Abstract
The penetration of light into a photobioreactor and its relation to hydrogen production were analyzed using the photosynthetic bacterium, Rhodobacter sphaeroides. Two photo-bioreactor types were used to examine hydrogen evolution at various light penetration depths: the A-type was comprised of four compartments of bacterial suspension and the B-type consisted of two cell-immobilized gels. A large portion of the incident light energy was absorbed in the upper part of the reactor. In the first compartment (0–5 mm) of the A-type reactor, 69% of the incident light energy was absorbed; 21% was absorbed in the second (5–10 mm). About 75% of the incident light energy was entrapped in the front gel of the B-type reactor. The cells in the deep parts of both reactors showed higher light energy conversion efficiencies to hydrogen, although the light spectrum reaching the cells was altered and the intensity was low. Alteration of the light spectrum and light intensity upon passage of light through the reactor greatly affected hydrogen production. Excess light energy in the shallow region reduced the total efficiency of the reactor. Light reaching the deep part of the reactor (600–780 nm) could be used effectively for hydrogen production.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Garcia, A., Vernon, L.P., Ke, B., and Mollenhauer, H., 1968, Some structural and photochemical properties of Rhodopseudomonas palustris subchromatophore particles obtained by treatment with Triton X-100, Biochemistry, 7:319–325.
Hirayama, O., Uya, K., Hiramatsu, Y., Yamada, H., and Moriwaki, K., 1986, Photoproduction of hydrogen by immobilized cells of a photosynthetic bacterium, Rhodospirillum rubrum G-9 BM, Agric. Biol. Chem., 50:891–897.
Jahn, A., Keuntje, B., Dorffler, M., Klipp, W., and Oelze, J., 1994, Optimizing photoheterotrophic H2 production by Rhodobacter capsulatus upon interposon mutagenesis in the hupL gene, Appl. Microbiol. Biotechnol., 40:687–690.
Mao, X.Y., Miyake, J., and Kawamura, S., 1986, Screening photosynthetic bacteria for hydrogen production from organic acid, J. Ferment. Technol., 64:245–249.
Miyake, J., Tomizuka, N., and Kamibayashi, K., 1982, Prolonged photo-hydrogen production by Rhodospirillum ruburum, J. Ferment. Technol., 60:199–203.
Miyake, J., Mao, X.Y., and Kawamura, S., 1984, Photoproduction of hydrogen from glucose by a co-culture of a photosynthetic bacterium and Clostridium butyricum, J. Ferment. Technol., 62:531–535.
Miyake, J., and Kawamura, S., 1987, Efficiency of light energy conversion to hydrogen by the photosynthetic bacterium Rhodobacter sphaeroides, Int. J. Hydrogen Energy, 39:147–149.
Miyake, J., Asada, Y., and Kawamura, S., 1989, Nitrogenase, in Biomass Handbook, Hall, C.W., and Kitani, O. (eds.), Gordon and Breech Scientific Publishers, New York, pp. 362–370.
Miyamoto, K., 1994, Hydrogen production by photosynthetic bacteria and microalge, in Recombinant Microbes for Industrial and Agricultural Applications, Murooka, Y., and Imanaka, T. (eds.), Marcel Dekker, New York, pp. 771–786.
Nogi, Y., Akiba, T., and Horikoshi, K., 1985, Wavelength dependence of photoproduction of hydrogen by Rhodopseudomonas rutila, Agric. Biol. Chem., 49:35–38.
Shneour, E.A., 1962, Carotenoid pigment conversion in Rhodopseudomonas sphaeroides, Biochem. Biophys. Acta, 62:534–540.
Tsygankov, A.T., Hirata, Y., Miyake, M., Asada, Y., and Miyake, J., 1994, Photobioreactor with photosynthetic bacteria immobilized on porous glass for hydrogen photoproduction, J. Ferment. Bioeng., 77:575–578.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Plenum Press, New York
About this chapter
Cite this chapter
Nakada, E., Nishikata, S., Asada, Y., Miyake, J. (1998). Light Penetration and Wavelength Effect on Photosynthetic Bacteria Culture for Hydrogen Production. In: Zaborsky, O.R., Benemann, J.R., Matsunaga, T., Miyake, J., San Pietro, A. (eds) BioHydrogen. Springer, Boston, MA. https://doi.org/10.1007/978-0-585-35132-2_42
Download citation
DOI: https://doi.org/10.1007/978-0-585-35132-2_42
Publisher Name: Springer, Boston, MA
Print ISBN: 978-0-306-46057-9
Online ISBN: 978-0-585-35132-2
eBook Packages: Springer Book Archive