Production of Monoclonal Antibodies in E. coli

  • Dorothea E. Reilly
  • Daniel G. Yansura
Chapter
Part of the Biotechnology: Pharmaceutical Aspects book series (PHARMASP, volume XI)

Abstract

The number of monoclonal antibodies approved for use as therapeutic agents by regulatory agencies has increased in the past several years. Monoclonal antibodies are predicted to become an increasingly larger part of biopharmaceutical products, and perhaps dominate the market share by the end of the decade (Walsh 2006). Mammalian expression systems, such as Chinese Hamster Ovary cells (CHO), are currently the preferred system for producing full-length monoclonal antibodies. Fungal systems could become more of a contender for the production of antibodies if titers can be increased (Andersen and Reilly 2004). However, with fungal production systems, there may be concerns about potential non-native mammalian N-linked or O-linked glycosylation that could result in immunogenic responses in humans. Technology developed in recent years (Hamilton et al. 2003) could help to alleviate this concern.

Abbreviations

ADCC

Antibody dependent cellular cytotoxicity

bp

Base pairs

C1q

Subcomponent of C1, a complement activator

CDC

Complement dependent cytotoxicity

CHO

Chinese hamster ovary

E. coli

Escherichia coli

ELISA

Enzyme-linked immunosorbent assay

FcRn

Neonatal Fc receptor

IPTG

Isopropyl β-D-thiogalactopyranoside

IV

Intravenous

kDa

Kilodaltons

PBS

Phosphate buffered saline

SEC

Size-exclusion chromatography

TIR

Translation initiation region

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Copyright information

© American Association of Pharmaceutical Scientists 2010

Authors and Affiliations

  • Dorothea E. Reilly
    • 1
  • Daniel G. Yansura
    • 2
  1. 1.Early Stage Cell Culture, Genentech, Inc.South San FranciscoUSA
  2. 2.Antibody Engineering, Genentech, Inc.South San FranciscoUSA

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