human, guinea pig and mouse C1q by fast protein liquid chromatography (FPLC). J. Immunol. Methods, 74, 9–16.

    Article  CAS  PubMed  Google Scholar 

  • Suba, E. A. and Csákó, G. (1976). C1q(C1) receptor on human platelets: inhibition of collagen induced platelet aggregation by C1q(C1) molecules. J. Immunol., 117, 304–309.

    CAS  PubMed  Google Scholar 

  • Taranta, A., Weiss, H. S. and Franklin, E. C. (1961). Precipitating factor for aggregated γ-globulin in normal human sera. Nature, 189, 239–240.

    Article  CAS  PubMed  Google Scholar 

  • Tenner, A. J. and Cooper, N. R. (1980). Analysis of receptor-mediated C1q binding to human peripheral blood mononuclear cells. J. Immunol., 125, 1658–1664.

    CAS  PubMed  Google Scholar 

  • Tenner, A. J. and Cooper, N. R. (1981). Identification of types of cells in human peripheral blood that bind C1q. J. Immunol., 126, 1174–1179.

    CAS  PubMed  Google Scholar 

  • Tenner, A. J., Lesavre, P. H. and Cooper, N. R. (1981). Purification and radiolabelling of human C1q. J. Immunol., 127, 648–653.

    CAS  PubMed  Google Scholar 

  • Theofilopoulos, A. N. and Dixon, F. J. (1979). The biology and detection of immune complexes. Adv. Immunol., 28, 89–220.

    Article  CAS  PubMed  Google Scholar 

  • Volanakis, J. E. and Stroud, R. M. (1972). Rabbit C1q: purification, functional and structural studies. J. Immunol. Methods, 2, 25–34.

    Article  CAS  PubMed  Google Scholar 

  • Volanakis, J. E. and Kaplan, M. H. (1974). Interaction of C-reactive protein complexes with the complement system. II. Consumption of guinea pig complement by CRP complexes: requirement for human C1q. J. Immunol., 133, 9–17.

    Google Scholar 

  • Wautier, J. L., Souchon, H., Reid, K. B. M., Peltier, A. P. and Caen, J. P. (1977). Studies on the mode of reaction of the first component of complement with platelets. Interaction between collagen-like portion of C1q and platelets. Immunochemistry, 14, 763–766.

    Article  CAS  PubMed  Google Scholar 

  • Weber, K. and Osborn, M. (1969). The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J. Biol. Chem., 244, 4406–4412.

    CAS  PubMed  Google Scholar 

  • Yachnin, S., Rosenblum, D. and Chatman, D. (1964). Biological properties of polynucleotides. V. Studies on the inhibition of the first component of complement by polyinosinic acid; the interaction with C1q. J. Immunol., 93, 542–548.

    Google Scholar 

  • Yonemasu, K. and Stroud, R. M. (1971). C1q: rapid purification method for preparation of monospecific antisera and for biochemical studies. J. Immunol., 106, 304–313.

    CAS  PubMed  Google Scholar 

  • Yonemasu, K., Stroud, R. M., Niedermeir, W. and Butler, W. T. (1971). Chemical studies on C1q: a modulator of immunoglobulin biology. Biochem. Biophys. Res. Commun., 43, 1388–1394.

    Article  CAS  PubMed  Google Scholar 

  • Yonemasu, K. and Stroud, R. M. (1972). Structural studies on human C1q: non-covalent and covalent subunits. Immunochemistry, 9, 545–554.

    Article  CAS  PubMed  Google Scholar 

  • Yonemasu, K., Sasaki, T. and Shinkai, H. (1980). Purification and characterization of subcomponent C1q of the first component of bovine complement. J. Biochem., 88, 1545–1554.

    CAS  PubMed  Google Scholar 

  • Yonemasu, K. and Sasaki, T. (1981). Purification and characterization of subcomponent C1q of the first component of mouse complement. Biochem. J., 193, 621–629.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Yonemasu, K. and Sasaki, T. (1986). Purification, identification and characterization of chicken C1q, a subcomponent of the first component of complement. J. Immunol. Methods, 88, 245–253.

    Article  CAS  PubMed  Google Scholar 

Download references

Authors
  1. Kunio Yonemasu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Takako Sasaki
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Abteilung für Innere Medizin I, Universität Ulm, 7900, Ulm, FR Germany

    S. B. Pal

Copyright information

© 1989 The Editor and Contributors

About this chapter

Cite this chapter

Yonemasu, K., Sasaki, T. (1989). C1q, a Subcomponent of the First Complement Component: Methods for the Isolation from Various Animals. In: Pal, S.B. (eds) Reviews on Immunoassay Technology. Palgrave Macmillan, London. https://doi.org/10.1007/978-1-349-11009-4_2

Download citation

Publish with us

Policies and ethics

Search

Navigation